Webinar: Dissecting Protein Dynamics in Living Cells by FRAP

October 29, 2015

This webinar you will learn about how to use Fluorescence Recovery After Photo-bleaching (FRAP) microscopy to study protein dynamics.

Dr. Marco Fritzsche will take a look at the advantages of FRAP microscopy to study the binding dynamics of proteins associated to the cytoskeleton and the plasma membrane of living cells. He will discuss sample preparation, calibration, and application of an optimal FRAP protocol and give an overview about the type of protein dynamics that can be measured by FRAP.

The broadly applicable method based on FRAP he will present answer the following questions:

  • How many reaction processes participate in the turnover of any given protein of interest?
  • How their apparent association and dissociation rates are characterized?
  • What is their relative importance in the turnover of the overall protein population?
  • How to identify the protein domains that mediate each of the identified turnover processes?

Jennifer Horner will give a brief introduction to the Leica DMi8. This open and freely configurable inverted research microscope for live cell imaging is equipped with an additional incident illumination port. This Infinity Port facilitates the integration of additional light sources and laser systems for advanced applications, including FRAP.

Register to view the webinar on demand:

Dissecting protein dynamics in living cells by FRAP

Speakers:

 
Marco Fritzsche

Senior Researcher
University of Oxford


Jennifer Horner

Global Product Manager
Leica Microsystems, Inc.

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