Laser Microdissection Publication List

July 12, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Heterogeneous DNA methylation status in same-cell subpopulations of ovarian cancer tissues
Li Q, Xue X, Li W, Wang Q, Han L, Brunson T, Xu W, Chambers-Harris I, Wang Q, Li X, Ma L, Song Q
Tumour Biol. 2017 Jun;39(6):1010428317701650. doi: 10.1177/1010428317701650.

Variability of DNA Methylation within Schizophrenia Risk Loci across Subregions of Human Hippocampus
Ruzicka WB, Subburaju S, Benes FM
Genes (Basel). 2017 May 15;8(5). pii: E143. doi: 10.3390/genes8050143.

Cancer-Associated Mutations in Endometriosis without Cancer
Anglesio MS, Papadopoulos N, Ayhan A, Nazeran TM, Noë M, Horlings HM, Lum A, Jones S, Senz J, Seckin T, Ho J, Wu RC, Lac V, Ogawa H, Tessier-Cloutier B, Alhassan R, Wang A, Wang Y, Cohen JD, Wong F, Hasanovic A, Orr N, Zhang M, Popoli M, McMahon W, Wood LD, Mattox A, Allaire C, Segars J, Williams C, Tomasetti C, Boyd N, Kinzler KW, Gilks CB, Diaz L, Wang TL, Vogelstein B, Yong PJ, Huntsman DG, Shih IM
N Engl J Med. 2017 May 11;376(19):1835-1848. doi: 10.1056/NEJMoa1614814.

RNA Research

Intestinal cancer progression by mutant p53 through the acquisition of invasiveness associated with complex glandular formation
Nakayama M, Sakai E, Echizen K, Yamada Y, Oshima H, Han TS, Ohki R, Fujii S, Ochiai A, Robine S, Voon DC, Tanaka T, Taketo MM, Oshima M
Oncogene. 2017 Jun 19. doi: 10.1038/onc.2017.194.

Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization 
Credle JJ, Itoh CY, Yaun T, Sharma R, Scott ER, Workman RE, Fan Y, Housseau F, Llosa NJ, Bell WR, Miller H, Zhang SX, Timp W, and Larman HB
Nucleic Acids Res 2017 gkx471. doi: 10.1093/nar/gkx471

Dual Regulation of Gluconeogenesis by Insulin and Glucose in the Proximal Tubules of the Kidney
Sasaki M, Sasako T, Kubota N, Sakurai Y, Takamoto I, Kubota T, Inagi R, Seki G, Goto M, Ueki K, Nangaku M, Jomori T, Kadowaki T
Diabetes. 2017 Jun 19. pii: db161602. doi: 10.2337/db16-1602.

Proteomics

Assessment of tumor characteristics based on glycoform analysis of membrane-tethered MUC1
Matsuda A, Higashi M, Nakagawa T, Yokoyama S, Kuno A, Yonezawa S, Narimatsu H
Lab Invest. 2017 Jun 5. doi: 10.1038/labinvest.2017.53.

Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC-MS/MS
Xiao CJ, Liu YC, Luo SH, Hua J, Liu Y, Li SH
Phytochem Anal. 2017 May 9. doi: 10.1002/pca.2687.

Sushi repeat-containing protein 1: a novel disease-associated molecule in cerebral amyloid angiopathy
Inoue Y, Ueda M, Tasaki M, Takeshima A, Nagatoshi A, Masuda T, Misumi Y, Kosaka T, Nomura T, Mizukami M, Matsumoto S, Yamashita T, Takahashi H, Kakita A, Ando Y
Acta Neuropathol. 2017 May 6. doi: 10.1007/s00401-017-1720-z.

Fluorescence Applications

Participation of Tumor-Associated Myeloid Cells in Progression of Amelanotic Melanoma (RMM Tumor Line) in F344 Rats, with Particular Reference to MHC Class II- and CD163-Expressing Cells
Bondoc A, Golbar HM, Pervin M, Katou-Ichikawa C, Tanaka M, Izawa T, Kuwamura M, Yamate J
Cancer Microenviron. 2017 Jun 16. doi: 10.1007/s12307-017-0193-x.

Tissue based metabolite profiling and qualitative comparison of two species of Achyranthes roots by use of UHPLC-QTOF MS and laser micro dissection
Jaiswal Y, Liang Z, Ho A, Chen H, Williams L, Zhao Z
Journal of Pharmaceutical Analysis, 16 June 2017

The let-7/lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury
McDaniel K, Huang L, Sato K, Wu N, Annable T, Zhou T, Ramos-Lorenzo S, Wan Y, Huang Q, Francis H, Glaser S, Tsukamoto H, Alpini G, Meng F
J Biol Chem. 2017 May 23. pii: jbc.M116.773291. doi: 10.1074/jbc.M116.773291.

Cancer Research

Intestinal cancer progression by mutant p53 through the acquisition of invasiveness associated with complex glandular formation
Nakayama M, Sakai E, Echizen K, Yamada Y, Oshima H, Han TS, Ohki R, Fujii S, Ochiai A, Robine S, Voon DC, Tanaka T, Taketo MM, Oshima M
Oncogene. 2017 Jun 19. doi: 10.1038/onc.2017.194.

Heterogeneous DNA methylation status in same-cell subpopulations of ovarian cancer tissues
Li Q, Xue X, Li W, Wang Q, Han L, Brunson T, Xu W, Chambers-Harris I, Wang Q, Li X, Ma L, Song Q
Tumour Biol. 2017 Jun;39(6):1010428317701650. doi: 10.1177/1010428317701650.

Characterization of MicroRNA-200 pathway in ovarian cancer and serous intraepithelial carcinoma of fallopian tube
Yang J, Zhou Y, Ng SK, Huang KC, Ni X, Choi PW, Hasselblatt K, Muto MG, Welch WR, Berkowitz RS, Ng SW
BMC Cancer. 2017 Jun 17;17(1):422. doi: 10.1186/s12885-017-3417-z.

Neurosciences

Lower affinity of isradipine for L-type Ca2+ channels during substantia nigra dopamine neuron-like activity: implications for neuroprotection in Parkinson's disease
Ortner NJ, Bock G, Dougalis A, Kharitonova M, Duda J, Hess S, Tuluc P, Pomberger T, Stefanova N, Pitterl F, Ciossek T, Oberacher H, Draheim HJ, Kloppenburg P, Liss B, Striessnig J
J Neurosci. 2017 Jun 7. pii: 2946-16. doi: 10.1523/JNEUROSCI.2946-16.2017.

Development of transcripts regulating dendritic spines in layer 3 pyramidal cells of the monkey prefrontal cortex: Implications for the pathogenesis of schizophrenia
Dienel SJ, Bazmi HH, Lewis DA
Neurobiol Dis. 2017 May 30. pii: S0969-9961(17)30125-0. doi: 10.1016/j.nbd.2017.05.016.

Variability of DNA Methylation within Schizophrenia Risk Loci across Subregions of Human Hippocampus
Ruzicka WB, Subburaju S, Benes FM
Genes (Basel). 2017 May 15;8(5). pii: E143. doi: 10.3390/genes8050143.

Clinical Applications

Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization 
Credle JJ, Itoh CY, Yaun T, Sharma R, Scott ER, Workman RE, Fan Y, Housseau F, Llosa NJ, Bell WR, Miller H, Zhang SX, Timp W, and Larman HB
Nucleic Acids Res 2017 gkx471. doi: 10.1093/nar/gkx471

Dual Regulation of Gluconeogenesis by Insulin and Glucose in the Proximal Tubules of the Kidney
Sasaki M, Sasako T, Kubota N, Sakurai Y, Takamoto I, Kubota T, Inagi R, Seki G, Goto M, Ueki K, Nangaku M, Jomori T, Kadowaki T
Diabetes. 2017 Jun 19. pii: db161602. doi: 10.2337/db16-1602.

TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Kim JB, Pjanic M, Nguyen T, Miller CL, Iyer D, Liu B, Wang T, Sazonova O, Carcamo-Orive I, Matic LP, Maegdefessel L, Hedin U, Quertermous T
PLoS Genet. 2017 May 8;13(5):e1006750. doi: 10.1371/journal.pgen.1006750.

Developmental Research, Epigenetics & Aging

Expression of the matrix gla protein (MGP) gene in different developmental stages of mouse mandibular first molar root
Wang R, Dang, P, Niu Z
Biomedical Research, Jun 2017

The effects of IGF-1 on mouse spermatogenesis using an organ culture method
Yao J, Zuo H, Gao J, Wang M, Wang D, Li X
Biochem Biophys Res Commun. 2017 May 25. pii: S0006-291X(17)31018-5.

Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics
Verckist L, Lembrechts R, Thys S, Pintelon I, Timmermans JP, Brouns I, Adriaensen D
Respir Res. 2017 May 8;18(1):87. doi: 10.1186/s12931-017-0571-4.

Plant Research

Tissue based metabolite profiling and qualitative comparison of two species of Achyranthes roots by use of UHPLC-QTOF MS and laser micro dissection
Jaiswal Y, Liang Z, Ho A, Chen H, Williams L, Zhao Z
Journal of Pharmaceutical Analysis, 16 June 2017

Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC-MS/MS
Xiao CJ, Liu YC, Luo SH, Hua J, Liu Y, Li SH
Phytochem Anal. 2017 May 9. doi: 10.1002/pca.2687.

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Crenothrix are major methane consumers in stratified lakes
Oswald K, Graf JS, Littmann S, Tienken D, Brand A, Wehrli B, Albertsen M, Daims H, Wagner M, Kuypers MM, Schubert CJ, Milucka J
ISME J. 2017 Jun 6. doi: 10.1038/ismej.2017.77.

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Motility in liquid and semisolid media of Paenibacillus polymyxa associative rhizobacteria differing in exopolysaccharide yield and properties
Yegorenkova IV, Tregubova KV, Schelud’ko AV
Symbiosis (2017). doi:10.1007/s13199-017-0492-5

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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