Laser Microdissection Publication List

September 12, 2016

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

A Phenotype-Driven Approach to Generate Mouse Models with Pathogenic mtDNA Mutations Causing Mitochondrial Disease
Kauppila JH, Baines HL, Bratic A, Simard ML, Freyer C, Mourier A, Stamp C, Filograna R, Larsson NG, Greaves LC, Stewart JB
Cell Rep. 2016 Sep 13;16(11):2980-90. doi: 10.1016/j.celrep.2016.08.037.

Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum
Zhou FC, Resendiz M, Lo CL, Chen Y
PLoS One. 2016 Sep 1;11(9):e0162063. doi: 10.1371/journal.pone.0162063. eCollection 2016.

Latent mitochondrial DNA deletion mutations drive muscle fiber loss at old age
Herbst A, Wanagat J, Cheema N, Widjaja K, McKenzie D, Aiken JM
Aging Cell. 2016 Aug 25. doi: 10.1111/acel.12520.

RNA Research

Epigenomic and metabolic responses of hypothalamic POMC neurons to gestational nicotine exposure in adult offspring
Silva JP, Lambert G, van Booven D, Wahlestedt C
Genome Med. 2016 Sep 8;8(1):93. doi: 10.1186/s13073-016-0348-2.

Novel microRNA revealed by systematic analysis of the microRNA transcriptome in dentate gyrus granule cells
Ryan B, Williams JM
Neurosci Lett. 2016 Sep 6. pii: S0304-3940(16)30664-4. doi: 10.1016/j.neulet.2016.09.003.

Identification of marker genes for pars tuberalis morphogenesis in chick embryo: expression of Cytokine-like 1 and Gap junction protein alpha 5 in pars tuberalis
Aizawa S, Higaki Y, Dudaui A, Nagasaka M, Takahashi S, Sakata I, Sakai T
Cell Tissue Res. 2016 Sep 3.

Proteomics

Characterization of C3 in C3 glomerulopathy
Sethi S, Vrana JA, Fervenza FC, Theis JD, Sethi A, Kurtin PJ, Zhang Y, Smith RJ
Nephrol Dial Transplant. 2016 Aug 8. pii: gfw290.

2A4 binds soluble and insoluble light chain aggregates from AL amyloidosis patients and promotes clearance of amyloid deposits by phagocytosis †
Renz M, Torres R, Dolan PJ, Tam SJ, Tapia JR, Li L, Salmans JR, Barbour RM, Shughrue PJ, Nijjar T, Schenk D, Kinney GG, Zago W
Amyloid. 2016 Aug 5:1-10.

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Fluorescence Applications

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Mutant desmin substantially perturbs mitochondrial morphology, function and maintenance in skeletal muscle tissue
Winter L, Wittig I, Peeva V, Eggers B, Heidler J, Chevessier F, Kley RA, Barkovits K, Strecker V, Berwanger C, Herrmann H, Marcus K, Kornblum C, Kunz WS, Schröder R, Clemen CS
Acta Neuropathol. 2016 Jul 8.

α2A adrenergic receptors highly expressed in mesoprefrontal dopamine neurons
Castelli MP, Spiga S, Perra A, Madeddu C, Mulas G, Ennas MG, Gessa GL
Neuroscience. 2016 Jun 27. pii: S0306-4522(16)30276-7. doi: 10.1016/j.neuroscience.2016.06.037.

Cancer Research

The long noncoding RNA H19 promotes cell proliferation via E2F-1 in pancreatic ductal adenocarcinoma
Ma L, Tian X, Wang F, Zhang Z, Du C, Xie X, Kornmann M, Yang Y
Cancer Biol Ther. 2016 Aug 29:1-11.

BCL9L expression in pancreatic neoplasia with a focus on SPN: a possible explanation for the enigma of the benign neoplasia
Hallas C, Phillipp J, Domanowsky L, Kah B, Tiemann K
BMC Cancer. 2016 Aug 18;16:648. doi: 10.1186/s12885-016-2707-1.

Methylation of the SEPT9_v2 promoter as a novel marker for the detection of circulating tumor DNA in breast cancer patients
Matsui S, Kagara N, Mishima C, Naoi Y, Shimoda M, Shimomura A, Shimazu K, Kim SJ, Noguchi S
Oncol Rep. 2016 Aug 4. doi: 10.3892/or.2016.5004.

Neurosciences

Meta-Analysis of Parkinson's Disease Transcriptome Data Using TRAM Software: Whole Substantia Nigra Tissue and Single Dopamine Neuron Differential Gene Expression
Mariani E, Frabetti F, Tarozzi A, Pelleri MC, Pizzetti F, Casadei R
PLoS One. 2016 Sep 9;11(9):e0161567. doi: 10.1371/journal.pone.0161567. eCollection 2016.

Epigenomic and metabolic responses of hypothalamic POMC neurons to gestational nicotine exposure in adult offspring
Silva JP, Lambert G, van Booven D, Wahlestedt C
Genome Med. 2016 Sep 8;8(1):93. doi: 10.1186/s13073-016-0348-2.

Novel microRNA revealed by systematic analysis of the microRNA transcriptome in dentate gyrus granule cells
Ryan B, Williams JM
Neurosci Lett. 2016 Sep 6. pii: S0304-3940(16)30664-4. doi: 10.1016/j.neulet.2016.09.003.

Clinical Applications

Exploring the Potential of Laser Capture Microdissection Technology in Integrated Oral BioSciences
Thennavan A, Sharma M, Chandrashekar C, Hunter K, Radhakrishnan R
Oral Dis. 2016 Aug 31. doi: 10.1111/odi.12578.

Characterization of C3 in C3 glomerulopathy
Sethi S, Vrana JA, Fervenza FC, Theis JD, Sethi A, Kurtin PJ, Zhang Y, Smith RJ
Nephrol Dial Transplant. 2016 Aug 8. pii: gfw290.

Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Ganglia of Human Gastrointestinal Tract
Xue R, Gu H, Qiu Y, Guo Y, Korteweg C, Huang J, Gu J
Sci Rep. 2016 Aug 5;6:30926. doi: 10.1038/srep30926.

Developmental Research, Epigenetics & Aging

Identification of marker genes for pars tuberalis morphogenesis in chick embryo: expression of Cytokine-like 1 and Gap junction protein alpha 5 in pars tuberalis
Aizawa S, Higaki Y, Dudaui A, Nagasaka M, Takahashi S, Sakata I, Sakai T
Cell Tissue Res. 2016 Sep 3.

Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum
Zhou FC, Resendiz M, Lo CL, Chen Y
PLoS One. 2016 Sep 1;11(9):e0162063. doi: 10.1371/journal.pone.0162063. eCollection 2016.

GLI1+ progenitor cells in the adrenal capsule of the adult mouse give rise to heterotopic gonadal-like tissue
Dörner J, Martinez Rodriguez V, Ziegler R, Röhrig T, Cochran RS, Götz RM, Levin MD, Pihlajoki M, Heikinheimo M, Wilson DB
Mol Cell Endocrinol. 2016 Aug 29. pii: S0303-7207(16)30350-1. doi: 10.1016/j.mce.2016.08.043.

Plant Research

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Endoplasmic Reticulum Body–Related Gene Expression in Different Root Zones of Arabidopsis Isolated by Laser-Assisted Microdissection
Gotté M, Bérnard M, Kiefer-Meyer MC, Jaber R, Moore JP, Vicré-Gibouin M, and Driouich A
The Plant Genome, Vol. 9 No. 2 Jul 7 2016, doi:10.3835/plantgenome2015.08.0076

Selection and testing of reference genes for accurate RT-qPCR in adult needles and seedlings of maritime pine
Granados JM, Ávila C, Cánovas FM, Cañas RA
Tree Genetics & Genomes, June 2016, 12:60

Forensics

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

STR profiling of epithelial cells identified by X/Y-FISH labelling and laser microdissection using standard and elevated PCR conditions
Lynch L, Gamblin A, Vintiner S, Simons JL
Forensic Sci Int Genet. 2014 Oct 25;16C:1-7. doi: 10.1016/j.fsigen.2014.10.017.

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Bacterial nutrient foraging in a mouse model of enteral nutrient deprivation: Insight into the gut origin of sepsis
Ralls MW, Demehri FR, Feng Y, Raskind S, Ruan C, Schintlmeister A, Loy A, Hanson B, Berry D, Burant CF, Teitelbaum DH
Am J Physiol Gastrointest Liver Physiol. 2016 Sep 1:ajpgi.00088.2016. doi: 10.1152/ajpgi.00088.2016.

Small Mouse Islets Are Deficient in Glucagon-Producing Alpha Cells but Rich in Somatostatin-Secreting Delta Cells
Lau J, Grapengiesser, and Hellman B
Journal of Diabetes Research; Volume 2016 (2016), Article ID 4930741, 6 pages

Gene Expression Profile of Extracellular Matrix and Adhesion Molecules in the Human Normal Corneal Stroma
Liu Y, Huang H, Sun G, Alwadani S, Semba RD, Lutty GA, Yiu S, Edward DP
Curr Eye Res. 2016 Jul 21:1-8.

Live Cell Culture

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

Altered Transport Velocity of Axonal Mitochondria in Retinal Ganglion Cells After Laser-Induced Axonal Injury In Vitro
Yokota S, Takihara Y, Arimura S, Miyake S, Takamura Y, Yoshimura N, Inatani M
Invest Ophthalmol Vis Sci. 2015 Dec 1;56(13):8019-8025. doi: 10.1167/iovs.15-17876.

Isolation of single Chlamydia-infected cells using laser microdissection
Podgorny OV, Polina NF, Babenko VV, Karpova IY, Kostryukova ES, Govorun VM, Lazarev VN
J Microbiol Methods. 2014 Dec 26. pii: S0167-7012(14)00367-4. doi: 10.1016/j.mimet.2014.12.018.

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