Protein Trafficking in Cereal Seeds

February 10, 2014

Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to 80% of the total seed volume [1]. The seed endomembrane system is highly specialized and seed storage proteins travel through the endomembrane system en route to the protein bodies, which are either derived from the Endoplasmic reticulum (ER) or of vacuolar origin [2]. In most cereals prolamins are the main class of storage proteins which form ER derived protein bodies. However, some species like wheat, oat or barley contain variable amounts of both prolamins and globulins which accumulate in protein storage vacuoles.

Gaining deeper insights with fluorescence and electron microscopy

The natural ability of cereal seeds to store proteins in a stable environment has successfully been exploited to use cereals as platforms for the production of recombinant proteins of pharmaceutical interest [3, 4, 5]. For this reason, the investigation of the different trafficking routes for proteins has been subject of several studies, since the final storage site as well as the route followed by the recombinant protein have an influence in the final yield and quality of such product.

The use of fluorescence and electron microscopy in combination with the analysis of glycan structures on recombinant glycoproteins produced in different systems (monocots and dicots) has allowed to gain a deeper insight into the topic. Indeed, we have proved the presence of protein storage vacuoles in maize [6] and we have demonstrated crucial differences in the trafficking of proteins in cereal endosperm and dicot seeds. Thus, in cereal endosperm cells the protein storage vacuole is the preferred destination for recombinant proteins that enter the endomembrane system and do not carry further targeting signals [7, 8]. The same proteins are efficiently secreted in dicot seeds like tobacco or Arabidopsis, reflecting the high specialization of the cereal endosperm, a moribund tissue which does not survive beyond germination and whose only purpose is the storage of proteins and starch [9].

Deposition of recombinant fungal phytase in maize endosperm cells

Fig. 1: Deposition of recombinant fungal phytase in maize endosperm cells

Figure 1 shows the deposition of recombinant fungal phytase in maize endosperm cells. Recombinant fungal phytase carrying a signal peptide and no further targeting signal was expressed in maize endosperm. Slices of endosperm corresponding to a mid developmental stage were cut with a razor blade and fixed in 4 % paraformaldehyde and 0,5 % glutaraldehyde.

After dehydration through ethanol series, samples were infiltrated and embedded in LRWhite resin and the blocks were allowed to polymerise at 60 °C. Semithin sections (1 µm) were obtained with a Leica Ultracut UCT and stained with toluidine blue (A) or used for immunofluorescence studies (B).

Ultrathin sections (80 nm) were also obtained and stained with uranyl acetate 1 % and lead citrate 3 % in a Leica EM AC20 after immunogold localization of recombinant phytase. Light and immunofluorescence pictures were taken with a Leica DM5500. Ultrathin sections were observed with a Philips EM-400 electron microscope.

See the different protein storage organelles in a mid developmental stage endosperm cell: protein storage vacuoles (arrows) and zein bodies (arrowheads). Phytase is unexpectedly deposited in the protein storage vacuole (B, arrow; C, PSV, arrow) as well as around the zein bodies (C, zb). Starch (s). Bars 10 µm (A, B), 1 µm (C).

References

  1. Watson SA: Structure and composition. In: Watson SA and Ramstad PT (eds.): Corn: Chemistry and Technology. American Association of Cereal Chemists pp. 53–82 (1987).
  2. Muntz K: Deposition of storage proteins. Plant Mol. Biol. 38 (1–2): 77–99 (1998).
  3. Hood EE, Witcher DR, Maddock S, Meyer T, Baszczynski C, Bailey MR, Flynn P, Register J, Marschall L, Bond D, Kulisek E, Kusnadi A, Evangelista R, Nikolov Z, Wooge C, Mehigh RJ, Hernan R, Kappel WK, Ritland D, Li C-P and Howard JA: Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol. Breed 3: 291–306 (1997).
  4. Witcher DR, Hood EE, Peterson D, Bailey M, Bond D, Kusnadi A, Evangelista R, Nikolov Z, Wooge C, Mehigh R, Kappel W, Register I, James C and Howard JA: Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants. Mol. Breed 4: 301–12 ( (1998).
  5. Devaiah SP, Requesens DV, Chang YK, Hood KR, Flory A, Howard JA and Hood EE: Heterologous expression of cellobiohydrolase II (Cel6A) in maize endosperm. Transgenic Res. 22: 477–88 (2013).
  6. Arcalis E, Stadlmann J, Marcel S, Drakakaki G, Winter V, Rodriguez J, Fischer R, Altmann F, Stoger E: The changing fate of a secretory glycoprotein in developing maize endosperm. Plant Physiol. 153 (2): 693–702 (2010).
  7. Arcalis E, Marcel S, Altmann F, Kolarich D, Drakakaki G, Fischer R,Christou P and Stoeger E: Unexpected deposition patterns of recombinant proteins in post-endospermic reticulum compartments of wheat endosperm. Plant Physiol. 136: 3457–66 (2004).
  8. Drakakaki G, Marcel S, Arcalis E, Altmann F, Gonzalez-Melendi P, Fischer R, Christou P and Stoeger E: The intracellular fate of a recombinant protein in tissue dependent. Plant Physiol. 141: 578–86 (2006).
  9. Arcalis E, Stadlmann J, Rademacher T, Marcel S, Sack M, Altmann F and Stoeger E: Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins Plant Mol Biol. 83 (1–2): 105–17 (2013).

Comments