FAQ Possible Problems on Cryosectioning
Frost/Condensation Problems
   
01.   What might be the reason of frost on chamber walls and the microtome (while the cryochamber lid is in place)?
   
  1. Automated defrost cycle not activated.
  2. Poor air circulation around cryostat.
  3. Sliding window seal is defective. Replace seal.
02.   What might be the reason for frost on chamber walls and the microtome?
   
  1. Locating the cryostat in an area where it is exposed to air currents (open windows or doors, overhead air vents, etc). A more suitable area should be found.
  2. Leaving the sliding window of the cryostat open for an extended pe read more ...
03.   How to avoid condensation on the anti-roll plate and knife during cleaning?
    Ensure that all tools, brushes, forceps, wipes etc. are pre-cooled in the chamber before use. Store all tools on storage shelf in the cryochamber.
04.   What to do if the sliding window collects condensation?
   
  1. Room temperature and humidity might be too high, check the installation requirements, and adjust as required.
  2. Verify that the heater in the sliding window is operating.