Micro-Computed Tomography (micro-CT) of Book Scorpion Musculature

Application Note for Leica EM CPD300 - Life Science Research

September 01, 2016

Introduction
Species: Book scorpion (Neobisium sp.)
Critical point drying of book scorpion with subsequent X-ray micro-computed tomography (micro-CT) to detect anatomical features with special regard to the musculature.

Procedure
Sample Holder: Sample was transferred to microporous specimen pot and placed into chamber of Filter Discs and Porous Pots holder.


Fixation and Dehydration

Ethanol (70%)overnight
Ethanol series: 80%, 90%, 96%, 100%2x10 min.
Iodine staining (1% iodine solution in 100% ethanol)overnight
Ethanol: 100%2x10 min.

Leica EM CPD300 auto Program:

Fig.1: Volume reconstruction of a book scorpion (outer view and inner view to visualize the musculature).

Comments

Help us to improve the Leica Science Lab and participate in our survey! Start Survey

Arrow Down

Arrow Up