Webinar: From Live Cell Imaging to Super-Resolution

February 07, 2013

The beauty of cells, magnified and resolved by light microscopy, has been fascinating investigators since the 19th century. Today, functional research in living cells is often a prerequisite for biological studies. And keeping the cells close to the necessary conditions whilst under microscopical observation is key.

In this webinar, Dr Christoph Bauer will share his experiences of live cell imaging and talk about how the microscopy challenges, such as optical aberrations at 37 °C to or focus drift in long-term experiments, can be addressed. This webinar will also talk about the challenge of overcoming the diffraction limit. Several approaches to overcome this limitation have been developed and super-resolution microscopy has proven an extremely valuable tool.

The gated STED technology realized on the confocal platforms Leica TCS SP5 and SP8 drives live cell super-resolution to the next level. Details smaller than 50 nm can be revealed and super-resolved dynamic studies performed with common fluorescence proteins. Resolution can be further increased by with localization microscopy techniques, such as dSTORM or GSDIM, as implemented on the Leica SR GSD. This technology can be used to achieve an optical resolution up to 20 nm on fixed specimens – such resolution has been possible only through electron microscopy in the past.

Nowadays, light microscopy needs to handle living cells over multiple days or weeks all the way through to super-resolution images for the visualization of super-fine structures. This webinar will provide you with practical information about live cell imaging and super-resolution conditions and highlight the optimal tools available.

Register now and view the webinar on demand:

From Live Cell Imaging to Super-Resolution

 

 

Speakers

 

Christoph Bauer, Ph.D.

Head of the Bioimaging Platform at the University of Geneva

Covering fluorescence, super-resolution, high content screening and electron microscopy, the facility provides comprehensive microscopy support for a wide range of scientific projects. With a wealth of experience in cell biology, Dr. Bauer also spent time in the United States as an Assistant Professor at the University of Chicago. His past research projects include working on the Alzheimer precursor protein, protein synthesis and the cytoskeleton and parasite host-cell invasion.

 

Christian May, Ph.D.

He joined Leica Microsystem in Wetzlar, Germany, in January 2001 as Product Manager for Laser Microdissection. He changed his focus to TIRF and advanced fluorescence, followed by responsibility for widefield super-resolution microscopy with the Leica SR GSD. He studied Chemistry at the University of Marburg. During his Ph.D. thesis he changed his focus to Biochemistry with main interest in mobilization of lipid bodies in plants. During his postdoc time at the Medical Research department in Marburg, Christian worked mainly on antigen presentation with a main interest in TAP1/2. Here he used one of the first TIRF systems build by Till Photonics/Olympus.

 

Jochen Sieber

Product Manager Super-resolution Technologies, Leica Microsystems

He studied biochemistry in Leipzig, Germany and in Glasgow, UK. He acquired his doctor’s degree at the neurobiology department of the Max-Planck-Institute for biophysical chemistry in Göttingen. There, he was exemplarily characterizing the Syntaxin 1-Cluster to acquire further knowledge about the architecture and dynamics of the plasma membrane. The co-operation with Prof. Stefan Hell provided Sieber with the opportunity to determine size, number and density of this nanostructure via STED-Microscopy. In 2007, he became Application Developer Focus Super-resolution before becoming Product Manager Super-resolution in 2010.

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