High Pressure Freezing    
Applications with the Leica EM PACT2 + EM RTS
High Pressure Freezer with Rapid Transfer System
   

Leica EM PACT2 is the first step in the preparation of samples for many subsequent techniques, allowing high quality freezing up to 200 µm into the specimen. The Rapid Transfer System, EM RTS, allows an ease of sample preparation never before experienced in the field of high pressure freezing, minimizing user actions.
   
Leica EM PACT2 + EM RTS - Mouse embryonic fibroblast
 
Mouse embryonic fibroblast grown on sapphire disc. All prominent features of the cell are well frozen and clearly visualized. Cloc ...  
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Leica EM PACT2 + EM RTS - MDCK Cell
 
Semi-polarized MDCK cell frozen with the live cell imaging carrier. The sample was freeze substituted to Epon. Zoom area shows jun ...  
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Leica EM PACT2 + EM RTS - Yeast
 
Dividing Yeast (S.cerevisiae) freeze-substituted to Epon. Zoom shows ribosomes on the nuclear membrane and microtubules in the nuc ...  
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Leica EM PACT2 + EM RTS - C.elegans head in cross-section
 
Structural details of the C.elegans head in cross-sectionT.Müller-Reichert (MPI-CBG, Dresden, Germany) and Kent Mc Donald ...  
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Leica EM PACT2 + EM RTS - PtK2 cell
 
Ultrastructural preservation of different organelles of the PtK2 cell. Clockwise from top right:Mitochondria,Golgi are ...  
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