GSD

Webinar: From Epifluorescence to Super-Resolution in 3D



This webinar will illustrate results obtained by biochemical, Epifluorescence, TIRFM, Confocal and GSD techniques. Depending on the aim of experimental question, different imaging techniques deliver insights into varying aspects of intracellular pathways. To achieve "True-to-detail imaging" of the spatial arrangement of proteins and other biomolecules in cells, GSDIM achieves resolutions up to 20 nm in x and y direction – beyond the diffraction limit of light microscopy. But Super-resolution microscopy can be applied in the axial (z-) direction, too. A recent commercial implementation of the astigmatism approach will be discussed in more detail during this webinar.



Super-Resolution

May 14, 2013

Webinar

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STED and GSDIM: Diffraction Unlimited Resolution for all Types of Fluorescence Imaging



This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning microscopy – usually an extension of a confocal microscope. Ground state depletion imaging (GSDIM) is a parallel recording widefield approach that explores inherent switching of fluorochromes and typically comes with a TIRF microscope. The two methods use very different approaches to reach the same goal: to see more details in light microscopes than possible when diffraction limited.



Super-Resolution

Mar 01, 2013

Abstract

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Webinar: From Live Cell Imaging to Super-Resolution



The beauty of cells, magnified and resolved by light microscopy, has been fascinating investigators since the 19th century. Today, functional research in living cells is often a prerequisite for biological studies. And keeping the cells close to the necessary conditions whilst under microscopical observation is key. In this webinar, Dr. Christoph Bauer will share his experiences of live cell imaging and talk about how the microscopy challenges, such as optical aberrations at 37°C or focus drift in long-term experiments, can be addressed.



Super-Resolution

Jan 29, 2013

Webinar

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Tubulin Modifications Affect Monolayer Formation and Apical Trafficking in Epithelial Cells



The development and maintenance of polarized epithelial cells requires the establishment of complicated subcellular machinery. We studied the role of post-translational tubulin modifications within this process. At first, the distribution of detyrosinated microtubules was assessed in MDCK cells via immunofluorescence microscopy. No preferential accumulation of tyrosinated or detyrosinated microtubules could be detected at the apical or basal cell poles in epithelial cell cysts. However, during monolayer formation, the quantities of detyrosinated tubulin increased significantly over time.



Super-Resolution

Dec 05, 2012

Article

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Abstracts of the 2nd European Super-Resolution User-Club Meeting



The 2nd meeting of the Leica Super-resolution User club was held from September 25 to 27, 2012 in collaboration with the Science for Life Laboratory at the Karolinska Institute, Stockholm, Sweden. With a mixture of engaging talks by key experts in the field of super-resolution microscopy and stimulating discussion sessions, the meeting proved as popular as last year’s event, attracting a wide range of scientists interested in both confocal and widefield super-resolution and sample preparation techniques.



Super-Resolution

Nov 02, 2012

Article

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Sample Preparation for GSDIM



The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as 20 nanometers. GSDIM is suitable for a wide range of samples.



Super-Resolution

Jul 27, 2012

Tutorial

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Widefield Super-Resolution with GSDIM



Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes.



Super-Resolution

Jul 12, 2012

Tutorial

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Webinar: Beyond the Diffraction Limit



Super-resolution imaging is expanding our understanding of cellular structures and protein dynamics. First suggested more than 20 years ago, fluorescence techniques capable of resolution in the tens of nanometer range are becoming increasingly commonplace today. In this special web seminar, viewers will learn the basic principles behind several of the most commonly used super-resolution imaging techniques (STED, PALM, STORM, and GSD), hear specific examples where super-resolution approaches have provided unique biological insights, and get a glimpse of what the future holds for super-resolution studies.



Super-Resolution

Mar 28, 2012

Webinar

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Video Tutorial: How to Optimize Sample Preparation for GSD Microscopy



This video tutorial presents a reliable way to produce samples for super-resolution GSD imaging with a special focus on the mounting step. Stable and flat mounting of the coverslip increases the performance of the overall system, leading to an improved resolution of the GSD image.



Super-Resolution

Jan 13, 2012

Tutorial

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Webinar: Applications, Labeling Strategies and Fluorophores for Super-Resolution



The free online webinar on super-resolution presented by Leica Microsystems in association with Microscopy & Analysis took place on Tuesday, 15 November 2011. Register and view the webinar on demand.

Nov 15, 2011

Webinar

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How Widefield Super-Resolution GSDIM Images are Created



The localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM.



Super-Resolution

Oct 31, 2011

Tutorial

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Step by Step Guide to the Molecular Basics of GSDIM Microscopy



Ground state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a diffraction-limited setup) has to be broken up. Individual fluorophores must be temporally "separated" to allow high precision detection of single molecules. This tutorial will explain the molecular basics of GSDIM.



Super-Resolution

Oct 28, 2011

Tutorial

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Super-Resolution GSDIM Microscopy



The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within the cell. There is now a first commercial system (the Leica SR GSD) on the market that is helping to make the GSDIM technique available to a wider group of users in research labs and imaging centers.



Super-Resolution

Oct 21, 2011

Article

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Abstracts of the First European Super-Resolution User Club Meeting



The first European Super-resolution User Club meeting took place from October 27 to 29 in Göttingen, Germany. Prof. Stefan Hell, the inventor of the STED technology, has hosted this first meeting. The user club is aimed at pioneering researchers from the European scientific community, who are early adopters and developers of super-resolution techniques.



Super-Resolution

Oct 11, 2011

Abstract

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