Laser Microdissection Publication List

December 03, 2016

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

A renewed model of pancreatic cancer evolution based on genomic rearrangement patterns
Notta F, Chan-Seng-Yue M, Lemire M, Li Y, Wilson GW, Connor AA, Denroche RE, Liang SB, Brown AM, Kim JC, Wang T, Simpson JT, Beck T, Borgida A, Buchner N, Chadwick D, Hafezi-Bakhtiari S, Dick JE, Heisler L, Hollingsworth MA, Ibrahimov E, Jang GH, Johns J, Jorgensen LG, Law C, Ludkovski O, Lungu I, Ng K, Pasternack D, Petersen GM, Shlush LI, Timms L, Tsao MS, Wilson JM, Yung CK, Zogopoulos G, Bartlett JM, Alexandrov LB, Real FX, Cleary SP, Roehrl MH, McPherson JD, Stein LD, Hudson TJ, Campbell PJ, Gallinger S
Nature. 2016 Oct 12. doi: 10.1038/nature19823.

Simul-seq: combined DNA and RNA sequencing for whole-genome and transcriptome profiling
Reuter JA, Spacek DV, Pai RK, Snyder MP
Nat Methods. 2016 Oct 10. doi: 10.1038/nmeth.4028.

A Phenotype-Driven Approach to Generate Mouse Models with Pathogenic mtDNA Mutations Causing Mitochondrial Disease
Kauppila JH, Baines HL, Bratic A, Simard ML, Freyer C, Mourier A, Stamp C, Filograna R, Larsson NG, Greaves LC, Stewart JB
Cell Rep. 2016 Sep 13;16(11):2980-90. doi: 10.1016/j.celrep.2016.08.037.

RNA Research

Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling
Martin LB, Nicolas P, Matas AJ, Shinozaki Y, Catalá C, Rose JK
Nat Protoc. 2016 Dec;11(12):2376-2388. doi: 10.1038/nprot.2016.146.

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse
Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L, Mai M, Beckers A, Kracht M, Gossler A
Dev Biol. 2016 Nov 30. pii: S0012-1606(16)30313-X. doi: 10.1016/j.ydbio.2016.11.019.

Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Del Turco D, Paul MH, Schlaudraff J, Hick M, Endres K, Müller UC, and Deller T
Front. Mol. Neurosci., 29 November 2016

Proteomics

Diagnosis, pathogenesis and outcome in leucocyte chemotactic factor 2 (ALECT2) amyloidosis
Rezk T, Gilbertson JA, Rowczenio D, Bass P, Lachmann HJ, Wechalekar AD, Fontana M, Mahmood S, Sachchithanantham S, Whelan CJ, Wong J, Rendell N, Taylor GW, Hawkins PN, and Gillmore JD
Nephrol. Dial. Transplant. (2016) doi: 10.1093/ndt/gfw375

Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Del Turco D, Paul MH, Schlaudraff J, Hick M, Endres K, Müller UC, and Deller T
Front. Mol. Neurosci., 29 November 2016

Renal amyloidosis associated with five novel variants in the fibrinogen A-alpha chain protein
Rowczenio D, Stensland M, de Souza GA, Strøm EH, Gilbertson JA, Taylor G, Rendell N, Minogue S, Efebera YA, Lachmann HJ, Wechalekar AD, Hawkins PN, Heimdal KR, Selvig K, Lægreid IK, Gillmore JD, Wien TN
Kidney International Reports (2016), doi: 10.1016/j.ekir.2016.11.005.

Fluorescence Applications

Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'
Nakashima Y, Egami Y, Kimura M, Wakimoto T, Abe I
PLoS One. 2016 Oct 12;11(10):e0164468. doi: 10.1371/journal.pone.0164468. eCollection 2016.

Tracking the route of phenanthrene uptake in mosses: An experimental trial
Spagnuolo V, Figlioli F, De Nicola F, Capozzi F, Giordano S
Sci Total Environ. 2016 Sep 29. pii: S0048-9697(16)32104-0. doi: 10.1016/j.scitotenv.2016.09.174.

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Cancer Research

Gasdermin C Is Upregulated by Inactivation of Transforming Growth Factor β Receptor Type II in the Presence of Mutated Apc, Promoting Colorectal Cancer Proliferation
Miguchi M, Hinoi T, Shimomura M, Adachi T, Saito Y, Niitsu H, Kochi M, Sada H, Sotomaru Y, Ikenoue T, Shigeyasu K, Tanakaya K, Kitadai Y, Sentani K, Oue N, Yasui W, Ohdan H
PLoS One. 2016 Nov 11;11(11):e0166422. doi: 10.1371/journal.pone.0166422. eCollection 2016.

The expression pattern of matrix-producing tumor stroma is of prognostic importance in breast cancer
Winslow S, Lindquist KE, Edsjö A, Larsson C
BMC Cancer. 2016 Nov 4;16(1):841

A renewed model of pancreatic cancer evolution based on genomic rearrangement patterns
Notta F, Chan-Seng-Yue M, Lemire M, Li Y, Wilson GW, Connor AA, Denroche RE, Liang SB, Brown AM, Kim JC, Wang T, Simpson JT, Beck T, Borgida A, Buchner N, Chadwick D, Hafezi-Bakhtiari S, Dick JE, Heisler L, Hollingsworth MA, Ibrahimov E, Jang GH, Johns J, Jorgensen LG, Law C, Ludkovski O, Lungu I, Ng K, Pasternack D, Petersen GM, Shlush LI, Timms L, Tsao MS, Wilson JM, Yung CK, Zogopoulos G, Bartlett JM, Alexandrov LB, Real FX, Cleary SP, Roehrl MH, McPherson JD, Stein LD, Hudson TJ, Campbell PJ, Gallinger S
Nature. 2016 Oct 12. doi: 10.1038/nature19823.

Neurosciences

Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Del Turco D, Paul MH, Schlaudraff J, Hick M, Endres K, Müller UC, and Deller T
Front. Mol. Neurosci., 29 November 2016

Stimulation of the Hippocampal POMC/MC4R Circuit Alleviates Synaptic Plasticity Impairment in an Alzheimer's Disease Model
Shen Y, Tian M, Zheng Y, Gong F, Fu AK, Ip NY
Cell Rep. 2016 Nov 8;17(7):1819-1831. doi: 10.1016/j.celrep.2016.10.043.

Early lipofuscin accumulation in Frontal Lobe Epilepsy
Liu JY, Reeves C, Diehl B, Coppola A, Al-Hajri A, Hoskote C, Al Mughairy S, Tachrount M, Groves M, Michalak Z, Mills K, McEvoy AW, Miserocchi A, Sisodiya SM, Thom M
Ann Neurol. 2016 Oct 20. doi: 10.1002/ana.24803

Clinical Applications

Diagnosis, pathogenesis and outcome in leucocyte chemotactic factor 2 (ALECT2) amyloidosis
Rezk T, Gilbertson JA, Rowczenio D, Bass P, Lachmann HJ, Wechalekar AD, Fontana M, Mahmood S, Sachchithanantham S, Whelan CJ, Wong J, Rendell N, Taylor GW, Hawkins PN, and Gillmore JD
Nephrol. Dial. Transplant. (2016) doi: 10.1093/ndt/gfw375

Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
Vidaña B, Martínez J, Martorell J, Montoya M, Córdoba L, Pérez M, Majó N
Vet Res. 2016 Nov 8;47(1):113.

PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation
Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barabási AL, Boothby M, Aikawa E, Singh SA, Aikawa M
Nat Commun. 2016 Oct 31;7:12849. doi: 10.1038/ncomms12849.

Developmental Research, Epigenetics & Aging

Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse
Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L, Mai M, Beckers A, Kracht M, Gossler A
Dev Biol. 2016 Nov 30. pii: S0012-1606(16)30313-X. doi: 10.1016/j.ydbio.2016.11.019.

The Impact of Cell Surface PEGylation and Short-Course Immunotherapy on Islet Graft Survival in an Allogeneic Murine Model
Giraldo JA, Damaris Molano R, Rengifo HR, Fotino C, Gattás-Asfura KM, Pileggi A, Stabler CL
Acta Biomater. 2016 Nov 30. pii: S1742-7061(16)30656-0. doi: 10.1016/j.actbio.2016.11.060.

Transcriptomic Variation during Spermiogenesis in Mouse Germ Cells
Zuo H, Zhang J, Zhang L, Ren X, Chen X, Hao H, Zhao X, Wang D
PLoS One. 2016 Nov 11;11(11):e0164874. doi: 10.1371/journal.pone.0164874. eCollection 2016.

Plant Research

Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling
Martin LB, Nicolas P, Matas AJ, Shinozaki Y, Catalá C, Rose JK
Nat Protoc. 2016 Dec;11(12):2376-2388. doi: 10.1038/nprot.2016.146.

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp
Phan JL, Tucker MR, Khor SF, Shirley N, Lahnstein J, Beahan C, Bacic A, Burton RA
J Exp Bot. 2016 Dec;67(22):6481-6495.

Fungal and plant gene expression in the Tulasnella calospora-Serapias vomeracea symbiosis provides clues about nitrogen pathways in orchid mycorrhizas
Fochi V, Chitarra W, Kohler A, Voyron S, Singan VR, Lindquist EA, Barry KW, Girlanda M, Grigoriev IV, Martin F, Balestrini R, Perotto S
New Phytol. 2016 Nov 11. doi: 10.1111/nph.14279.

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp
Phan JL, Tucker MR, Khor SF, Shirley N, Lahnstein J, Beahan C, Bacic A, Burton RA
J Exp Bot. 2016 Dec;67(22):6481-6495.

Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'
Nakashima Y, Egami Y, Kimura M, Wakimoto T, Abe I
PLoS One. 2016 Oct 12;11(10):e0164468. doi: 10.1371/journal.pone.0164468. eCollection 2016.

Altered contractile responses of arteries from spontaneously hypertensive rat: The role of endogenous mediators and membrane depolarization
Bencze M, Behuliak M, Vavřínová A, Zicha J
Life Sci. 2016 Oct 6. pii: S0024-3205(16)30587-2. doi: 10.1016/j.lfs.2016.10.005.

Live Cell Culture

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

Altered Transport Velocity of Axonal Mitochondria in Retinal Ganglion Cells After Laser-Induced Axonal Injury In Vitro
Yokota S, Takihara Y, Arimura S, Miyake S, Takamura Y, Yoshimura N, Inatani M
Invest Ophthalmol Vis Sci. 2015 Dec 1;56(13):8019-8025. doi: 10.1167/iovs.15-17876.

Isolation of single Chlamydia-infected cells using laser microdissection
Podgorny OV, Polina NF, Babenko VV, Karpova IY, Kostryukova ES, Govorun VM, Lazarev VN
J Microbiol Methods. 2014 Dec 26. pii: S0167-7012(14)00367-4. doi: 10.1016/j.mimet.2014.12.018.

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