Laser Microdissection Publication List

June 08, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Variability of DNA Methylation within Schizophrenia Risk Loci across Subregions of Human Hippocampus
Ruzicka WB, Subburaju S, Benes FM
Genes (Basel). 2017 May 15;8(5). pii: E143. doi: 10.3390/genes8050143.

Cancer-Associated Mutations in Endometriosis without Cancer
Anglesio MS, Papadopoulos N, Ayhan A, Nazeran TM, Noë M, Horlings HM, Lum A, Jones S, Senz J, Seckin T, Ho J, Wu RC, Lac V, Ogawa H, Tessier-Cloutier B, Alhassan R, Wang A, Wang Y, Cohen JD, Wong F, Hasanovic A, Orr N, Zhang M, Popoli M, McMahon W, Wood LD, Mattox A, Allaire C, Segars J, Williams C, Tomasetti C, Boyd N, Kinzler KW, Gilks CB, Diaz L, Wang TL, Vogelstein B, Yong PJ, Huntsman DG, Shih IM
N Engl J Med. 2017 May 11;376(19):1835-1848. doi: 10.1056/NEJMoa1614814.

Purification of leukemic blast cells from blood smears using laser microdissection
Matsuo H, Shiga S, Imai T, Kamikubo Y, Toki T, Terui K, Ito E, Adachi S
Int J Hematol. 2017 Apr 13. doi: 10.1007/s12185-017-2227-z.

RNA Research

The let-7/lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury
McDaniel K, Huang L, Sato K, Wu N, Annable T, Zhou T, Ramos-Lorenzo S, Wan Y, Huang Q, Francis H, Glaser S, Tsukamoto H, Alpini G, Meng F
J Biol Chem. 2017 May 23. pii: jbc.M116.773291. doi: 10.1074/jbc.M116.773291.

TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Kim JB, Pjanic M, Nguyen T, Miller CL, Iyer D, Liu B, Wang T, Sazonova O, Carcamo-Orive I, Matic LP, Maegdefessel L, Hedin U, Quertermous T
PLoS Genet. 2017 May 8;13(5):e1006750. doi: 10.1371/journal.pgen.1006750.

Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics
Verckist L, Lembrechts R, Thys S, Pintelon I, Timmermans JP, Brouns I, Adriaensen D
Respir Res. 2017 May 8;18(1):87. doi: 10.1186/s12931-017-0571-4.

Proteomics

Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC-MS/MS
Xiao CJ, Liu YC, Luo SH, Hua J, Liu Y, Li SH
Phytochem Anal. 2017 May 9. doi: 10.1002/pca.2687.

Sushi repeat-containing protein 1: a novel disease-associated molecule in cerebral amyloid angiopathy
Inoue Y, Ueda M, Tasaki M, Takeshima A, Nagatoshi A, Masuda T, Misumi Y, Kosaka T, Nomura T, Mizukami M, Matsumoto S, Yamashita T, Takahashi H, Kakita A, Ando Y
Acta Neuropathol. 2017 May 6. doi: 10.1007/s00401-017-1720-z.

A selected reaction monitoring mass spectrometric assessment of biomarker candidates diagnosing large-cell neuroendocrine lung carcinoma by the scaling method using endogenous references
Fukuda T, Nomura M, Kato Y, Tojo H, Fujii K, Nagao T, Bando Y, Fehniger TE, Marko-Varga G, Nakamura H, Kato H, Nishimura T
PLoS One. 2017 Apr 27;12(4):e0176219. doi: 10.1371/journal.pone.0176219. eCollection 2017.

Fluorescence Applications

The let-7/lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury
McDaniel K, Huang L, Sato K, Wu N, Annable T, Zhou T, Ramos-Lorenzo S, Wan Y, Huang Q, Francis H, Glaser S, Tsukamoto H, Alpini G, Meng F
J Biol Chem. 2017 May 23. pii: jbc.M116.773291. doi: 10.1074/jbc.M116.773291.

Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics
Verckist L, Lembrechts R, Thys S, Pintelon I, Timmermans JP, Brouns I, Adriaensen D
Respir Res. 2017 May 8;18(1):87. doi: 10.1186/s12931-017-0571-4.

Local Inflammatory Cues Regulate Differentiation and Persistence of CD8+ Tissue-Resident Memory T Cells
Bergsbaken T1, Bevan MJ2, Fink PJ
Cell Rep. 2017 Apr 4;19(1):114-124. doi: 10.1016/j.celrep.2017.03.031.

Cancer Research

A selected reaction monitoring mass spectrometric assessment of biomarker candidates diagnosing large-cell neuroendocrine lung carcinoma by the scaling method using endogenous references
Fukuda T, Nomura M, Kato Y, Tojo H, Fujii K, Nagao T, Bando Y, Fehniger TE, Marko-Varga G, Nakamura H, Kato H, Nishimura T
PLoS One. 2017 Apr 27;12(4):e0176219. doi: 10.1371/journal.pone.0176219. eCollection 2017.

Cancer-Associated Mutations in Endometriosis without Cancer
Anglesio MS, Papadopoulos N, Ayhan A, Nazeran TM, Noë M, Horlings HM, Lum A, Jones S, Senz J, Seckin T, Ho J, Wu RC, Lac V, Ogawa H, Tessier-Cloutier B, Alhassan R, Wang A, Wang Y, Cohen JD, Wong F, Hasanovic A, Orr N, Zhang M, Popoli M, McMahon W, Wood LD, Mattox A, Allaire C, Segars J, Williams C, Tomasetti C, Boyd N, Kinzler KW, Gilks CB, Diaz L, Wang TL, Vogelstein B, Yong PJ, Huntsman DG, Shih IM
N Engl J Med. 2017 May 11;376(19):1835-1848. doi: 10.1056/NEJMoa1614814.

Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture microdissection and cycling temperature capillary electrophoresis
Refinetti P, Arstad C, Thilly WG, Morgenthaler S, Ekstrøm PO
BMC Clinical Pathology (2017) 17:6 DOI 10.1186/s12907-017-0042-3

Neurosciences

Variability of DNA Methylation within Schizophrenia Risk Loci across Subregions of Human Hippocampus
Ruzicka WB, Subburaju S, Benes FM
Genes (Basel). 2017 May 15;8(5). pii: E143. doi: 10.3390/genes8050143.

Serum amyloid P component: A novel potential player in vessel degeneration in CADASIL
Nagatoshi A, Ueda M, Ueda A, Tasaki M, Inoue Y, Ma Y, Masuda T, Mizukami M, Matsumoto S, Kosaka T, Kawano T, Ito T, Ando Y
Jns(2017), doi: 10.1016/j.jns.2017.05.033

GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients
Ban N, Siegfried CJ, Lin JB, Shui YB, Sein J, Pita-Thomas W, Sene A, Santeford A, Gordon M, Lamb R, Dong Z, Kelly SC, Cavalli V, Yoshino J, Apte RS
JCI Insight. 2017 May 4;2(9). pii: 91455. doi: 10.1172/jci.insight.91455.

Clinical Applications

The let-7/lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury
McDaniel K, Huang L, Sato K, Wu N, Annable T, Zhou T, Ramos-Lorenzo S, Wan Y, Huang Q, Francis H, Glaser S, Tsukamoto H, Alpini G, Meng F
J Biol Chem. 2017 May 23. pii: jbc.M116.773291. doi: 10.1074/jbc.M116.773291.

TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Kim JB, Pjanic M, Nguyen T, Miller CL, Iyer D, Liu B, Wang T, Sazonova O, Carcamo-Orive I, Matic LP, Maegdefessel L, Hedin U, Quertermous T
PLoS Genet. 2017 May 8;13(5):e1006750. doi: 10.1371/journal.pgen.1006750.

Differential involvement during latent HSV-1 infection of the superior and inferior divisions of the vestibular ganglia: implications for vestibular neuritis
Himmelein S, Lindemann A, Sinicina I, Horn AKE, Brandt T, Strupp M, Hüfner K
J Virol. 2017 Apr 26. pii: JVI.00331-17. doi: 10.1128/JVI.00331-17.

Developmental Research, Epigenetics & Aging

The effects of IGF-1 on mouse spermatogenesis using an organ culture method
Yao J, Zuo H, Gao J, Wang M, Wang D, Li X
Biochem Biophys Res Commun. 2017 May 25. pii: S0006-291X(17)31018-5.

Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics
Verckist L, Lembrechts R, Thys S, Pintelon I, Timmermans JP, Brouns I, Adriaensen D
Respir Res. 2017 May 8;18(1):87. doi: 10.1186/s12931-017-0571-4.

Expression and trafficking of placental microRNAs at the feto-maternal interface
Chang G, Mouillet JF, Mishima T, Chu T, Sadovsky E, Coyne CB, Parks WT, Surti U, Sadovsky Y
FASEB J. 2017 Mar 13. pii: fj.201601146R. doi: 10.1096/fj.201601146R.

Plant Research

Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC-MS/MS
Xiao CJ, Liu YC, Luo SH, Hua J, Liu Y, Li SH
Phytochem Anal. 2017 May 9. doi: 10.1002/pca.2687.

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Localization of a Defensive Volatile 4-Hydroxy-4-methylpentan-2-one in the Capitate Glandular Trichomes of Oenothera glazioviana
Tan YY, Li DS, Hua J, Luo SH, Liu Y, Li SH
Plant Diversity, 17 May 2017, ISSN 2468-2659, doi.org/10.1016/j.pld.2017.05.004.

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Motility in liquid and semisolid media of Paenibacillus polymyxa associative rhizobacteria differing in exopolysaccharide yield and properties
Yegorenkova IV, Tregubova KV, Schelud’ko AV
Symbiosis (2017). doi:10.1007/s13199-017-0492-5

Microbial formation of labile organic carbon in Antarctic glacial environments
Smith HJ, Foster RA, McKnight DM, Lisle JT, Littmann S Kuypers MMM, Foreman CM
Nature Geoscience, 3 Apr 2017 | DOI: 10.1038/NGEO2925

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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