In addition to a review of the principles of the method and system architecture, we will expand on specific guidelines for sample preparation, suitable fluorophores and labeling strategies. We will also highlight selected application examples, including super resolution imaging of intracellular substructures such as cytoskeleton elements and associated proteins, nuclear pore complexes and cellular compartments. In particular for STED imaging we will consider applications requiring multi-colour and live imaging.
Dr. Marko Lampe
Dr. Marko Lampe is an Applications Manager at Leica Microsystems, specializing in super-resolution techniques and sample preparation. Before joining the company, Marko worked in research science, where he gained experience in many forms of microscopy and cell biology, concentrating on super-resolution and single molecule imaging.
Dr. Wernher Fouquet
Dr. Wernher Fouquet graduated in Biomedicine from the University of Würzburg, Germany in 2008 with a thesis on structural architecture of the chemical synapse. He worked for the NeuroCure Neurological Disorder Cluster of Excellence at the Charité Hospital in Berlin, Germany. In December 2010, he became an in-house researcher for Leica Microsystems.