STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging

March 01, 2013

This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning microscopy – usually an extension of a confocal microscope. Ground state depletion imaging (GSDIM) is a parallel recording widefield approach that explores inherent switching of fluorochromes and typically comes with a TIRF microscope. The two methods use very different approaches to reach the same goal: to see more details in light microscopes than possible when diffraction limited.

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Borlinghaus RT:
STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging

Méndez-Vilas A (ed.): Current microscopy contributions to advances in science and technology. Formatex Vol. 2, ISBN (13): 978-84-939843-6-6, pp. 848–854, Dec. 2012.

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