Webinar: Correlative Light Electron Microscopy: 1 + 1 = 3

October 06, 2013

Imaging is one of the key technologies for Biomedical research. In many cases it is useful to obtain data from different imaging modalities. Especially combining 2 techniques in one single experiment, so-called Correlative Microscopy, can and should give a better answer than each technique alone. 1 + 1 = 3. Correlative Light Electron Microscopy (CLEM) can be defined as the "Observation and analysis of one and the same event by both a light and electron microscopy technique". There are many approaches to CLEM depending on the question to be answered. In some cases it may be to find specific cells, e.g. transfected or diseased in a large population. Our main interest is in intracellular trafficking. In our approach an event is therefore first imaged live in the light microscope to acquire the history of an event. The sample is then fixed and analysed in the electron microscope. The same event can now be analysed at higher resolution and in addition EM provides us with the surrounding ultrastructure as a reference space. A CLEM experiment can in general be divided in 3 steps: probes, processing, and analysis. In the webinar I will highlight aspects of each these steps and focus on the Leica EM PACT2 + RTS and its use in the CLEM workflow.

Register now to view the webinar

Participate in the free webinar "Correlative Light Electron Microscopy: 1 + 1 = 3"

Speaker

 

Paul Verkade

Chair of the Cryo Microscopy Group, Bristol University

Dr. Paul Verkade’s research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc time at the EMBL, Heidelberg, Germany in the group of Kai Simons and setting up a new EM lab at the Max Planck Institute for Molecular Cell Biology in Dresden, Germany he moved to the University of Bristol, UK in 2006. Here he set up a new EM unit as part of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre.

To support his transport studies, part of his research is to develop techniques and tools for the use of Correlative Light Electron Microscopy (CLEM). Amongst other things he has developed the Rapid Transfer System for the EM PACT2 high-pressure freezer together with Leica Microsystems. This allows for the combination of time-resolved CLEM with optimal preservation of ultrastructure for EM.

Dr. Verkade is currently chair of the Cryo Microscopy Group, affiliated to the Royal Microscopy Society (RMS) and Honorary Secretary of the EM committee of the RMS. He has organised and taught on a number of courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. He is also the principle organiser of the EMBO practical course on CLEM.

Comments