Laser Microdissection Publication List

February 13, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection
Ma L, Li W, Song Q
Methods Mol Biol. 2017;1551:161-169. doi: 10.1007/978-1-4939-6750-6_9.

Adenocarcinoma arising in multiple hyperplastic polyps in a patient with Helicobacter pylori infection and hypergastrinemia during long-term proton pump inhibitor therapy
Anjiki H, Mukaisho KI, Kadomoto Y, Doi H, Yoshikawa K, Nakayama T, Vo DT, Hattori T, Sugihara H
Clin J Gastroenterol. 2017 Feb 3. doi: 10.1007/s12328-017-0714-7.

TP53 mutation-mediated genomic instability induces the evolution of chemoresistance and recurrence in epithelial ovarian cancer
Zhang M, Zhuang G, Sun X, Shen Y, Wang W, Li Q, Di W
Diagn Pathol. 2017 Feb 2;12(1):16. doi: 10.1186/s13000-017-0605-8.

RNA Research

Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
Pavličev M, Wagner GP, Chavan AR, Owens K, Maziarz J, Dunn-Fletcher C, Kallapur SG, Muglia L, Jones H
Genome Res. 2017 Feb 7. doi: 10.1101/gr.207597.116.

Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis
Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z
Journal of Genetics and Genomics, (2017), doi: 10.1016/j.jgg.2016.12.007

Alterations of p11 in brain tissue and peripheral blood leukocytes in Parkinson's disease
Green H, Zhang X, Tiklova K, Volakakis N, Brodin L, Berg L, Greengard P, Perlmann T, Svenningsson P
Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201621218. doi: 10.1073/pnas.1621218114.

Proteomics

Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology
Li G, Li M, Liang X, Xiao, Z, Zhang P, Shao M, Peng F, Chen Y, Li Y, Chen Z
Journal of Cancer, 2017; 8(3):  479-489. doi: 10.7150/jca.17192

Comparative proteomic analysis of urine and laser microdissected glomeruli in IgA nephropathy
Ning X, Yin Z, Li Z, Xu J, Wang L, Shen W, Lu Y, Cai G, Zhang X, Chen X
Clin Exp Pharmacol Physiol. 2017 Jan 21. doi: 10.1111/1440-1681.12733.

Synaptic Plasticity onto Dopamine Neurons Shapes Fear Learning
Pignatelli M, Umanah GK, Ribeiro SP, Chen R, Karuppagounder SS, Yau HJ, Eacker S, Dawson VL, Dawson TM, Bonci A
Neuron. 2017 Jan 18;93(2):425-440. doi: 10.1016/j.neuron.2016.12.030

Fluorescence Applications

Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'
Nakashima Y, Egami Y, Kimura M, Wakimoto T, Abe I
PLoS One. 2016 Oct 12;11(10):e0164468. doi: 10.1371/journal.pone.0164468. eCollection 2016.

Tracking the route of phenanthrene uptake in mosses: An experimental trial
Spagnuolo V, Figlioli F, De Nicola F, Capozzi F, Giordano S
Sci Total Environ. 2016 Sep 29. pii: S0048-9697(16)32104-0. doi: 10.1016/j.scitotenv.2016.09.174.

Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS
Xie W, Zhang H, Zeng J, Chen H, Zhao Z, Liang Z
Chem Cent J. 2016 Jul 13;10:42. doi: 10.1186/s13065-016-0187-7. eCollection 2016.

Cancer Research

Physical and Functional Interactions between ELL2 and RB in the Suppression of Prostate Cancer Cell Proliferation, Migration, and Invasion
Qiu X, Pascal LE, Song Q, Zang Y, Ai J, O’Malley KJ, Nelson JB, Zhou W
Neoplasia, Mar 2017, Volume 19, Issue 3, Pages 207–215

MicroRNA-155 Controls Exosome Synthesis and Promotes Gemcitabine Resistance in Pancreatic Ductal Adenocarcinoma
Mikamori M, Yamada D, Eguchi H, Hasegawa S, Kishimoto T, Tomimaru Y, Asaoka T, Noda T, Wada H, Kawamoto K, Gotoh K, Takeda Y, Tanemura M, Mori M, Doki Y
Sci Rep. 2017 Feb 15;7:42339. doi: 10.1038/srep42339.

Adenocarcinoma arising in multiple hyperplastic polyps in a patient with Helicobacter pylori infection and hypergastrinemia during long-term proton pump inhibitor therapy
Anjiki H, Mukaisho KI, Kadomoto Y, Doi H, Yoshikawa K, Nakayama T, Vo DT, Hattori T, Sugihara H
Clin J Gastroenterol. 2017 Feb 3. doi: 10.1007/s12328-017-0714-7.

Neurosciences

Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells
Cifuentes M, Baeza V, Arrabal PM, Visser R, Grondona JM, Saldivia N, Martínez F, Nualart F, Salazar K
Mol Neurobiol. 2017 Feb 13. doi: 10.1007/s12035-017-0434-5.

Alterations of p11 in brain tissue and peripheral blood leukocytes in Parkinson's disease
Green H, Zhang X, Tiklova K, Volakakis N, Brodin L, Berg L, Greengard P, Perlmann T, Svenningsson P
Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201621218. doi: 10.1073/pnas.1621218114.

MicroRNAs, miR-23a-3p and miR-151-3p, Are Regulated in Dentate Gyrus Neuropil following Induction of Long-Term Potentiation In Vivo
Ryan B, Logan BJ, Abraham WC, Williams JM
PLoS One. 2017 Jan 26;12(1):e0170407. doi: 10.1371/journal.pone.0170407.

Clinical Applications

Attenuation of alpha-naphthylisothiocyanate (ANIT)-induced biliary fibrosis by depletion of hepatic macrophages in rats
Golbar HM, Izawa T, Bondoc A, Wijesundera KK, Tennakoon AH, Kuwamura M, Yamate J
Exp Toxicol Pathol. 2017 Jan 31. pii: S0940-2993(16)30212-3. doi: 10.1016/j.etp.2017.01.005.

Targeting osteoblastic casein kinase-2 interacting protein-1 to enhance Smad-dependent BMP signaling and reverse bone formation reduction in glucocorticoid-induced osteoporosis
Liu J, Lu C, Wu X, Zhang Z, Li J, Guo B, Li D, Liang C, Dang L, Pan X, Peng S, Lu A, Zhang B, Zhang G
Sci Rep. 2017 Jan 27;7:41295. doi: 10.1038/srep41295.

Comparative proteomic analysis of urine and laser microdissected glomeruli in IgA nephropathy
Ning X, Yin Z, Li Z, Xu J, Wang L, Shen W, Lu Y, Cai G, Zhang X, Chen X
Clin Exp Pharmacol Physiol. 2017 Jan 21. doi: 10.1111/1440-1681.12733.

Developmental Research, Epigenetics & Aging

Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis
Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z
Journal of Genetics and Genomics, (2017), doi: 10.1016/j.jgg.2016.12.007

Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
Pavličev M, Wagner GP, Chavan AR, Owens K, Maziarz J, Dunn-Fletcher C, Kallapur SG, Muglia L, Jones H
Genome Res. 2017 Feb 7. doi: 10.1101/gr.207597.116.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Plant Research

Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells
Cifuentes M, Baeza V, Arrabal PM, Visser R, Grondona JM, Saldivia N, Martínez F, Nualart F, Salazar K
Mol Neurobiol. 2017 Feb 13. doi: 10.1007/s12035-017-0434-5.

Cell Wall Remodeling In Abscission Zone Cells During Ethylene-Promoted Fruit Abscission In Citrus
Merelo P, Agusti J, Arbona V, Costa ML, Estornell LH, Gomez-Cadenas A, Coimbra SV, Gomez MD, Perez-Amador MA, Domingo C, Talon M, and Tadeo FR
Front. Plant Sci. | doi: 10.3389/fpls.2017.00126

Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling
Martin LB, Nicolas P, Matas AJ, Shinozaki Y, Catalá C, Rose JK
Nat Protoc. 2016 Dec;11(12):2376-2388. doi: 10.1038/nprot.2016.146.

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Surfactant protein D regulates murine testicular immune milieu and sperm functions
Rokade S, Kishore U, Madan T
Am J Reprod Immunol. 2017 Jan 5. doi: 10.1111/aji.12629.

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp
Phan JL, Tucker MR, Khor SF, Shirley N, Lahnstein J, Beahan C, Bacic A, Burton RA
J Exp Bot. 2016 Dec;67(22):6481-6495.

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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