Super-Resolution
It is less than 20 years since super-resolution arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements in the sub-100 nm range to fluorescence images of sub-cellular structures, allowing researchers to see details only previously possible with electron microscopy.
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Tutorial
21. May 2012 Institute: Leica Microsystems, Wetzlar, GermanyAmong the new super-resolution technologies, stimulated emission depletion (STED) is the most versatile concept. Whether the sample is tissue, e.g. muscle striation details, classical cytoskeletons, nuclear proteins, yeast or bacterial details: STED serves for understanding structure and function by… Read article
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Tutorial
14. May 2012 Institute: Leica Microsystems, Wetzlar, GermanyThis tutorial gives on overview of the excitation of an detection light path with a continuous wave (CW) STED microscope. Read article
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Tutorial
02. May 2012 Institute: Leica Microsystems, Wetzlar, GermanyThis tutorial focusses on the principles of STED super-resolution microscopy. The underlying photo physical processes are explained - e.g. with the help of a Jablonski diagram - and the integration into a confocal laser scanning microscope. Read article
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Article
02. Apr 2012 Institutes: Institut Pasteur, Unit of Dynamic Neuronal Imaging, Department of Neuroscience, Paris, France, IIT - Italian Institute of Technology, Nanophysics, Genoa, ItalyAmong the major steps in the development of Stimulated Emission Depletion (STED) microscopy, the demonstration of the use continuous wave lasers (CW-STED) was certainly contributing the most to a wide dissemination of the method due to the affordability and elegant simplicity of this implementation.… Read article
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Article
09. Mar 2012 Institutes: University of Pennsylvania, School of Medicine, Philadelphia, PA, USA, Children’s Hospital of Philadelphia, Research Institute, Philadelphia, PA, USANatural killer (NK) cells are innate immune effectors that recognize and kill virally infected and tumorigenic cells. Central to their effector function is the formation of an immunological synapse with a target cell followed by directed secretion of cytolytic granules. Read article
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Abstract
05. Mar 2012 Institutes: Max Planck Institute for Experimental Medicine, Göttingen, Germany, Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, GermanyTo explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has made a significant step closer to this goal. Using the STED microscopy developed by Hell, the… Read article
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Article
07. Feb 2012 Institutes: University of California, Molecular and Cell Biology, Berkeley, CA, USA, Harvard Medical School, Department of Cell Biology, Boston, MA, USA, American Society for Cell Biology, Bethesda, MD, USAThe need for data validation and accessibility has never been greater than it is today. We are inundated with information from a multitude of resources, but how can we easily evaluate the accuracy of that data? In the past, the peer review process provided this and was often run by publishers. Read article
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Tutorial
13. Jan 2012 Institute: Leica Microsystems, Wetzlar, GermanyThis video tutorial presents a reliable way to produce samples for super-resolution GSD imaging with a special focus on the mounting step. Stable and flat mounting of the coverslip increases the performance of the overall system, leading to an improved resolution of the GSD image. Read article
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Tutorial
08. Dec 2011Here we present an collection of videos telling the story of the invention of nanometer-scale microscopy by Stefan Hell and explaining the basic principle of STED microscopy. Read article
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Article
01. Dec 2011 Institutes: Leica Microsystems, Wetzlar, Germany, Rockland Immunochemicals Inc., Philadelphia, Pennsylvania, USAStimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction encountered with standard confocal laser scanning microscopes and conventional far-field optical… Read article
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Tutorial
31. Oct 2011 Institute: Leica Microsystems, Wetzlar, GermanyThe localization microscopy technique GSDIM is a proven technology to achieve super-resolution images with a resolution of up to 20 nm. In the following tutorial we will describe the basic principles and features of GSDIM. Read article
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Tutorial
28. Oct 2011 Institute: Leica Microsystems, Wetzlar, GermanyGround state depletion microscopy followed by individual molecule return (GSDIM) is a super-resolution technique based on single molecule localization (Localization Microscopy). To localize single molecules and create a high resolution image the ensemble of overlapping fluorophores (in a… Read article
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Step by Step Guide to the Molecular Basics of GSDIM Microscopy
How Widefield Super-Resolution GSDIM Images are Created
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www.mpibpc.mpg.de/groups/hell/
Department of NanoBiopohotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Director. Prof. Stefan Hell
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Collection of Journals and Proceedings in Optics and Photonics
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International, peer-reviewed, open-access, online publication
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International Journal for microscopists
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