Laser Microdissection Publication List

March 20, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection
Ma L, Li W, Song Q
Methods Mol Biol. 2017;1551:161-169. doi: 10.1007/978-1-4939-6750-6_9.

Adenocarcinoma arising in multiple hyperplastic polyps in a patient with Helicobacter pylori infection and hypergastrinemia during long-term proton pump inhibitor therapy
Anjiki H, Mukaisho KI, Kadomoto Y, Doi H, Yoshikawa K, Nakayama T, Vo DT, Hattori T, Sugihara H
Clin J Gastroenterol. 2017 Feb 3. doi: 10.1007/s12328-017-0714-7.

TP53 mutation-mediated genomic instability induces the evolution of chemoresistance and recurrence in epithelial ovarian cancer
Zhang M, Zhuang G, Sun X, Shen Y, Wang W, Li Q, Di W
Diagn Pathol. 2017 Feb 2;12(1):16. doi: 10.1186/s13000-017-0605-8.

RNA Research

Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus
Yamauchi M, Moriyama M, Hayashida JN, Maehara T, Ishiguro N, Kubota K, Furukawa S, Ohta M, Sakamoto M, Tanaka A, Nakamura S
PLoS One. 2017 Mar 9;12(3):e0173017. doi: 10.1371/journal.pone.0173017.

Severe preeclampsia is associated with alterations in cytotrophoblasts of the smooth chorion
Garrido-Gomez T, Ona K, Kapidzic M, Gormley M, Simón C, Genbacev O, Fisher SJ
Development. 2017 Feb 23. pii: dev.146100. doi: 10.1242/dev.146100.

Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
Pavličev M, Wagner GP, Chavan AR, Owens K, Maziarz J, Dunn-Fletcher C, Kallapur SG, Muglia L, Jones H
Genome Res. 2017 Feb 7. doi: 10.1101/gr.207597.116.

Proteomics

Assessment of renal response with urinary exosomes in patients with AL amyloidosis: A proof of concept
Ramirez-Alvarado M, Barnidge DR, Murray DL, Dispenzieri A, Marin-Argany M, Dick CJ, Cooper SA, Nasr SH, Ward CJ, Dasari S, Jiménez-Zepeda VH, Leung N
Am J Hematol. 2017 Mar 10. doi: 10.1002/ajh.24717.

A standardized method for lectin microarray-based tissue glycome mapping
Zou X, Yoshida M, Nagai-Okatani C, Iwaki J, Matsuda A, Tan B, Hagiwara K, Sato T, Itakura Y, Noro E, Kaji H, Toyoda M, Zhang Y, Narimatsu H, Kuno A
Sci Rep. 2017 Mar 6;7:43560. doi: 10.1038/srep43560

Nrf2 Improves Leptin and Insulin Resistance Provoked by Hypothalamic Oxidative Stress
Yagishita Y, Uruno A, Fukutomi T, Saito R, Saigusa D, Pi J, Fukamizu A, Sugiyama F, Takahashi S, Yamamoto M
Cell Rep. 2017 Feb 21;18(8):2030-2044. doi: 10.1016/j.celrep.2017.01.064.

Fluorescence Applications

Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'
Nakashima Y, Egami Y, Kimura M, Wakimoto T, Abe I
PLoS One. 2016 Oct 12;11(10):e0164468. doi: 10.1371/journal.pone.0164468. eCollection 2016.

Tracking the route of phenanthrene uptake in mosses: An experimental trial
Spagnuolo V, Figlioli F, De Nicola F, Capozzi F, Giordano S
Sci Total Environ. 2016 Sep 29. pii: S0048-9697(16)32104-0. doi: 10.1016/j.scitotenv.2016.09.174.

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Cancer Research

Physical and Functional Interactions between ELL2 and RB in the Suppression of Prostate Cancer Cell Proliferation, Migration, and Invasion
Qiu X, Pascal LE, Song Q, Zang Y, Ai J, O’Malley KJ, Nelson JB, Zhou W
Neoplasia, Mar 2017, Volume 19, Issue 3, Pages 207–215

MicroRNA-155 Controls Exosome Synthesis and Promotes Gemcitabine Resistance in Pancreatic Ductal Adenocarcinoma
Mikamori M, Yamada D, Eguchi H, Hasegawa S, Kishimoto T, Tomimaru Y, Asaoka T, Noda T, Wada H, Kawamoto K, Gotoh K, Takeda Y, Tanemura M, Mori M, Doki Y
Sci Rep. 2017 Feb 15;7:42339. doi: 10.1038/srep42339.

Adenocarcinoma arising in multiple hyperplastic polyps in a patient with Helicobacter pylori infection and hypergastrinemia during long-term proton pump inhibitor therapy
Anjiki H, Mukaisho KI, Kadomoto Y, Doi H, Yoshikawa K, Nakayama T, Vo DT, Hattori T, Sugihara H
Clin J Gastroenterol. 2017 Feb 3. doi: 10.1007/s12328-017-0714-7.

Neurosciences

Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons
Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod RJ, Dieterich DC, Blum R, Sendtner M
J Cell Biol. 2017 Feb 28. pii: jcb.201604117. doi: 10.1083/jcb.201604117.

Nrf2 Improves Leptin and Insulin Resistance Provoked by Hypothalamic Oxidative Stress
Yagishita Y, Uruno A, Fukutomi T, Saito R, Saigusa D, Pi J, Fukamizu A, Sugiyama F, Takahashi S, Yamamoto M
Cell Rep. 2017 Feb 21;18(8):2030-2044. doi: 10.1016/j.celrep.2017.01.064.

Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells
Cifuentes M, Baeza V, Arrabal PM, Visser R, Grondona JM, Saldivia N, Martínez F, Nualart F, Salazar K
Mol Neurobiol. 2017 Feb 13. doi: 10.1007/s12035-017-0434-5.

Clinical Applications

EBM regeneration and changes in EBM component mRNA expression in stromal cells after corneal injury
Santhanam A, Marino GK, Torricelli AAM, Wilson SE
Molecular Vision 2017; 23:39-51

Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus
Yamauchi M, Moriyama M, Hayashida JN, Maehara T, Ishiguro N, Kubota K, Furukawa S, Ohta M, Sakamoto M, Tanaka A, Nakamura S
PLoS One. 2017 Mar 9;12(3):e0173017. doi: 10.1371/journal.pone.0173017.

A standardized method for lectin microarray-based tissue glycome mapping
Zou X, Yoshida M, Nagai-Okatani C, Iwaki J, Matsuda A, Tan B, Hagiwara K, Sato T, Itakura Y, Noro E, Kaji H, Toyoda M, Zhang Y, Narimatsu H, Kuno A
Sci Rep. 2017 Mar 6;7:43560. doi: 10.1038/srep43560

Developmental Research, Epigenetics & Aging

Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons
Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod RJ, Dieterich DC, Blum R, Sendtner M
J Cell Biol. 2017 Feb 28. pii: jcb.201604117. doi: 10.1083/jcb.201604117.

Severe preeclampsia is associated with alterations in cytotrophoblasts of the smooth chorion
Garrido-Gomez T, Ona K, Kapidzic M, Gormley M, Simón C, Genbacev O, Fisher SJ
Development. 2017 Feb 23. pii: dev.146100. doi: 10.1242/dev.146100.

Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis
Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z
Journal of Genetics and Genomics, (2017), doi: 10.1016/j.jgg.2016.12.007

Plant Research

Transcriptome analysis of the Brassica napus - Leptosphaeria maculans pathosystem identifies receptor, signalling and structural genes underlying plant resistance
Becker MG, Zhang X, Walker PL, Wan JC, Millar JL, Khan D, Granger MJ, Cavers JD, Chan AC, Fernando DW, Belmonte MF
Plant J. 2017 Feb 21. doi: 10.1111/tpj.13514.

Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells
Cifuentes M, Baeza V, Arrabal PM, Visser R, Grondona JM, Saldivia N, Martínez F, Nualart F, Salazar K
Mol Neurobiol. 2017 Feb 13. doi: 10.1007/s12035-017-0434-5.

Cell Wall Remodeling In Abscission Zone Cells During Ethylene-Promoted Fruit Abscission In Citrus
Merelo P, Agusti J, Arbona V, Costa ML, Estornell LH, Gomez-Cadenas A, Coimbra SV, Gomez MD, Perez-Amador MA, Domingo C, Talon M, and Tadeo FR
Front. Plant Sci. | doi: 10.3389/fpls.2017.00126

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Surfactant protein D regulates murine testicular immune milieu and sperm functions
Rokade S, Kishore U, Madan T
Am J Reprod Immunol. 2017 Jan 5. doi: 10.1111/aji.12629.

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp
Phan JL, Tucker MR, Khor SF, Shirley N, Lahnstein J, Beahan C, Bacic A, Burton RA
J Exp Bot. 2016 Dec;67(22):6481-6495.

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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