Video Talk by Roger Tsien: Fluorescent Protein Indicators

August 16, 2016

In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides.

The talk also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity or wavelength change, as well as reporters using resonance energy transfer (FRET).

YouTube video by iBioEducation

About iBiology

iBiology (, formerly and was developed to bring the best biology to people throughout the world for free. Started in 2006 by University of California, San Francisco and Howard Hughes Medical Institute investigator, Professor Ron Vale, iBiology has grown to include over 300 seminars and short talks by the world’s leading scientists. Our collection includes talks by many Nobel Laureates and members of the United States National Academy of Sciences. In 2013, we released our first full-length course in Light Microscopy and expanded the educational resources we offer. iBiology is funded by the National Science Foundation and the National Institute of General Medical Sciences. iBiology is supported by the American Society for Cell Biology, the University of California, San Francisco, and the Howard Hughes Medical Institute, each of which has provided essential resources, funding, and in-kind services to iBiology since the project was started.