LMD

Laser Microdissection Publication List



This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research.



Laser Microdissection

May 14, 2013

Article

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Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome"



The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection.



Laser Microdissection

Nov 09, 2012

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K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration



Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a synaptically triggered switch from low-frequency background spiking to transient high-frequency burst firing. We found that, in medial DA neurons of the substantia nigra (SN), activity of ATP-sensitive potassium (K-ATP) channels enabled NMDA-mediated bursting in vitro as well as spontaneous in vivo burst firing in anesthetized mice.



Laser Microdissection

Oct 05, 2012

Abstract

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Application-specific Consumables for Laser Microdissection



There are many different types of consumables for laser microdissection instruments. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own individual configuration whatever their field of research is. The most common consumables are various glass membrane slides in combination with standard caps or cap strips to collect the dissectate.



Laser Microdissection

Aug 30, 2012

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Laser Microdissection Combined with AmpliGrid/LOC Devices: High-Throughput for Efficient Screening



LOC technology enables complex biochemical and physical processes to run in a small space using a minimum of chemicals. Generally, LOC is a minimal device in the form of a portable quick test. This frequently capillary-based microfluidic system is normally accommodated on a substrate no larger than a chip card.



Laser Microdissection

Feb 27, 2012

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Region-Specific Gene Expression in Adult Mouse CNS Tissues



Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA expression in the adult mouse retina and hippocampus.



Laser Microdissection

Feb 22, 2012

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TED Talk of Allan Jones: Mapping the Human Brain



How can we begin to understand the complexity of the human brain and the way it works? The same way we begin to understand a city: by making a map. In this talk, Allan Jones shows how his team is mapping which genes are turned on in each tiny region, and how it all connects up.



Neuroscience

Dec 02, 2011

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Cell Cultures and Laser Microdissection



Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc. would not be possible without live cell cultures. Allowing morphological and biochemical observations of single cells under different experimental conditions, they provide a unique source of information.



Laser Microdissection

Oct 07, 2011

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An Introduction to Laser Microdissection



Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples and tissue using a focused laser beam.



Laser Microdissection

Jun 28, 2011

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Applications of Laser Microdissection



Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms.



Laser Microdissection

Jun 27, 2011

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Users Report on the Relevance of Laser Microdissection for Their Research Results



Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection.



Laser Microdissection

Apr 13, 2011

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The Morbus Parkinson Puzzle



A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson.



Neuroscience

Nov 02, 2010

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The Mitochondrial Hypothesis of Ageing



Why do we grow old? Research scientists have been looking for an answer to this question for many years – particularly against the background of the increase in neurodegenerative diseases among older people such as Morbus Parkinson.



Neuroscience

Sep 07, 2009

Article

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