Online Tool to Determine the Optimal Combination of Fluorescence Filter Cubes, Fluorophores and Light Sources

Leica Microsystems FluoScout™ Helps Researchers to Get the Most out of Their Fluorescence Microscope

August 19, 2013

To achieve optimal results in fluorescence microscopy, the light source, the fluorophores and the filter cubes have match perfectly. A fluorescence microscope with a perfect fit of excitation and emission filter set is able to maximize the efficiency of excitation and emission of the fluorophores and thus provide crisp fluorescence images.

The online tool Leica Microsystems FluoScout™ helps users achieve excellent fluorescence imaging results by recommending the optimum filter cube and filter cube set for their choice of fluorophores.

The filter cube determines the imaging results

Fluorescence microscopy harnesses the ability of fluorescent dyes or fluorescent proteins to emit light after excitation with light of a certain wavelength. Using optical filters, which are often plugged together in a filter cube, the excitation light is blocked and the labeled sample can be imaged on a dark background with high sensitivity.

To achieve optimal results in fluorescence microscopy, the light source, the fluorophores and the filter cubes have to match perfectly. A fluorescence microscope with a perfect fit of excitation and emission filter set is able to maximize the efficiency of excitation and emission of the fluorophores and thus provide crisp fluorescence images.

Leica Microsystems FluoScout™

FluoScout™ is an easy-to-use and reliable tool for getting the most out of fluorescence microscopy. Based on the user's choice of light sources and fluorophores it provides a recommendation of filter cubes. The spectrum of visible light is shown as a constant reference. To obtain a better overview the user can fade in and out single spectra, or zoom into them to see important details. Transmission and emission can also be quantified using a slider.

Various smart functions make the FluoScout™ especially user-friendly. To get started a help function and a tooltip guide users through the site. The selected spectra can be shared with colleagues by e-mail or bookmarked using the link creator. A contact button is provided for posting questions directly to Leica Microsystems’ support team. Just one click on the quotation is enough to request offers.

Start Leica Microsystems FluoScout

The TripleBeam™ principle of stereo microscopes

Leica Microsystems’ Fluorescence Stereo Microscopes use TripleBeam™ technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror. Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.

Fig. 1: The online tool FluoScout™ enables users of fluorescence microscopes to determine the best filter cube or filter set for achieving excellent imaging results.
Fig. 2: Selecting the light source. The right light source can be selected from a list of different available light sources for Leica fluorescence microscopes. The spectrum of the selected light source will be displayed.
Fig. 3: Adding fluorophores. Select the fluorophore you want. After clicking on a fluorophore in the displayed list, you will get a preview of the excitation and emission spectrum preview. When you click "ok", the fluorophore will be added.
Fig. 4: Recommended filter cubes / filter sets. Depending on the selected fluorophore(s), list of fluorescence filter cubes is shown, whereas recommended filter cubes are marked with an asterisk.
Fig. 5: Adding more filter cubes. Once you have added one filter cube, more can be added just by opening the filter cube list and selecting them.

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