Wrinkled Giant Hyssop Leaf Protocol

Application Note for Leica EM CPD300 - Life Science Research

August 11, 2016

Introduction
Species: Wrinkled Giant Hyssop (Agastache rugosa)
Critical point drying of wrinkled giant hyssop leaf with subsequent gold coating and SEM analysis

Procedure
Sample Holder: Samples were inserted into the 22 mm cover slip holder

Fixation and Dehydration

2.5% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.214 h
0.1M Sodium Phosphate Buffer, pH 7.23x10 min.
Acetone series: 30%, 50%, 70%, 80%, 90%, 95%, 100%2x10 min. 

Leica EM CPD300 auto program


Coating
Gold: 15-20 nm

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Fig.1: The leaf of Wrinkled Giant Hyssop
Fig.2: The leaf of Wrinkled Giant Hyssop

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