Laser Microdissection Publication List

August 23, 2017

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

DNA Research

Comparison of ESR1 Mutations in Tumor Tissue and Matched Plasma Samples from Metastatic Breast Cancer Patients
Takeshita T, Yamamoto Y, Yamamoto-Ibusuki M, Tomiguchi M, Sueta A, Murakami K, Omoto Y, Iwase H
Transl Oncol. 2017 Jul 31;10(5):766-771. doi: 10.1016/j.tranon.2017.07.004.

Concordance between comprehensive cancer genome profiling in plasma and tumor specimens
Müller JN, Falk M, Talwar J, Neemann N, Mariotti E, Bertrand M, Zacherle T, Lakis S, Menon R, Gloeckner C, Tiemann M, Heukamp LC, Thomas RK, Griesinger F, Heuckmann JM
J Thorac Oncol. 2017 Jul 24. pii: S1556-0864(17)30611-1. doi: 10.1016/j.jtho.2017.07.014.

Heterogeneous DNA methylation status in same-cell subpopulations of ovarian cancer tissues
Li Q, Xue X, Li W, Wang Q, Han L, Brunson T, Xu W, Chambers-Harris I, Wang Q, Li X, Ma L, Song Q
Tumour Biol. 2017 Jun;39(6):1010428317701650. doi: 10.1177/1010428317701650.

RNA Research

ANK1 is up-regulated in laser captured microglia in Alzheimer's brain; the importance of addressing cellular heterogeneity
Mastroeni D, Sekar S, Nolz J, Delvaux E, Lunnon K, Mill J, Liang WS, Coleman PD
PLoS One. 2017 Jul 12;12(7):e0177814. doi: 10.1371/journal.pone.0177814. eCollection 2017.

Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure
De Santis S, Galleggiante V, Scandiffio L, Liso M, Sommella E, Sobolewski A, Spilotro V, Pinto A, Campiglia P, Serino G, Santino A, Notarnicola M, Chieppa M
Nutrients. 2017 Jul 6;9(7). pii: E706. doi: 10.3390/nu9070706.

Intestinal cancer progression by mutant p53 through the acquisition of invasiveness associated with complex glandular formation
Nakayama M, Sakai E, Echizen K, Yamada Y, Oshima H, Han TS, Ohki R, Fujii S, Ochiai A, Robine S, Voon DC, Tanaka T, Taketo MM, Oshima M
Oncogene. 2017 Jun 19. doi: 10.1038/onc.2017.194.

Proteomics

Vitreous amyloidosis with autonomic neuropathy of the digestive tract associated with a novel transthyretin p.Gly87Arg variant in a Bangladeshi patient: a case report
Terrier B, Colombat M, Beugnet C, Quéant A, London J, Daudin JB, Le Jeunne C, Mouthon L, Monnet D, Cauquil C, Lacroix C, Adams D, Brézin A, Valleix S
J Med Case Rep. 2017 Aug 13;11(1):222. doi: 10.1186/s13256-017-1407-z.

DNAJB9 is a Specific Immunohistochemical Marker for Fibrillary Glomerulonephritis
Nasr SH, Vrana JA, Dasari S, Bridoux F, Fidler ME, Kaaki S, Quellard N, Rinsant A, Goujon JM, Sethi S, Fervenza FC, Cornell LD, Said SM, McPhail E, Herrera Hernandez LP, Grande JP, Hogan MC, Lieske JC, Leung N, Kurtin PJ, Alexander MP
Kidney International Reports (31 Jul 2017), doi: 10.1016/j.ekir.2017.07.017.

PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples
Noberini R, Longuespée R, Richichi C, Pruneri G, Kriegsmann M, Pelicci G, Bonaldi T
Clin Epigenetics. 2017 Jul 11;9:69. doi: 10.1186/s13148-017-0369-8. eCollection 2017.

Fluorescence Applications

Endothelial Cell Heterogeneity: A Correlation With The Von Willebrand Factor Expression In Distinct Organs
Carrillo MAL
Thesis, 2017

Participation of Tumor-Associated Myeloid Cells in Progression of Amelanotic Melanoma (RMM Tumor Line) in F344 Rats, with Particular Reference to MHC Class II- and CD163-Expressing Cells
Bondoc A, Golbar HM, Pervin M, Katou-Ichikawa C, Tanaka M, Izawa T, Kuwamura M, Yamate J
Cancer Microenviron. 2017 Jun 16. doi: 10.1007/s12307-017-0193-x.

Tissue based metabolite profiling and qualitative comparison of two species of Achyranthes roots by use of UHPLC-QTOF MS and laser micro dissection
Jaiswal Y, Liang Z, Ho A, Chen H, Williams L, Zhao Z
Journal of Pharmaceutical Analysis, 16 June 2017

Cancer Research

Comparison of ESR1 Mutations in Tumor Tissue and Matched Plasma Samples from Metastatic Breast Cancer Patients
Takeshita T, Yamamoto Y, Yamamoto-Ibusuki M, Tomiguchi M, Sueta A, Murakami K, Omoto Y, Iwase H
Transl Oncol. 2017 Jul 31;10(5):766-771. doi: 10.1016/j.tranon.2017.07.004.

Concordance between comprehensive cancer genome profiling in plasma and tumor specimens
Müller JN, Falk M, Talwar J, Neemann N, Mariotti E, Bertrand M, Zacherle T, Lakis S, Menon R, Gloeckner C, Tiemann M, Heukamp LC, Thomas RK, Griesinger F, Heuckmann JM
J Thorac Oncol. 2017 Jul 24. pii: S1556-0864(17)30611-1. doi: 10.1016/j.jtho.2017.07.014.

PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples
Noberini R, Longuespée R, Richichi C, Pruneri G, Kriegsmann M, Pelicci G, Bonaldi T
Clin Epigenetics. 2017 Jul 11;9:69. doi: 10.1186/s13148-017-0369-8. eCollection 2017.

Neurosciences

ANK1 is up-regulated in laser captured microglia in Alzheimer's brain; the importance of addressing cellular heterogeneity
Mastroeni D, Sekar S, Nolz J, Delvaux E, Lunnon K, Mill J, Liang WS, Coleman PD
PLoS One. 2017 Jul 12;12(7):e0177814. doi: 10.1371/journal.pone.0177814. eCollection 2017.

Lower affinity of isradipine for L-type Ca2+ channels during substantia nigra dopamine neuron-like activity: implications for neuroprotection in Parkinson's disease
Ortner NJ, Bock G, Dougalis A, Kharitonova M, Duda J, Hess S, Tuluc P, Pomberger T, Stefanova N, Pitterl F, Ciossek T, Oberacher H, Draheim HJ, Kloppenburg P, Liss B, Striessnig J
J Neurosci. 2017 Jun 7. pii: 2946-16. doi: 10.1523/JNEUROSCI.2946-16.2017.

Development of transcripts regulating dendritic spines in layer 3 pyramidal cells of the monkey prefrontal cortex: Implications for the pathogenesis of schizophrenia
Dienel SJ, Bazmi HH, Lewis DA
Neurobiol Dis. 2017 May 30. pii: S0969-9961(17)30125-0. doi: 10.1016/j.nbd.2017.05.016.

Clinical Applications

Vitreous amyloidosis with autonomic neuropathy of the digestive tract associated with a novel transthyretin p.Gly87Arg variant in a Bangladeshi patient: a case report
Terrier B, Colombat M, Beugnet C, Quéant A, London J, Daudin JB, Le Jeunne C, Mouthon L, Monnet D, Cauquil C, Lacroix C, Adams D, Brézin A, Valleix S
J Med Case Rep. 2017 Aug 13;11(1):222. doi: 10.1186/s13256-017-1407-z.

DNAJB9 is a Specific Immunohistochemical Marker for Fibrillary Glomerulonephritis
Nasr SH, Vrana JA, Dasari S, Bridoux F, Fidler ME, Kaaki S, Quellard N, Rinsant A, Goujon JM, Sethi S, Fervenza FC, Cornell LD, Said SM, McPhail E, Herrera Hernandez LP, Grande JP, Hogan MC, Lieske JC, Leung N, Kurtin PJ, Alexander MP
Kidney International Reports (31 Jul 2017), doi: 10.1016/j.ekir.2017.07.017.

Ethambutol partitioning in tuberculous pulmonary lesions explains its clinical efficacy
Zimmerman M, Lestner J, Prideaux B, O'Brien P, Freedman I, Chen C, Dietzold J, Daudelin I, Kaya F, Blanc L, Chen PY, Park S, Salgame P, Sarathy J, Dartois V
Antimicrob Agents Chemother. 2017 Jul 10. pii: AAC.00924-17. doi: 10.1128/AAC.00924-17.

Developmental Research, Epigenetics & Aging

Expression of the matrix gla protein (MGP) gene in different developmental stages of mouse mandibular first molar root
Wang R, Dang, P, Niu Z
Biomedical Research, Jun 2017

The effects of IGF-1 on mouse spermatogenesis using an organ culture method
Yao J, Zuo H, Gao J, Wang M, Wang D, Li X
Biochem Biophys Res Commun. 2017 May 25. pii: S0006-291X(17)31018-5.

Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics
Verckist L, Lembrechts R, Thys S, Pintelon I, Timmermans JP, Brouns I, Adriaensen D
Respir Res. 2017 May 8;18(1):87. doi: 10.1186/s12931-017-0571-4.

Plant Research

Tissue based metabolite profiling and qualitative comparison of two species of Achyranthes roots by use of UHPLC-QTOF MS and laser micro dissection
Jaiswal Y, Liang Z, Ho A, Chen H, Williams L, Zhao Z
Journal of Pharmaceutical Analysis, 16 June 2017

Localisation of Two Bioactive Labdane Diterpenoids in the Peltate Glandular Trichomes of Leonurus japonicus by Laser Microdissection Coupled with UPLC-MS/MS
Xiao CJ, Liu YC, Luo SH, Hua J, Liu Y, Li SH
Phytochem Anal. 2017 May 9. doi: 10.1002/pca.2687.

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Forensics

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Mitochondrial DNA Typing of Laser-captured Single Sperm Cells to Differentiate Individuals in a Mixed Semen Stain
Zhang L, Ding M, Pang H, Xing J, Xuan J, Wang C, Lin Z, Han S, Liang K, Li C, Yao J, Wang B
Electrophoresis. 2016 May 26. doi: 10.1002/elps.201600009.

Optimization of Spermatozoa Detection using Immunofluorescent Staining and Laser Micro-Dissection
Ping YS, Shun Chang XL, Goh SK, Choong Syn CK
Forensic Science International, 13 Jul 2015

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification
Kanwar N, Done SJ
Methods Mol Biol. 2017;1634:143-152. doi: 10.1007/978-1-4939-7144-2_11.

Crenothrix are major methane consumers in stratified lakes
Oswald K, Graf JS, Littmann S, Tienken D, Brand A, Wehrli B, Albertsen M, Daims H, Wagner M, Kuypers MM, Schubert CJ, Milucka J
ISME J. 2017 Jun 6. doi: 10.1038/ismej.2017.77.

The impact of drought on wheat leaf cuticle properties
Bi H, Kovalchuk N, Langridge P, Tricker PJ, Lopato S, Borisjuk N
BMC Plant Biol. 2017 May 8;17(1):85. doi: 10.1186/s12870-017-1033-3

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.

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