http://www.leica-microsystems.com/ Article tagged with en-US Telecentric lenses have existed for decades, but are not available on every microscope system. "Telecentricity" is an important design feature of an optical system, in which all rays passing through the system are very nearly collimated and parallel to the optical axis in the image space, the object space, or both. The ad http://www.leica-microsystems.com/science-lab/webinars/webinar-how-more-accurate-and-reproducible-measurements-can-be-made-through-telecentricity/ Tue, 18 Jun 2013 10:19:00 +0200 Clinton Smith, Mario J. Gislao Lasers have been critical to the advancement on confocal microscopy, and the white light laser (WLL) offers particular advantages. Finessing WLL output for bioimaging is a complex task, though, and traditional approaches retain key limitations. But acousto-optical beamsplitting enables smoother operation, leading to enhanced microscopy capabilities. http://www.leica-microsystems.com/science-lab/multiple-microscopy-modes-in-a-single-sweep-with-supercontinuum-white-light/ Fri, 14 Jun 2013 16:09:00 +0200 Dr. Rolf T. Borlinghaus, Dr. Lioba Kuschel Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant structural alterations is by cryo-fixation. There are currently two common methods employed; plunge freezing and high pressure freezing. http://www.leica-microsystems.com/science-lab/brief-introduction-in-high-pressure-freezing/ Wed, 12 Jun 2013 19:59:00 +0200 Dr. Cveta Tomova The discovery of green fluorescent protein a half century ago heralded a new and explosive era in microscopy, forever changing the landscape for biology imaging. The ability to fuse a genetically encoded fluorescent probe to an almost-unlimited variety of proteins has enabled scientists to investigate signaling pathways and the movement of intracellular proteins in living cells with unprecedented detail, particularly when coupled with powerful widefield fluorescence and confocal microscopy techniques. http://www.leica-microsystems.com/science-lab/webinars/webinar-fluorescent-probes-and-digital-imaging-where-we-are-and-where-were-going/ Wed, 12 Jun 2013 15:37:00 +0200 PhD Marcel Bruchez, PhD William Esco Moerner, Professor Alan S. Waggoner This step by step video tutorials show how to align the ion beams and how to replace the ion sources of the ion beam milling system Leica EM RES102. http://www.leica-microsystems.com/science-lab/video-tutorials-how-to-align-the-ion-beams-and-how-to-replace-the-ion-source-of-the-ion-beam-milling-system-leica-em-res102/ Fri, 07 Jun 2013 11:06:00 +0200 Thomas Pfeifer, Robert Ranner This webinar will illustrate results obtained by biochemical, Epifluorescence, TIRFM, Confocal and GSD techniques. Depending on the aim of experimental question, different imaging techniques deliver insights into varying aspects of intracellular pathways. To achieve "True-to-detail imaging" of the spatial arrangement of proteins and other biomolecules in cells, GSDIM achieves resolutions up to 20 nm in x and y direction – beyond the diffraction limit of light microscopy. But Super-resolution microscopy can be applied in the axial (z-) direction, too. A recent commercial implementation of the astigmatism approach will be discussed in more detail during this webinar. http://www.leica-microsystems.com/science-lab/webinars/webinar-from-epifluorescence-to-super-resolution-in-3d/ Tue, 04 Jun 2013 12:53:00 +0200 Dr. Nathalie Garin, Prof. Ralf Jacob Until now, physicians have largely been skeptical of the advantages of 3D technology. But this may be about to change: the findings of a new study show that even experienced surgeons stand to benefit from the third dimension. http://www.leica-microsystems.com/science-lab/microsurgery/new-opportunities-for-3d-technology-in-medicine/ Wed, 29 May 2013 16:55:00 +0200 Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing the pinhole depends on the area of that aperture, consequently the area is the parameter that must be compared when discussing brightness of focus images. The use of incommensurable edge lengths is meant to confuse the reader and thus dishonest and reprehensible. In this article, the signal level as a function of geometry and size in confocal microscopes is described. http://www.leica-microsystems.com/science-lab/pinhole-geometry-four-corners-are-perfect/ Thu, 23 May 2013 14:59:00 +0200 Dr. Rolf T. Borlinghaus A deeper understanding of brain function requires visualization of the complex architecture of neurons and their connections. In this special webinar, two researchers working at the cutting-edge of brain imaging will discuss their applications of the latest confocal and two-photon microscopy techniques. From fluorescent labeling and imaging of neural circuits using the Brainbow system to in vivo imaging of brain structure, function, and blood flow, this webinar will give researchers a deeper appreciation of the potential for new microscopy methods to unlock the secrets of the cellular world. Unique insights into sample handling and processing of multicolor images will also be presented, and attendees will have the opportunity to ask questions. http://www.leica-microsystems.com/science-lab/webinars/webinar-confocal-and-two-photon-microscopy-methods-for-imaging-the-brain/ Wed, 22 May 2013 14:20:00 +0200 PhD, Assistant Professor Ian R. Winship, PhD Dawen Cai Fluorescence recovery after photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. State of the art laser scanning microscopes such as the Leica TCS SP8 have the advantage of using a high intensity laser for bleaching and a low intensity laser for image recording. The LAS AF application wizard offers different ways to carry out a FRAP experiment. http://www.leica-microsystems.com/science-lab/step-by-step-guide-for-frap-experiments/ Fri, 17 May 2013 15:50:00 +0200 Dr. Jan Schröder