Among the new super-resolution technologies, stimulated emission depletion (STED) is the most versatile concept. It delivers immediate results: no time-consuming computation is necessary and the image is generated by a single reading. Therefore, super-resolution imaging from living samples requires STED. Furthermore, many fluorescence probes are applicable for STED. And in combination with the white laser, there is nothing left to be desired. Whether the sample is tissue, e.g. muscle striation details, classical cytoskeletons, nuclear proteins, yeast or bacterial details: STED serves for understanding structure and function by showing the very finest details.