DNA Research
The C134W (402 C>G) FOXL2 mutation is absent in ovarian gynandroblastoma: insights into the genesis of an unusual tumour
Oparka R, Cassidy A, Reilly S, Stenhouse A, McCluggage WG, Herrington CS
Histopathology. 2012 Feb 1. doi: 10.1111/j.1365-2559.2011.04148.x.
Absence of the BRAF and the GRIM-19 Mutations in Oncocytic (Hurthle Cell) Solid Cell Nests of the Thyroid
Cameselle-Teijeiro J, Ferreira R, Caramés N, Abdulkader I, Máximo V, Soares P, Sobrinho-Simões M
Am J Clin Pathol. 2012 Apr;137(4):612-8
Disruption of Trp53 in Livers of Mice Induces Formation of Carcinomas with Bilineal Differentiation
Katz SF, Lechel A, Obenauf AC, Begus-Nahrmann Y, Kraus J, Hoffmann EM, Duda J, Eshraghi P, Hartmann D, Liss B, Schirmacher P, Kestler HA, Speicher MR, Rudolph KL
Gastroenterology. 2012 Feb 15. [Epub ahead of print]
RNA Research
Kruppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract
Blackmore MG, Wang Z, Lerch JK, Motti D, Zhang YP, Shields CB, Lee JK, Goldberg JL, Lemmon VP, Bixby JL
Proc Natl Acad Sci U S A. 2012 Apr 23. [Epub ahead of print]
Transcriptional architecture of the primate neocortex
Bernard A, Lubbers LS, Tanis KQ, Luo R, Podtelezhnikov AA, Finney EM, McWhorter MM, Serikawa K, Lemon T, Morgan R, Copeland C, Smith K, Cullen V, Davis-Turak J, Lee CK, Sunkin SM, Loboda AP, Levine DM, Stone DJ, Hawrylycz MJ, Roberts CJ, Jones AR, Geschwind DH, Lein ES
Neuron. 2012 Mar 22;73(6):1083-99.
Anti-inflammatory effect of IL-6 receptor blockade in corneal alkali burn
Sakimoto, T., Sugaya, S., Ishimori, A., and Sawa, M.
Exp Eye Res, Volume 97, Issue 1, April 2012, Pages 98–104
Proteomics
Updates in Cardiac Amyloidosis: A Review
Banypersad, SM; Moon, JC; Whelan, C; Hawkins, PN; Wechalekar, AD
Journal of the American Heart Association. 2012; 1: e000364 doi: 10.1161/JAHA.111.000364
Association of a Novel Complement Factor H Mutation With Severe Crescentic and Necrotizing Glomerulonephritis
Fervenza FC, Smith RJ, Sethi S
Am J Kidney Dis. 2012 Apr 25. [Epub ahead of print]
Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis
Sethi S, Vrana JA, Theis JD, Leung N, Sethi A, Nasr SH, Fervenza FC, Cornell LD, Fidler ME, Dogan A
Kidney Int. 2012 Apr 11. doi: 10.1038/ki.2012.108.
Fluorescence Applications
Molecular Characterisation of Transport Mechanisms at the Developing Mouse Blood-CSF Interface: A Transcriptome Approach
Liddelow SA, Temple S, Møllgård K, Gehwolf R, Wagner A, Bauer H, Bauer HC, Phoenix TN, Dziegielewska KM, Saunders NR
PLoS One. 2012;7(3):e33554. Epub 2012 Mar 21.
IκB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-κB in the central nervous system
Raasch J, Zeller N, van Loo G, Merkler D, Mildner A, Erny D, Knobeloch KP, Bethea JR, Waisman A, Knust M, Del Turco D, Deller T, Blank T, Priller J, Brück W, Pasparakis M, Prinz
MBrain 134(Pt 4): 1184-98 (Epub 2011)
Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis
Sethi S, Vrana JA, Theis JD, Leung N, Sethi A, Nasr SH, Fervenza FC, Cornell LD, Fidler ME, Dogan A
Kidney Int. 2012 Apr 11. doi: 10.1038/ki.2012.108.
Cancer Research
Lymph node-induced immune tolerance in chronic lymphocytic leukaemia: a role for caveolin-1
Gilling CE, Mittal AK, Chaturvedi NK, Iqbal J, Aoun P, Bierman PJ, Bociek RG, Weisenburger DD, Joshi SS
Br J Haematol. 2012 May 10. doi: 10.1111/j.1365-2141.2012.09148.x.
Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic microRNA clusters
Nishida N, Nagahara M, Sato T, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Sugihara K, Doki Y, Mori M
Clin Cancer Res. 2012 Mar 27. [Epub ahead of print]
Beta Defensin-2 Is Reduced in Central but Not in Distal Airways of Smoker COPD Patients
Pace E , Ferraro M , Minervini MI , Vitulo P , Pipitone L, Chiappara G, Siena L, Montalbano AM, Johnson M, Gjomarkaj M
PLoS ONE 7(3): e33601. doi:10.1371/journal.pone.0033601
Neurosciences
Analysis of region-specific changes in gene expression upon treatment with citalopram and desipramine reveals temporal dynamics in response to antidepressant drugs at the transcriptome level
Gaska M, Kusmider M, Solich J, Faron-Górecka A, Krawczyk MJ, Kulakowski K, Dziedzicka-Wasylewska M
Psychopharmacology (Berl). 2012 May 1. [Epub ahead of print]
Transcriptional profiling of layers of the primate cerebral cortex
Molnár Z, Belgard TG
Neuron. 2012 Mar 22;73(6):1053-5.
Short-term regulation of the hypothalamic melanocortinergic system under fasting and defined glucose-refeeding conditions in rats: A lasercapture microdissection (LMD)-based study
Landmann EM, Schellong K, Melchior K, Rodekamp E, Ziska T, Harder T, Plagemann A
Neurosci Lett. 2012 Apr 25;515(1):87-91.
Clinical Applications
Membranoproliferative glomerulonephritis--a new look at an old entity
Sethi S, Fervenza FC
N Engl J Med. 2012 Mar 22;366(12):1119-31
Epidermal growth factor receptor copy number in potentially malignant oral disorders and oral squamous cell carcinoma: a short communication and preliminary study
Bagan JV, Mata-Roig M, Cortio-Gimeno J, Murillo-Cortes J, Hens-Aumente E, Poveda-Roda R, Bagan L
J Oral Pathol Med. 2012 Mar 14. doi: 10.1111/j.1600-0714.2012.01137.x.
Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis
Sethi S, Vrana JA, Theis JD, Leung N, Sethi A, Nasr SH, Fervenza FC, Cornell LD, Fidler ME, Dogan A
Kidney Int. 2012 Apr 11. doi: 10.1038/ki.2012.108.
Developmental Research
Mitochondrial Respiration Is Decreased in Rat Kidney Following Fetal Exposure to a MaternalLow-ProteinDiet
Engeham S, Mdaki K, Jewell K, Austin R, Lehner AN, and Langley-Evans SC
Journal of Nutrition and Metabolism Volume 2012 (2012), Article ID 989037, 10 pages, doi:10.1155/2012/989037
Increased cellular distribution of vimentin and Ret in the cingulum induced by developmental hypothyroidism in rat offspring maternally exposed to anti-thyroid agents
Fujimoto H, Woo GH, Inoue K, Igarashi K, Kanno J, Hirose M, Nishikawa A, and Shibutani M
Reproductive Toxicology, Available online 6 April 2012, ISSN 0890-6238, 10.1016/j.reprotox.2012.03.005.
The nuclear factor-κB pathway is involved in matrix metalloproteinase-9 expression in RU486-induced endometrium breakdown in mice
Li YF, Xu XB, Chen XH, Wei G, He B, Wang JD
Hum Reprod. 2012 May 15.
Plant Research
Sugar transporters in plants and in their interactions with fungi
Doidy J, Grace E, Kühn C, Simon-Plas F, Casieri L, Wipf D
Trends Plant Sci. 2012 Apr 16.
Metabolite analyses of single cells
Oikawa A, Saito K
Plant J. 2012 Apr;70(1):30-8. doi: 10.1111/j.1365-313X.2012.04967.x.
Analysis of plant germline development by high-throughput RNA profiling: technical advances and new insights
Schmidt A, Schmid MW, Grossniklaus U
Plant J. 2012 Apr;70(1):18-29. doi: 10.1111/j.1365-313X.2012.04897.x.
Forensics
DNA mixture genotyping by probabilistic computer interpretation of binomially-sampled laser captured cell populations: Combining quantitative data for greater identification information
Ballantyne J, Hanson EK, Perlin MW
Science & Justice, ISSN 1355-0306, 10.1016/j.scijus.2012.04.004
Development of a one-tube extraction and amplification method for DNA analysis of sperm and epithelial cells recovered from forensic samples by laser microdissection
Meredith M, Bright JA, Cockerton S, Vintiner S
Forensic Sci Int Genet. 2012 Jan;6(1):91-6.
Laser microdissection: Checking that the dissected cells are recovered for DNA extraction
Thorpe S.M., Pedersen N.A., Seddon T.J., Prince D.V., Goucher M.J., van Oorschot R.A.H.
Forensic Science International: Genetics Supplement Series (2011), doi:10.1016/j.fsigss.2011.08.073
Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.
Miscellaneous
Detecting metabolic activities in single cells, with emphasis on nanoSIMS
Musat N, Foster R, Vagner T, Adam B, Kuypers MM
FEMS Microbiol Rev. 2012 Mar;36(2):486-511. doi: 10.1111/j.1574-6976.2011.00303.x.
Look@NanoSIMS - a tool for the analysis of nanoSIMS data in environmental microbiology
Polerecky L, Adam B, Milucka J, Musat N, Vagner T, Kuypers MM
Environ Microbiol. 2012 Apr;14(4):1009-23. doi: 10.1111/j.1462-2920.2011.02681.x.
Common Variants at 9p21 and 8q22 Are Associated with Increased Susceptibility to Optic Nerve Degeneration in Glaucoma
Wiggs JL, Yaspan BL, Hauser MA, Kang JH, Allingham RR, et al.
PLoS Genet 8(4): e1002654. doi:10.1371/journal.pgen.1002654
Living Cell Culture
Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression
Lammers G, Roth G, Heck M, Zengerle R, Tjabringa GS, Versteeg EM, Hafmans T, Wismans R, Reinhardt DP, Verwiel ET, Zeeuwen PL, Schalkwijk J, Brock R, Daamen WF, van Kuppevelt TH
Macromol Biosci. 2012 Mar 13. doi: 10.1002/mabi.201100443. [Epub ahead of print]
Laser microdissection microscopy: application to cell culture
Mustafa A, Cenayko C, Mitry RR, Quaglia A
Methods Mol Biol. 2012;806:385-92.