Wall Cress Pod Protocol - Critical Point Drying of Arabidopsis thaliana for SEM

Application Note for Leica EM CPD300 - Life Science Research

October 25, 2016

Introduction
Species: Wall Cress (Arabidopsis thaliana)
Critical point drying of wall cress pod using the Leica EM CPD300 with subsequent gold coating and SEM analysis.

Procedure
Sample Holder:
Samples were inserted into the 22 mm cover slip holder.

Fixation and Dehydration

3% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.0overnight
0.1M Sodium Phosphate Buffer, pH 7.03x10 min.
Ethanol series: 30%, 50%, 70%, 80%, 90%, 95%, 100%2x10 min.

Leica EM CPD300 auto program

Coating
Gold: 15-20 nm

Fig.1: Arabidopsis Pod

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