Two-Photon Imaging

Webinar: Confocal and Two-Photon Microscopy Methods for Imaging the Brain

A deeper understanding of brain function requires visualization of the complex architecture of neurons and their connections. In this special webinar, two researchers working at the cutting-edge of brain imaging will discuss their applications of the latest confocal and two-photon microscopy techniques. From fluorescent labeling and imaging of neural circuits using the Brainbow system to in vivo imaging of brain structure, function, and blood flow, this webinar will give researchers a deeper appreciation of the potential for new microscopy methods to unlock the secrets of the cellular world. Unique insights into sample handling and processing of multicolor images will also be presented, and attendees will have the opportunity to ask questions.

Confocal Microscopy

May 08, 2013

Webinar

Developments in Multiphoton Excitation Microscopy

Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom or molecule reported in the 1930s in her PhD dissertation thesis, we can state that multiphoton excitation (MPE) microscopy, more frequently identified with two-photon excitation (2PE) fluorescence microscopy, is a key microscopy method in many areas from medicine to biology, from biophysics to materials science.

Multiphoton Microscopy

Oct 08, 2012

Abstract

Principles of Multiphoton Microscopy for Deep Tissue Imaging

This interactive tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking advantage of the reduced scattering of longer wavelengths. This makes multiphoton imaging the perfect tool for deep tissue imaging in thick sections and living animals.

Multiphoton Microscopy

Aug 24, 2012

Tutorial

CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet molecular specific information can be obtained from a range of important bio-molecular compounds.

CARS Microscopy

Jul 17, 2012

Article

Modern Fluorescent Proteins and their Biological Applications

Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. The second article focuses on novel monomeric RFPs and their application for studying gene expression, nuclear localization, and dynamics using advanced imaging.

Fluorescence Microscopy

Nov 18, 2011

Abstract

Abstracts of the First European Super-Resolution User Club Meeting

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The first European Super-resolution User Club meeting took place from October 27 to 29 in Göttingen, Germany. Prof. Stefan Hell, the inventor of the STED technology, has hosted this first meeting. The user club is aimed at pioneering researchers from the European scientific community, who are early adopters and developers of super-resolution techniques.

Super-Resolution

Oct 11, 2011

Abstract

Good Vibrations

In recent years, new molecular imaging techniques, such as coherent anti-Stokes Raman scattering microscopy (CARS), have been developed for rapid vibrational imaging of living cells.

Multiphoton Microscopy

Apr 13, 2011

Article

CARS and Confocal

The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is non-toxic, non-destructive, and minimally invasive.

Multiphoton Microscopy

Nov 02, 2010

Tutorial

Exploring the Concert of Neuronal Activities

Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic interconnections can be visualized.

Confocal Microscopy

Nov 02, 2010

Article

What is an OPO?

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Because light scattering is dependent on the wavelength, better tissue penetration can be achieved by using longer excitation wavelengths. This is where excitation with infrared light, two-photon processes, and the OPO (optical parameter oscillator) can dramatically improve image quality.

Confocal Microscopy

Nov 02, 2010

Article

Deep Tissue Imaging

To gain new insight into the fundamental control of cell response to physical changes and to study the dynamics and roles of biological flow during the development of the zebrafish, Dr. Julien Vermot established his lab last year at the Institute of Genetics and Molecular and Cellular Biology (IGBMC) in Strasbourg, France.

Multiphoton Microscopy

Nov 02, 2010

Article

Stem Cell Biology in Cancer Research

The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells.

Multiphoton Microscopy

Apr 23, 2010

Article

From Molecules to Tissues

Sequencing of the human genome stimulated a radical change in the approach to biomedical research. The comprehension of the mechanisms regulating life gained a scale-up in throughput to speed up the retrieval of data for a global vision of a system of incomparable complexity.

Multiphoton Microscopy

Aug 22, 2008

Article

4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy

We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. Two-photon photoactivation produces an effective real three-dimensional (3D) localization of the molecular switching of pa-GFP in the bright state.

Confocal Microscopy

Oct 15, 2006

Article

Multi-photon Excitation Microscopy

Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science where imaging can be coupled to the possibility of using the microscopes in an active way, too.

Multiphoton Microscopy

Jun 06, 2006

Abstract

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