YouTube video by Leica Microsystems, June, 2014
Rainer Pepperkok is Head of the Advanced Light Microscopy Core Facility and Senior Scientist at the European Molecular Biology Labortory (EMBL) in Heidelberg, Germany. In the course of his studies he is interested in membrane traffic of the early secretory pathway in mammalian cells which he is trying to analyze with the help of most modern light microcopy techniques. Super-resolution microscopy gives him great hope to get a more detailed view of his field of interest. These in particular are transport vesicles which cannot be resolved by conventional light microscopy because of their diameter of about only 50 ... 60 nm. Being inspired by the first super-resolution image he saw, he looks forward to solve now problems which couldn't for a hundreds of years. Thereby he is sure that both, GSDIM and STED microscopy will play a crucial role in the observation of living and fixed cells. Especially the latest innovations in super resolution microscopy, which enable the user to image their specimen in all three spatial dimensions, he sees as a significant improvement to watch entire organelles as an example. Although Rainer Pepperkok is very pleased by the invention of super-resolution techniques he does not ignore the more complex constraints to be fulfilled. In this respect he puts emphasis on a careful sample preparation and preservation as well as a stringently controlled imaging surrounding to prevent from vibrations. Finally asking him for a comment on his collaboration with Leica Microsystems, he looks back to more than twenty years of fruitful intellectual and technical exchange which he hopes to carry on in the future.