Leica Science Labby Leica Microsystems

Interactive Tutorial

    The Principles of Polarization Contrast

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    Polarization contrast microscopy is a convenient way to make birefringent crystalline structures like starch grains or cellulose visible without staining. This tutorial will explain the optical elements in the light path and the operating mode of polarization contrast taking the example of an inverted and motorized high-end research light microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.
    Basics in Microscopy
    Basics in Microscopy
    Nov 26, 2012
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    Principles of Multiphoton Microscopy for Deep Tissue Imaging

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    This interactive tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking advantage of the reduced scattering of longer wavelengths. This makes multiphoton imaging the perfect tool for deep tissue imaging in thick sections and living animals.
    Multiphoton Microscopy
    Multiphoton Microscopy
    Aug 24, 2012
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    The Principles of Modulation Contrast

    Intro_Modulation_Contrast_Tutorial_03.jpg
    Modulation contrast creates 3D-like images of unstained specimens, rendering it the contrasting method of choice for applications like e.g. in vitro fertilization, where DIC is not possible (due to the usage of plastics) or phase contrast does not deliver satisfying results. This tutorial explains the optical elements in the light path and the operating mode of the modulation contrast.
    Basics in Microscopy
    Basics in Microscopy
    Jun 25, 2012
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    The Principles of Phase Contrast

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    In this tutorial the principle of phase contrast imaging is described taking the example of an inverted research microscope. Additionally, the alignment of the components needed for phase contrast is shown in the interactive part of the tutorial.
    Basics in Microscopy
    Basics in Microscopy
    Jun 01, 2012
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    Depletion and Emission Beam Paths in STED Microscopy

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    STED is one technology, and it requires already additional beam-routings as compared to pure fluorescence imaging. The Leica TCS SP5 offers configurations with STED, confocal and multiphoton imaging in the very same instrument. This unique concept unites technologies of super-resolution fluorescence, multichannel confocal fluorescence, multiphoton-excited fluorescence and second-harmonic or higher order nonlinear image generation. The various beam-paths are shown and explained in this tutorial.
    Super-Resolution
    Super-Resolution
    May 21, 2012
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    Stimulated Emission Depletion (STED)

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    Among the new super-resolution technologies, stimulated emission depletion (STED) is the most versatile concept. Whether the sample is tissue, e.g. muscle striation details, classical cytoskeletons, nuclear proteins, yeast or bacterial details: STED serves for understanding structure and function by showing the very finest details.
    Super-Resolution
    Super-Resolution
    May 21, 2012
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    Beam Paths of a Continuous Wave STED Microscope

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    This tutorial gives on overview of the excitation of an detection light path with a continuous wave (CW) STED microscope.
    Super-Resolution
    Super-Resolution
    May 14, 2012
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    STED Super-Resolution Microscopy

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    This tutorial focusses on the principles of STED super-resolution microscopy. The underlying photo physical processes are explained - e.g. with the help of a Jablonski diagram - and the integration into a confocal laser scanning microscope.
    Super-Resolution
    Super-Resolution
    May 02, 2012
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    Differential Interference Contrast

    Intro-DIC_Tutorial_04.jpg
    The examination of live unstained biological specimens often suffers from poor contrast and therefore bad visibility of the specimen. Thick specimens in particular, such as brain slices, show up as nothing more than light grey structures instead of single cells. This tutorial explains the optical elements in the light path and the operating mode of DIC (differential interference contrast) on the example of an inverted and motorized high-end research light microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.
    Live-Cell Imaging
    Live-Cell Imaging
    Jan 30, 2012
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    Step by Step Guide to the Molecular Basics of CARS Microscopy

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    CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering.
    CARS Microscopy
    CARS Microscopy
    Nov 01, 2011
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    How Widefield Super-Resolution GSDIM Images are Created

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    The localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM.
    Super-Resolution
    Super-Resolution
    Oct 31, 2011
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    Step by Step Guide to the Molecular Basics of GSDIM Microscopy

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    Ground state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a diffraction-limited setup) has to be broken up. Individual fluorophores must be temporally "separated" to allow high precision detection of single molecules. This tutorial will explain the molecular basics of GSDIM.
    Super-Resolution
    Super-Resolution
    Oct 28, 2011
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    Step by Step Guide to Hybrid Detection and Photon Counting

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    This tutorial explains the underlying hybrid detection technology and compares it to photomultiplier technology. The implications of hybrid detection design for imaging and photon counting are discussed. The tutorial closes with a brief summary of photon counting in the context of imaging.
    Live-Cell Imaging
    Live-Cell Imaging
    Oct 20, 2011
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    Koehler Illumination

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    This tutorial demonstrates the basic steps for setting up a correct illumination of specimen with transmitted (and reflected) light.
    Basics in Microscopy
    Basics in Microscopy
    May 31, 2011
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