TIRF Publication List

June 02, 2014

Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm.

The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or lasers, the evanescent field only penetrates the specimen by about 100 nm starting from the coverslip/medium interface. This allows "additive" optical sectioning of the specimen, starting at the coverslip at a very high axial resolution, and brings several advantages for specific applications such as the observation of membrane-associated processes.

This monthly updated references list presents current papers in the major application fields for TIRF microscopy.

Publications Leica AM TIRF MC

Expression of Nucleolin Affects Microtubule DynamicsGaume X Place C Delage H Mongelard F Monier K et. al.PLOS ONE2016 vol: 11 (6) pp: e0157534

RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
Kajiho H Kajiho Y Frittoli E Confalonieri S Bertalot G et. al.
EMBO reports
2016 vol: 17 (7) pp: 1061-80

Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments
Behrens J Solga R Ziemann A Rastetter R Berwanger C et. al.
European Journal of Cell Biology
2016

Driven Translocation of Polynucleotides Through an Aerolysin Nanopore
Cao C Yu J Wang Y Ying Y Long Y et. al.
Analytical Chemistry
2016 vol: 88 (10) pp: 5046-5049

Aurora A drives early signalling and vesicle dynamics during T-cell activation
Blas-Rus N Bustos-Morán E Pérez de Castro I de Cárcer G Borroto A et. al.
Nature Communications
2016 vol: 7 pp: 11389

Morphotype-specific effector functions of Cryptococcus neoformans PUM1
Kaur J Panepinto J
Scientific reports
2016 vol: 6 pp: 23638

In Vitro Investigation of Influences of Chitosan Nanoparticles on Fluorescein Permeation into Alveolar Macrophages
Chachuli S Nawaz A Shah K Naharudin I Wong T et. al.
Pharmaceutical Research
2016 vol: 33 (6) pp: 1497-1508

HDAC6 regulates the dynamics of lytic granules in cytotoxic T lymphocytes
Núñez-Andrade N Iborra S Trullo A Moreno-Gonzalo O Calvo E et. al.
Journal of cell science
2016 vol: 129 (7) pp: 1305-11

Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ α-1) and profilin 2
Rollason R Wherlock M Heath J Heesom K Saleem M et. al.
Bioscience reports
2016 vol: 36 (1) pp: e00302

Nuclear trapping through inhibition of exosomal export by indomethacin increases cytostatic efficacy of doxorubicin and pixantrone
Koch, Raphael;  Aung, Thiha;  Vogel, Daniel;  Chapuy, Bjoern;  Wenzel, Dirk et al. (2015)
Clin. Cancer Res. p. 1078-0432.CCR-15-0577-

Insulin Regulates the Activity of the High-Affinity Choline Transporter CHT
Fishwick, Katherine J.;  Rylett, R. Jane (2015)
PLOS ONE vol. 10 (7) p. e0132934

Regions outside of conserved PxxPxR motifs drive the high affinity interaction of GRB2 with SH3 domain ligands
Bartelt, Rebekah R.;  Light, Jonathan;  Vacaflores, Aldo;  Butcher, Alayna;  Pandian, Madhana et al. (2015)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research vol. 1853 (10) p. 2560-2569

A STIM2 splice variant negatively regulates store-operated calcium entry
Miederer, Anna-Maria;  Alansary, Dalia;  Schwär, Gertrud;  Lee, Po-Hsien;  Jung, Martin et al. (2015)
Nature Communications vol. 6

Connection between the conformation and emission properties of poly[2-methoxy-5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene] single molecules during thermal annealing
Ou, Jiemei;  Yang, Yuzhao;  Lin, Wensheng;  Yuan, Zhongke;  Gan, Lin et al. (2015)
Applied Physics Letters    vol. 106 (12) p. 123304

Facilitation of Orai3 targeting and store-operated function by Orai1
Alansary, Dalia;  Bogeski, Ivan;  Niemeyer, Barbara A. (2015)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research vol. 1853 (7) p. 1541-1550

GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production
Bilal M Houtman J (2015)
Frontiers in Immunology vol: 6

Myosin VI deafness mutation prevents the initiation of processive runs on actin
Pylypenko O Song L Shima A Yang Z Houdusse A Sweeney H (2015)
Proceedings of the National Academy of Sciences vol: 112 (11) pp: E1201-E1209

PDK1 regulates focal adhesion disassembly by modulating endocytosis of αvβ3 integrin
di Blasio, Laura;  Gagliardi, Paolo Armando;  Puliafito, Alberto;  Sessa, Roberto;  Seano, Giorgio et al. (2015)
J. Cell Sci. vol. 128 (5) p. 863-877

Site specific phosphorylation of kindlin-3 regulates its capacity to control cellular responses mediated by integrin αIIbβ3
Bialkowska, Katarzyna;  Byzova, Tatiana V.;  Plow, Edward F. (2015)
J. Biol. Chem. p. M114.634436-

Multiplex single-molecule interaction profiling of DNA-barcoded proteins
Gu, Liangcai;  Li, Chao;  Aach, John;  Hill, David E;  Vidal, Marc et al. (2014)
Nature vol. 515 (7528)

PDZK1/NHERF3 Differentially Regulates Corticotropin-releasing Factor Receptor 1 and Serotonin 2A Receptor Signaling and Endocytosis
Walther, Cornelia;  Caetano, Fabiana A.;  Dunn, Henry A.;  Ferguson, Stephen S.G. (2015)
Cellular Signalling vol. 27 (3) p. 519-531

Membrane-cytoskeleton dynamics in the course of mast cell activation
Pavel Dráber, Petr Dráber (2015)
Methods in molecular biology 1220 p. 219-37

Multiplex single-molecule interaction profiling of DNA-barcoded proteins
Liangcai Gu, Chao Li, John Aach, David E. Hill, Marc Vidal, George M. Church (2014)
Nature 515 (7528) p. 554-557

Exosome poly-ubiquitin inhibits platelet activation, downregulates CD36 and inhibits pro-atherothombotic cellular functions
S Srikanthan, W Li, R L Silverstein, T M McIntyre (2014)
Journal of thrombosis and haemostasis : JTH 12 (11) p. 1906-17

PDK1-mediated activation of MRCKα regulates directional cell migration and lamellipodia retraction
Paolo Armando Gagliardi, Laura di Blasio, Alberto Puliafito, Giorgio Seano, Roberto Sessa, Federica Chianale, Thomas Leung, Federico Bussolino, Luca Primo (2014)
The Journal of cell biology 206 (3) p. 415-34

The large GTPase Mx1 is involved in apical transport of MDCK cells
Florian Hoff, Christoph Greb, Christina Hollmann, Ellena Hönig, Ralf Jacob (2014)
Traffic

Enhanced single molecule fluorescence of conjugated polymer poly(3-hexylthiophene) on silver-nanocubes
Jiemei Ou, Yuzhao Yang, Zhongke Yuan, Yu Zhang, Lin Gan, Xudong Chen (2014)
Synthetic Metals 195 p. 9-15

CLN3 Deficient Cells Display Defects in the ARF1-Cdc42 Pathway and Actin-Dependent Events
Mark L Schultz, Luis Tecedor, Colleen S Stein, Mark A Stamnes, Beverly L Davidson (2014)
PloS one 9 (5) p. e96647

Interactions of the protein-tyrosine phosphatase-α with the FAT domain of focal adhesion kinase are involved in IL-1 signaling in fibroblasts
Qin Wang, Yongqiang Wang, Dominik Fritz, Dhaarmini Rajshankar, Gregory P Downey, Christopher A McCulloch (2014)
The Journal of biological chemistry p. M113.540294-

Miro-1 links mitochondria and microtubule dynein motors to control lymphocyte migration and polarity
Giulia Morlino, Olga Barreiro, Francesc Baixauli, Javier Robles-Valero, José M González-Granado, Ricardo Villa-Bellosta, Jesús Cuenca, Carlos O Sánchez-Sorzano, Esteban Veiga, Noa B Martín-Cófreces, Francisco Sánchez-Madrid (2014)
Molecular and cellular biology p. MCB.01177-13-

Activated PLC-γ1 is catalytically induced at LAT but activated PLC-γ1 is localized at both LAT- and TCR-containing complexes
Noemi Cruz-Orcutt, Aldo Vacaflores, Sean F. Connolly, Stephen C. Bunnell, Jon C.D. Houtman (2014)
Cellular Signalling

Functional Cooperation of Metabotropic Adenosine and Glutamate Receptors Regulates Postsynaptic Plasticity in the Cerebellum
Y. Kamikubo, T. Shimomura, Y. Fujita, T. Tabata, T. Kashiyama, T. Sakurai, K. Fukurotani, M. Kano (2013)
Journal of Neuroscience 33 (47) p. 18661-18671

CLN3 Loss Disturbs Membrane Microdomain Properties and Protein Transport in Brain Endothelial Cells
Luis Tecedor, Colleen S Stein, Mark L Schultz, Hany Farwanah, Konrad Sandhoff, Beverly L Davidson (2013)
The Journal of neuroscience 33 (46) p. 18065-79

Focal Adhesion Kinase Negatively Regulates Lck Function Downstream of the T Cell Antigen Receptor
N. M. Chapman, S. F. Connolly, E. L. Reinl, J. C. D. Houtman (2013)
The Journal of Immunology p. 1301587

The GTPase-Activating Protein RN-tre Controls Focal Adhesion Turnover and Cell Migration
Andrea Palamidessi, Emanuela Frittoli, Nadia Ducano, Nina Offenhauser, Sara Sigismund, Hiroaki Kajiho, Dario Parazzoli, Amanda Oldani, Marco Gobbi, Guido Serini, Pier Paolo Di Fiore, Giorgio Scita, Letizia Lanzetti (2013)
Current Biology

TRPM7 triggers Ca2+ sparks and invadosome formation in neuroblastoma cells
Daan Visser, Michiel Langeslag, Katarzyna M. Kedziora, Jeffrey Klarenbeek, Alwin Kamermans, F. David Horgen, Andrea Fleig, Frank N. van Leeuwen, Kees Jalink (2013)
Cell Calcium

Seeded fibrillation as molecular basis of the species barrier in human prion diseases
Lars Luers, Oliver Bannach, Jan Stöhr, Michael Marius Wördehoff, Martin Wolff, Luitgard Nagel-Steger, Detlev Riesner, Dieter Willbold, Eva Birkmann (2013)
PloS one 8 (8) p. e72623

Dependence of Immunoglobulin Class Switch Recombination in B Cells on Vesicular Release of ATP and CD73 Ectonucleotidase Activity
Francesca Schena, Stefano Volpi, Caterina Elisa Faliti, Federica Penco, Spartaco Santi, Michele Proietti, Ursula Schenk, Gianluca Damonte, Annalisa Salis, Marta Bellotti, Franco Fais, Claudya Tenca, Marco Gattorno, Hermann Eibel, Marta Rizzi, Klaus Warnatz, Marco Idzko, Cemil Korcan Ayata, Mirzokhid Rakhmanov, Thierry Galli, Alberto Martini, Marco Canossa, Fabio Grassi, Elisabetta Traggiai (2013)
Cell Reports

Kindlin-1 Regulates Integrin Dynamics and Adhesion Turnover
Coert Margadant, Maaike Kreft, Giovanna Zambruno, Arnoud Sonnenberg (2013)
PLoS ONE 8 (6) p. e65341

PH-dependent recycling of galectin-3 at the apical membrane of epithelial cells
Tamara Straube, Tobias von Mach, Ellena Hönig, Christoph Greb, Dominik Schneider, Ralf Jacob (2013)
Traffic (Copenhagen, Denmark)

Uncoupling of mRNA synthesis and degradation impairs adaptation to host-temperature in Cryptococcus neoformans
Amanda L M Bloom, J T Graham Solomons, Virginia E Havel, John C Panepinto (2013)
Molecular microbiology

Barcoding cells using cell-surface programmable DNA-binding domains
Prashant Mali, John Aach, Je-Hyuk Lee, Daniel Levner, Lisa Nip, George M Church (2013)
Nature Methods

Spatial Coupling of JNK Activation to the B Cell Antigen Receptor by Tyrosine-Phosphorylated Ezrin
Neetha Parameswaran, Gospel Enyindah-Asonye, Nayer Bagheri, Neilay B Shah, Neetu Gupta (2013)
Journal of immunology p. jimmunol.1201292-

CD81 regulates cell migration through its association with Rac GTPase
Emilio Tejera, Vera Rocha-Perugini, Soraya López-Martín, Daniel Pérez-Hernández, Alexia I Bachir, Alan Rick Horwitz, Jesús Vázquez, Francisco Sánchez-Madrid, María Yáñez-Mo (2012)
Molecular biology of the cell p. mbc.E12-09-0642-

Mutations of the Ca2+-sensing Stromal Interaction Molecule STIM1 regulate Ca2+ influx by altered oligomerization of STIM1 and by destabilization of the Ca2+ channel Orai1
Tatiana Kilch, Dalia Alansary, Martin Peglow, Kathrin Doerr, Grigori Rychkov, Heiko Rieger, Christine Peinelt, Barbara A Niemeyer (2012)
The Journal of biological chemistry p. M112.417246-

End-binding protein 1 controls signal propagation from the T cell receptor
Noa B Martín-Cófreces, Francesc Baixauli, María J López, Diana Gil, Alicia Monjas, Balbino Alarcón, Francisco Sánchez-Madrid (2012)
The EMBO Journal

Total Internal Reflection Fluorescence (TIRF) Microscopy for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction
Ladan Parhamifar, S Moein Moghimi (2012)
Methods in molecular biology (Clifton, N.J.) 906 p. 473-82

TβRIII/β-arrestin2 regulates integrin α5β1 trafficking, function, and localization in epithelial cells
K Mythreye, E H Knelson, C E Gatza, M L Gatza, G C Blobe (2012)
Oncogene

STIM1 SAM domain mutants regulate CRAC by altering oligomerization and by destabilizing Orai1
T. Kilch, D. Al-Ansary, M. Peglow, G. Rychkov, H. Rieger, C. Peinelt and B. A. Niemeyer (2012)

Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin
Miriam Stoeber, Ina Karen Stoeck, Christine Hänni, Christopher Karl Ernst Bleck, Giuseppe Balistreri, Ari Helenius (2012)
The EMBO journal

The novel formin FMNL3 is a cytoskeletal regulator of angiogenesis.
Clare Hetheridge, Alice N Scott, Rajeeb K Swain, John W Copeland, Henry N Higgs, Roy Bicknell, Harry Mellor (2012)
Journal of cell science p. jcs.091066-

TI-VAMP/VAMP7 is the snare of secretory lysosomes contributing to ATP secretion from astrocytes
Claudia Verderio, Cinzia Cagnoli, Matteo Bergami, Maura Francolini, Ursula Schenk, Alessio Colombo, Loredana Riganti, Carolina Frassoni, Emanuela Zuccaro, Lydia Danglot, Claire Wilhelm, Thierry Galli, Marco Canossa, Michela Matteoli (2011) oli
Biology of the Cell

Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength.
Ramona Bhattacharya, Annette M Gonzalez, Phillip J Debiase, Humberto E Trejo, Robert D Goldman, Frederick W Flitney, Jonathan C R Jones (2009)
Journal of cell science 122 (Pt 9) p. 1390-400

Localization imaging using blinking quantum dots.
Fan-Ching Chien, Chiung Wen Kuo, Peilin Chen (2011)
The Analyst 136 (8) p. 1608-13

Ascorbate and endocytosed Motexafin gadolinium induce lysosomal rupture
Carsten Berndt, Tino Kurz, Sarah Bannenberg, Ralf Jacob, Arne Holmgren and Ulf T. Brunk
Cancer Letters, Volume 307, Issue 2, 28 August 2011, Pages 119-123, doi:10.1016/j.canlet.2011.03.023

Synthetic Protein Targeting by the Intrinsic Biorecognition Functionality of Poly(ethylene glycol) Using PEG Antibodies as Biohybrid Molecular Adaptors.
Janne T Hyotyla, Jie Deng, Roderick Y H Lim (2011)
ACS nano 5 (6) p. 5180-7

Exploring the Formation of Focal Adhesions on Patterned Surfaces Using Super-Resolution Imaging.
Fan-Ching Chien, Chiung Wen Kuo, Zong-Han Yang, Di-Yen Chueh, Peilin Chen (2011)
Small (Weinheim an der Bergstrasse, Germany) 7 (20) p. 2906-13

Trafficking of galectin-3 through endosomal organelles of polarized and non-polarized cells
Dominik Schneider, Christoph Greb, Annett Koch, Tamara Straube, Alexandra Ellia, Delphine Delacour and Ralf Jacob
European Journal of Cell Biology, Volume 89, Issue 11, November 2010, Pages 788-798, doi:10.1016/j.ejcb.2010.07.001

Photoswitching of E222Q GFP mutants: "concerted" mechanism of chromophore isomerization and protonation.
Stefania Abbruzzetti, Ranieri Bizzarri, Stefano Luin, Riccardo Nifosì, Barbara Storti, Cristiano Viappiani, Fabio Beltram (2010)
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 9 (10) p. 1307-19

Autoimmune kidney disease and impaired engulfment of apoptotic cells in mice with macrophage peroxisome proliferator-activated receptor gamma or retinoid X receptor alpha deficiency.
Tamás Roszer, María P Menéndez-Gutiérrez, Martina I Lefterova, Daniel Alameda, Vanessa Núñez, Mitchell A Lazar, Thierry Fischer, Mercedes Ricote (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (1) p. 621-31

A microfluidic imaging chamber for the direct observation of chemotactic transmigration.
Mark T Breckenridge, Thomas T Egelhoff, Harihara Baskaran (2010)
Biomedical microdevices 12 (3) p. 543-53

Vinculin nucleates actin polymerization and modifies actin filament structure.
Kuo-Kuang Wen, Peter A Rubenstein, Kris A DeMali (2009)
The Journal of biological chemistry 284 (44) p. 30463-73

The mitochondrial fission factor dynamin-related protein 1 modulates T-cell receptor signalling at the immune synapse.
Francesc Baixauli, Noa B Martín-Cófreces, Giulia Morlino, Yolanda R Carrasco, Carmen Calabia-Linares, Esteban Veiga, Juan M Serrador, Francisco Sánchez-Madrid (2011)
The EMBO journal 30 (7) p. 1238-50

Bi-modal regulation of a formin by srGAP2.
Frank M Mason, Ernest G Heimsath, Henry N Higgs, Scott H Soderling (2011)
The Journal of biological chemistry 286 (8) p. 6577-86

Targeted nuclear delivery using peptide-coated quantum dots.
Chiung-Wen Kuo, Di-Yen Chueh, Narendra Singh, Fan-Ching Chien, Peilin Chen (2011)
Bioconjugate chemistry 22 (6) p. 1073-80

Cargo binding induces dimerization of myosin VI.
Denis Phichith, Mirko Travaglia, Zhaohui Yang, Xiaoyan Liu, Alan B Zong, Daniel Safer, H Lee Sweeney (2009)
Proceedings of the National Academy of Sciences of the United States of America 106 (41) p. 17320-4

Ccr4 promotes resolution of the endoplasmic reticulum stress response during host temperature adaptation in Cryptococcus neoformans.
Virginia E Havel, Nathan K Wool, David Ayad, Kurtis M Downey, Christabel F Wilson, Peter Larsen, Julianne T Djordjevic, John C Panepinto (2011)
Eukaryotic cell 10 (7) p. 895-901

Phosphorylated filamin A regulates actin-linked caveolae dynamics.
Olivia Muriel, Asier Echarri, Christian Hellriegel, Dácil M Pavón, Leonardo Beccari, Miguel A Del Pozo (2011)
Journal of cell science 124 (Pt 16) p. 2763-76

Conformational switching in ezrin regulates morphological and cytoskeletal changes required for B cell chemotaxis.
Neetha Parameswaran, Ken Matsui, Neetu Gupta (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (7) p. 4088-97

Proteomics analysis of the ezrin interactome in B cells reveals a novel association with Myo18aa.
Ken Matsui, Neetha Parameswaran, Nayer Bagheri, Belinda Willard, Neetu Gupta (2011)
Journal of proteome research 10 (9) p. 3983-92

Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.
Davide Calebiro, Viacheslav O Nikolaev, Maria Cristina Gagliani, Tiziana de Filippis, Christian Dees, Carlo Tacchetti, Luca Persani, Martin J Lohse (2009)
PLoS biology 7 (8) p. e1000172

Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes.
Arnold Hayer, Miriam Stoeber, Christin Bissig, Ari Helenius (2010)
Traffic (Copenhagen, Denmark) 11 (3) p. 361-82

RON (MST1R) is a novel prognostic marker and therapeutic target for gastroesophageal adenocarcinoma.
Daniel V T Catenacci, Gustavo Cervantes, Soheil Yala, Erik A Nelson, Essam El-Hashani, Rajani Kanteti, Mohamed El Dinali, Rifat Hasina, Johannes Brägelmann, Tanguy Seiwert, Michele Sanicola, Les Henderson, Tatyana A Grushko, Olufunmilayo Olopade, Theodore Karrison, Yung-Jue Bang, Woo Ho Kim, Maria Tretiakova, Everett Vokes, David A Frank, Hedy L Kindler, Heather Huet, Ravi Salgia (2011)
Cancer biology & therapy 12 (1) p. 9-46

Microtubule remodelling is required for the front-rear polarity switch during contact inhibition of locomotion.
Shereen Kadir, Jonathan W Astin, Lubna Tahtamouni, Paul Martin, Catherine D Nobes (2011)
Journal of cell science 124 (Pt 15) p. 2642-53

Interaction between CD36 and Oxidized LDL Modulates Macrophage Cytoskeletal Functions: A Mechanism of Macrophage Trapping
Young Mi Park (2010)

Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies
Monika Zelman-Femiak, Kun Wang, Kira V. Gromova, Petra Knaus, Gregory S. Harms (2010)
BioTechniques 49 (2) p. 574–579

In situ assembly of macromolecular complexes triggered by light.
Christian Grunwald, Katrin Schulze, Annett Reichel, Victor U Weiss, Dieter Blaas, Jacob Piehler, Karl-Heinz Wiesmüller, Robert Tampé (2010)
Proceedings of the National Academy of Sciences of the United States of America 107 (14) p. 6146-51

STIM1-directed reorganization of microtubules in activated mast cells.
Zuzana Hájková, Viktor Bugajev, Eduarda Dráberová, Stanislav Vinopal, Lubica Dráberová, Jirí Janácek, Petr Dráber, Pavel Dráber (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (2) p. 913-23

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