Live Cell Imaging

Imaging Enzymes at Work

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For the understanding of functions of proteins in biological and pathological processes, reporter molecules such as fluorescent proteins have become indispensable tools for visualizing the location of these proteins in intact animals, tissues, and cells. For enzymes, imaging their activity also provides information on their function or functions, which does not necessarily correlate with their location. Metabolic mapping enables imaging of activity of enzymes.

Live-Cell Imaging

Apr 05, 2013

Abstract

Intravital Immunofluorescence for Visualizing the Microcirculatory and Immune Microenvironments in the Mouse Ear Dermis

Intravital imaging of inflammatory and remodeling processes has been an important area of research and has also motivated the creation of numerous transgenic reporter mouse models that express fluorescent proteins. Our paper (Kilarski, Güç et al. 2013) describes a new in vivo imaging technique, which design was based on innovative concept of using immunostaining for live cells and tissue structures on surgically exposed mouse dermis without causing harmful immunotoxic effect.

Live-Cell Imaging

Mar 19, 2013

Article

Webinar: From Live Cell Imaging to Super-Resolution

The beauty of cells, magnified and resolved by light microscopy, has been fascinating investigators since the 19th century. Today, functional research in living cells is often a prerequisite for biological studies. And keeping the cells close to the necessary conditions whilst under microscopical observation is key. In this webinar, Dr. Christoph Bauer will share his experiences of live cell imaging and talk about how the microscopy challenges, such as optical aberrations at 37°C or focus drift in long-term experiments, can be addressed.

Super-Resolution

Jan 29, 2013

Webinar

Webinar: Challenges in Live Cell Imaging

Live cell imaging is one tool in the cell biologist's tool box. There are, however, a variety of technologies and methods to achieve this. In this webinar, three practicing researchers discuss: Their use of live cell imaging and how it has helped them achieve publishable results.

Live-Cell Imaging

Oct 10, 2012

Webinar

Digital Camera Technologies for Scientific Bio-Imaging

This four-part series of articles published in Microscopy and Analysis covers the factors to consider in choosing a camera among CCD, EMCCD, and scientific-grade CMOS camera technologies for biological imaging applications. The differences among the sensor architectures and the impact of parameters such as pixel size, noise, and QE on signal-to-noise performance, image quality, and Nyquist sampling are considered.

Fluorescence Microscopy

Jul 19, 2012

Abstract

Webinar: Techniques and Methods in Live-Cell Imaging

Imaging technologies are ubiquitous in today's life science laboratory. From basic microscopy to high throughput modalities, most cell based research benefits from some tried and true methods for imaging.

Jul 18, 2012

Webinar

Webinar: Introducing the New Leica TCS SP8 Confocal Platform

Discover Ultra-detailed Imaging with the Leica TCS SP8 ...

Confocal Microscopy

Jun 21, 2012

Webinar

Webinar: Live Cell Imaging

Cells communicate and interact with one another to transmit signals and initiate reactions that facilitate coordinated events. These interactions are critical in almost every aspect of physiology, from transmitting neuronal signals that allow us to sense hot and cold to initiating immune responses that fight against infection.

May 24, 2012

Webinar

Stimulated Emission Depletion (STED)

Among the new super-resolution technologies, stimulated emission depletion (STED) is the most versatile concept. Whether the sample is tissue, e.g. muscle striation details, classical cytoskeletons, nuclear proteins, yeast or bacterial details: STED serves for understanding structure and function by showing the very finest details.

Super-Resolution

May 21, 2012

Tutorial

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the evanescent wave, which arises from the glass/water or glass/specimen interface, is that its propagation in z-direction gradually degrades, limiting its penetration depth into the specimen to some hundred nanometers.

TIRF Microscopy

Apr 25, 2012

Tutorial

Live-Cell Imaging

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The understanding of complex and fast cellular dynamics is an important step to get insight into biological processes. Therefore, today’s life science research more and more demands studying physiological events on the molecular level in real-time.

Live-Cell Imaging

Apr 04, 2012

Tutorial

Live-cell Imaging Techniques

The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic processes like cell migration, morphological changes of cells, organs or whole animals and physiological (e.g. changes of intracellular ion composition) events in living specimens in real time.

Live-Cell Imaging

Feb 24, 2012

Tutorial

The New Repository on the Block

The need for data validation and accessibility has never been greater than it is today. We are inundated with information from a multitude of resources, but how can we easily evaluate the accuracy of that data? In the past, the peer review process provided this and was often run by publishers.

Fluorescence Microscopy

Feb 07, 2012

Article

Ratiometric Imaging

Many fundamental functions of a cell strongly depend on delicate, but nevertheless dynamic balances of ions (e.g. calcium, magnesium), voltage potentials and pH between the cell’s cytosol and the surrounding extracellular space. Ratiometric imaging allows reliable estimations of ion concentrations and pH or voltage changes by measuring fluorophore emission shifts.

Live-Cell Imaging

Dec 12, 2011

Tutorial

Ratiometric Imaging Needs a Specialized Setup

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Ratiometric imaging is widely used to study highly dynamic intracellular ion, voltage or pH changes. The most common application, however, is calcium imaging. Ratiometric imaging is also used for investigating cellular networks, where e.g. relative calcium concentrations are passed among cells or different cell types dynamically change ion, voltage or pH levels. Also FRET assays can considerably benefit from ratiometric imaging, as the signal-to-noise-ratio is greatly improved.

Live-Cell Imaging

Dec 12, 2011

Tutorial

The Patch-Clamp Technique

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Especially in neuroscience, the physiology of ion channels has always been a major topic of interest. The development of the patch-clamp technique in the late 1970s has given electrophysiologists new prospects. It allows high-resolution current recordings not only of whole cells, but also of excised cellular patches. Even single-channel opening events can be investigated. However, with its complex technical, physical and biological background, the need for highly sensitive equipment and the huge amount of skills required of the experimenter, electrophysiology is still one of the most challenging methods in daily laboratory work.

Nov 09, 2011

Tutorial

Step by Step Guide to the Molecular Basics of CARS Microscopy

CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering.

CARS Microscopy

Nov 01, 2011

Tutorial

Step by Step Guide to Hybrid Detection and Photon Counting

This tutorial explains the underlying hybrid detection technology and compares it to photomultiplier technology. The implications of hybrid detection design for imaging and photon counting are discussed. The tutorial closes with a brief summary of photon counting in the context of imaging.

Live-Cell Imaging

Oct 20, 2011

Tutorial

Abstracts of the First European Super-Resolution User Club Meeting

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The first European Super-resolution User Club meeting took place from October 27 to 29 in Göttingen, Germany. Prof. Stefan Hell, the inventor of the STED technology, has hosted this first meeting. The user club is aimed at pioneering researchers from the European scientific community, who are early adopters and developers of super-resolution techniques.

Super-Resolution

Oct 11, 2011

Abstract

Optical Contrast Methods

Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the specimen into amplitude shifts that are visible to the human eye as differences in brightness.

Basics in Microscopy

Jun 10, 2011

Tutorial

Webinar: Principles and Applications of  Live Cell Imaging

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Live cell imaging has become a cornerstone of modern cell biology, as well as a technique routinely employed in other fields such as developmental biology, stem cell biology and neuroscience. In this webinar, you will learn about the effective use of live cell imaging for a growing number of applications.

Live-Cell Imaging

Apr 27, 2011

Webinar

TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional fluorescence microscopy images. Hence, the approach reveals new insights into the composition of this characteristic cell pole that elucidate processes in apical protein trafficking.

Live-Cell Imaging

Apr 13, 2011

Article

Sniffing Out the Secrets of Social Behavior

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Yet we are only just beginning to understand the complexities and functional differences of the sense of smell in mammals. Prof. Marc Spehr, head of the Department of Chemosensation at RWTH Aachen University since 2009, explains his findings on the neuronal mechanisms of olfactory perception and signal processing using the mouse model. He and his team are trying to find out how substances for social interaction are perceived and how this perception generates a specific type of behavior.

Live-Cell Imaging

Nov 02, 2010

Article

FRET Sensitized Emission Wizard Widefield

Quantitative Fluorescence

Apr 15, 2009

Tutorial

New Standard in Electrophysiology and Deep Tissue Imaging

Mar 17, 2009

Article

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