http://www.leica-microsystems.com/science-lab/tag/tags/photo-activatable-proteins/show/tag/ Article tagged with Photoactivation en-US The term "Highlighter Proteins" combines a group of genetically altered fluorescent proteins with very special attributes: They can be activated from a non fluorescent state, they can change their emission spectrum or they are even able to be switched "on and off" for a lot of times. This behavior is due to some distinct photo physical attributes. At the single molecule level many fluorescent proteins show an intrinsic blinking activity which can be influenced by external illumination. http://www.leica-microsystems.com/science-lab/highlighter-proteins-and-fluorescent-timers/ Wed, 20 Jun 2012 00:00:00 +0200 Dr. Christoph Greb The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic processes like cell migration, morphological changes of cells, organs or whole animals and physiological (e.g. changes of intracellular ion composition) events in living specimens in real time. http://www.leica-microsystems.com/science-lab/live-cell-imaging-techniques/ Fri, 24 Feb 2012 00:00:00 +0100 Dr. Zhongxiang Jiang, Dr. Thomas Veitinger Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. The second article focuses on novel monomeric RFPs and their application for studying gene expression, nuclear localization, and dynamics using advanced imaging. http://www.leica-microsystems.com/science-lab/modern-fluorescent-proteins-and-their-biological-applications/ Fri, 18 Nov 2011 13:50:00 +0100 Robert H. Singer, Olesya V. Stepanenko, Olga V. Stepanenko, Daria M. Shcherbakova, Irina M. Kuznetsova, Konstantin K. Turoverov, Bin Wu, Kiryl D. Piatkevich, Timothée Lionnet, Prof. Vladislav Verkhusha The comprehension of stem cell biology and its molecular basis is now acquiring paramount importance in cancer research. The need to look at a single, possibly living, cell makes fluorescence microscopy and confocal microscopy invaluable allies in the study of stem cells. http://www.leica-microsystems.com/science-lab/stem-cell-biology-in-cancer-research/ Fri, 23 Apr 2010 00:00:00 +0200 Mario Faretta, Prof. Alberto Diaspro, Paolo Bianchini, Isabella Ponzanelli, Ilaria Rancati, Angelo Cicalese, Laura Furia We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. Two-photon photoactivation produces an effective real three-dimensional (3D) localization of the molecular switching of pa-GFP in the bright state. http://www.leica-microsystems.com/science-lab/4d-photoactivation-of-pa-gfp-in-living-cells-using-two-photon-excitation-laser-scanning-microscopy/ Sun, 15 Oct 2006 00:00:00 +0200 Mario Faretta, Illaria Testa, Valentina Caorsi, Davide Mazza, Giuseppe Vicidomini, Sara Barozzi, Dario Parazzoli, Pietro Transidico, Massimiliano Garrè, Prof. Alberto Diaspro