Laser Microdissection Publication List

April 27, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture microdissection and cycling temperature capillary electrophoresis
Refinetti P, Arstad C, Thilly WG, Morgenthaler S, Ekstrøm PO
BMC Clinical Pathology (2017) 17:6 DOI 10.1186/s12907-017-0042-3

Development of bioluminescent chick chorioallantoic membrane (CAM) models for primary pancreatic cancer cells: a platform for drug testing
Rovithi M, Avan A, Funel N, Leon LG, Gomez VE, Wurdinger T, Griffioen AW, Verheul HM, Giovannetti E
Sci Rep. 2017 Mar 17;7:44686. doi: 10.1038/srep44686.

Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection
Ma L, Li W, Song Q
Methods Mol Biol. 2017;1551:161-169. doi: 10.1007/978-1-4939-6750-6_9.

RNA Research

Local Inflammatory Cues Regulate Differentiation and Persistence of CD8+ Tissue-Resident Memory T Cells
Bergsbaken T1, Bevan MJ2, Fink PJ
Cell Rep. 2017 Apr 4;19(1):114-124. doi: 10.1016/j.celrep.2017.03.031.

Immune hyperreactivity of Aβ plaque-associated microglia in Alzheimer’s disease
Yin Z, Raj D, Saiepour N, Van Dam D, Brouwer N, Holtman IR, Eggen BJL, Möller T, Tamm JA, Abdourahman A, Hol EM, Kamphuis W, Bayer TA, DeDeyn PP, Boddeke E
Neurobiology of Aging, 27 Mar 2017, ISSN 0197-4580, http://dx.doi.org/10.1016/j.neurobiolaging.2017.03.021.

Identification of Intrinsic Axon Growth Modulators for Intact CNS Neurons after Injury
Fink KL, López-Giráldez F, Kim IJ, Strittmatter SM, Cafferty WB
Cell Rep. 2017 Mar 14;18(11):2687-2701. doi: 10.1016/j.celrep.2017.02.058.

Proteomics

LMD proteomics provides evidence for hippocampus field-specific motor protein abundance changes with relevance to Alzheimer's disease
Schrötter A, Oberhaus A, Kolbe K, Seger S, Mastalski T, El Magraoui F, Hoffmann-Posorske E, Bohnert M, Deckert J, Braun C, Graw M, Schmitz C, Arzberger T, Loosse C, Heinsen H, Meyer HE, Müller T
Biochim Biophys Acta. 2017 Apr 1. pii: S1570-9639(17)30066-3. doi: 10.1016/j.bbapap.2017.03.013.

Spatial and molecular resolution of diffuse malignant mesothelioma heterogeneity by integrating label-free FTIR imaging, laser capture microdissection and proteomics
Großerueschkamp F, Bracht T, Diehl HC, Kuepper C, Ahrens M, Kallenbach-Thieltges A, Mosig A, Eisenacher M, Marcus K, Behrens T, Brüning T, Theegarten D, Sitek B, Gerwert K
Sci Rep. 2017 Mar 30;7:44829. doi: 10.1038/srep44829.

Proteomic differences in amyloid plaques in rapidly progressive and sporadic Alzheimer's disease
Drummond E, Nayak S, Faustin A, Pires G, Hickman RA, Askenazi M, Cohen M, Haldiman T, Kim C, Han X, Shao Y, Safar JG, Ueberheide B, Wisniewski T
Acta Neuropathol. 2017 Mar 4. doi: 10.1007/s00401-017-1691-0.

Fluorescence Applications

Local Inflammatory Cues Regulate Differentiation and Persistence of CD8+ Tissue-Resident Memory T Cells
Bergsbaken T1, Bevan MJ2, Fink PJ
Cell Rep. 2017 Apr 4;19(1):114-124. doi: 10.1016/j.celrep.2017.03.031.

The NF-κB-dependent and -independent transcriptome and chromatin landscapes of human coronavirus 229E-infected cells
Poppe M, Wittig S, Jurida L, Bartkuhn M, Wilhelm J, Müller H, Beuerlein K, Karl N, Bhuju S, Ziebuhr J, Schmitz ML, Kracht M
PLoS Pathog. 2017 Mar 29;13(3):e1006286. doi: 10.1371/journal.ppat.1006286.

Identification of Intrinsic Axon Growth Modulators for Intact CNS Neurons after Injury
Fink KL, López-Giráldez F, Kim IJ, Strittmatter SM, Cafferty WB
Cell Rep. 2017 Mar 14;18(11):2687-2701. doi: 10.1016/j.celrep.2017.02.058.

Cancer Research

Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture microdissection and cycling temperature capillary electrophoresis
Refinetti P, Arstad C, Thilly WG, Morgenthaler S, Ekstrøm PO
BMC Clinical Pathology (2017) 17:6 DOI 10.1186/s12907-017-0042-3

Spatial and molecular resolution of diffuse malignant mesothelioma heterogeneity by integrating label-free FTIR imaging, laser capture microdissection and proteomics
Großerueschkamp F, Bracht T, Diehl HC, Kuepper C, Ahrens M, Kallenbach-Thieltges A, Mosig A, Eisenacher M, Marcus K, Behrens T, Brüning T, Theegarten D, Sitek B, Gerwert K
Sci Rep. 2017 Mar 30;7:44829. doi: 10.1038/srep44829.

Development of bioluminescent chick chorioallantoic membrane (CAM) models for primary pancreatic cancer cells: a platform for drug testing
Rovithi M, Avan A, Funel N, Leon LG, Gomez VE, Wurdinger T, Griffioen AW, Verheul HM, Giovannetti E
Sci Rep. 2017 Mar 17;7:44686. doi: 10.1038/srep44686.

Neurosciences

LMD proteomics provides evidence for hippocampus field-specific motor protein abundance changes with relevance to Alzheimer's disease
Schrötter A, Oberhaus A, Kolbe K, Seger S, Mastalski T, El Magraoui F, Hoffmann-Posorske E, Bohnert M, Deckert J, Braun C, Graw M, Schmitz C, Arzberger T, Loosse C, Heinsen H, Meyer HE, Müller T
Biochim Biophys Acta. 2017 Apr 1. pii: S1570-9639(17)30066-3. doi: 10.1016/j.bbapap.2017.03.013.

Identification of Intrinsic Axon Growth Modulators for Intact CNS Neurons after Injury
Fink KL, López-Giráldez F, Kim IJ, Strittmatter SM, Cafferty WB
Cell Rep. 2017 Mar 14;18(11):2687-2701. doi: 10.1016/j.celrep.2017.02.058.

Proteomic differences in amyloid plaques in rapidly progressive and sporadic Alzheimer's disease
Drummond E, Nayak S, Faustin A, Pires G, Hickman RA, Askenazi M, Cohen M, Haldiman T, Kim C, Han X, Shao Y, Safar JG, Ueberheide B, Wisniewski T
Acta Neuropathol. 2017 Mar 4. doi: 10.1007/s00401-017-1691-0.

Clinical Applications

Local Inflammatory Cues Regulate Differentiation and Persistence of CD8+ Tissue-Resident Memory T Cells
Bergsbaken T1, Bevan MJ2, Fink PJ
Cell Rep. 2017 Apr 4;19(1):114-124. doi: 10.1016/j.celrep.2017.03.031.

The NF-κB-dependent and -independent transcriptome and chromatin landscapes of human coronavirus 229E-infected cells
Poppe M, Wittig S, Jurida L, Bartkuhn M, Wilhelm J, Müller H, Beuerlein K, Karl N, Bhuju S, Ziebuhr J, Schmitz ML, Kracht M
PLoS Pathog. 2017 Mar 29;13(3):e1006286. doi: 10.1371/journal.ppat.1006286.

Preeclampsia: Novel Insights from Global RNA Profiling of Trophoblast Subpopulations
Gormley M, Ona K, Kapidzic M, Garrido-Gomez T, Zdravkovic T, Fisher SJ
Am J Obstet Gynecol. 2017 Mar 24. pii: S0002-9378(17)30441-6. doi: 10.1016/j.ajog.2017.03.017.

Developmental Research, Epigenetics & Aging

Preeclampsia: Novel Insights from Global RNA Profiling of Trophoblast Subpopulations
Gormley M, Ona K, Kapidzic M, Garrido-Gomez T, Zdravkovic T, Fisher SJ
Am J Obstet Gynecol. 2017 Mar 24. pii: S0002-9378(17)30441-6. doi: 10.1016/j.ajog.2017.03.017.

Expression and trafficking of placental microRNAs at the feto-maternal interface
Chang G, Mouillet JF, Mishima T, Chu T, Sadovsky E, Coyne CB, Parks WT, Surti U, Sadovsky Y
FASEB J. 2017 Mar 13. pii: fj.201601146R. doi: 10.1096/fj.201601146R.

Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons
Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod RJ, Dieterich DC, Blum R, Sendtner M
J Cell Biol. 2017 Feb 28. pii: jcb.201604117. doi: 10.1083/jcb.201604117.

Plant Research

Control of secondary cell wall patterning involves xylan deacetylation by a GDSL esterase
Zhang B, Zhang L, Li F, Zhang D, Liu X, Wang H, Xu Z, Chu C, Zhou Y
Nat Plants. 2017 Mar 3;3:17017. doi: 10.1038/nplants.2017.17.

Transcriptome analysis of the Brassica napus - Leptosphaeria maculans pathosystem identifies receptor, signalling and structural genes underlying plant resistance
Becker MG, Zhang X, Walker PL, Wan JC, Millar JL, Khan D, Granger MJ, Cavers JD, Chan AC, Fernando DW, Belmonte MF
Plant J. 2017 Feb 21. doi: 10.1111/tpj.13514.

Cell Wall Remodeling In Abscission Zone Cells During Ethylene-Promoted Fruit Abscission In Citrus
Merelo P, Agusti J, Arbona V, Costa ML, Estornell LH, Gomez-Cadenas A, Coimbra SV, Gomez MD, Perez-Amador MA, Domingo C, Talon M, and Tadeo FR
Front. Plant Sci. | doi: 10.3389/fpls.2017.00126

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Targeted Ablation Using Laser Nanosurgery
Gayathri Vegesna NV, Ronchi P, Durdu S, Terjung S, Pepperkok R
Methods Mol Biol. 2017;1563:107-125. doi: 10.1007/978-1-4939-6810-7_8

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Surfactant protein D regulates murine testicular immune milieu and sperm functions
Rokade S, Kishore U, Madan T
Am J Reprod Immunol. 2017 Jan 5. doi: 10.1111/aji.12629.

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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