http://www.leica-microsystems.com/science-lab/tag/tags/tubulin/show/tag/ Article tagged with Tubulin en-US The development and maintenance of polarized epithelial cells requires the establishment of complicated subcellular machinery. We studied the role of post-translational tubulin modifications within this process. At first, the distribution of detyrosinated microtubules was assessed in MDCK cells via immunofluorescence microscopy. No preferential accumulation of tyrosinated or detyrosinated microtubules could be detected at the apical or basal cell poles in epithelial cell cysts. However, during monolayer formation, the quantities of detyrosinated tubulin increased significantly over time. http://www.leica-microsystems.com/science-lab/tubulin-modifications-affect-monolayer-formation-and-apical-trafficking-in-epithelial-cells/ Wed, 05 Dec 2012 00:00:00 +0100 Prof. Ralf Jacob, Dr. Sabrina Zink Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction encountered with standard confocal laser scanning microscopes and conventional far-field optical microscopes[1]. Compared to traditional confocal microscopy, STED offers exceptional improvements in resolution allowing visualization of cellular events at unprecedented levels. http://www.leica-microsystems.com/science-lab/subcellular-localization-of-akt-and-tubulin-using-super-resolution-microscopy/ Thu, 01 Dec 2011 00:00:00 +0100 Ph.D. Carl A. Ascoli, Ph.D. Myriam Gastard, Ph.D. David P. Chimento