The filter cube determines the imaging results
Fluorescence microscopy harnesses the ability of fluorescent dyes or fluorescent proteins to emit light after excitation with light of a certain wavelength. Using optical filters, which are often plugged together in a filter cube, the excitation light is blocked and the labeled sample can be imaged on a dark background with high sensitivity.
To achieve optimal results in fluorescence microscopy, the light source, the fluorophores and the filter cubes have to match perfectly. A fluorescence microscope with a perfect fit of excitation and emission filter set is able to maximize the efficiency of excitation and emission of the fluorophores and thus provide crisp fluorescence images.
Leica Microsystems FluoScout™
FluoScout™ is an easy-to-use and reliable tool for getting the most out of fluorescence microscopy. Based on the user's choice of light sources and fluorophores it provides a recommendation of filter cubes. The spectrum of visible light is shown as a constant reference. To obtain a better overview the user can fade in and out single spectra, or zoom into them to see important details. Transmission and emission can also be quantified using a slider.
Various smart functions make the FluoScout™ especially user-friendly. To get started a help function and a tooltip guide users through the site. The selected spectra can be shared with colleagues by e-mail or bookmarked using the link creator. A contact button is provided for posting questions directly to Leica Microsystems’ support team. Just one click on the quotation is enough to request offers.
The TripleBeam™ principle of stereo microscopes
Leica Microsystems’ Fluorescence Stereo Microscopes use TripleBeam™ technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror. Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.