Decipher the cellular and molecular interactions that drives disease are a prerequisite for discovering new drug targets. The mixture of various cell types within the tissue can affect the outcome of molecular studies.
Laser Microdissection (LMD) is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The dissectate is then available for further molecular biological methods such as PCR, real-time PCR, proteomics and other analytical techniques. A variety of downstream applications exist: genomics (DNA), transcriptomics (mRNA, miRNA), proteomics, metabolomics, and even next generation sequencing (NGS). In addition LMD is also a perfect tool for cloning, re-cultivation and manipulation of live cell culture (LCC).
In this webinar, learn about the advantages of using Leica Microsystems LMD techniques for precise, contamination-free isolation of specific cell types in various applications. Using pancreatic cancer tissue sections from patients and ortothopic xenografts as examples, this webinar will provide an overview of the scientific and practical considerations for obtaining highly pure material for further molecular analysis in the field of pharmacological studies to overcome key mechanisms of resistance in pancreatic cancer.