Overview
The intelligent automated system for demanding research applications. With motorized z-focus, coded 7x nosepiece, fully automated DIC, and 5x or 8x fluo axis.
As digital microscope platform the upright Leica DM5000 B allocates all transmitted light contrasting methods including the worlds first fully automated differential interference contrast (DIC). With absolute reproducible shearing and bias values. The Z drive is motorized, providing the conveniences of automatic parfocality adjustment, saved focus levels, and automated positioning of various z levels. Dedicated software solutions together with the motorized fluo axis make the Leica DM5000 B a powerful research microscope for applications like immunofluorescence with deconvolution option, FISH, and live cell imaging.
Key Features
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The Leica DM5500 B offers a motorized z-focus drive in addition to all the functions of the DM5000 B
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Automated parfocal compensation and saving of focus positions
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Ideal for time-lapse and z-stack experiments
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Perfect system for FISH applications – the Leica DM5500 B with Leica CW4000 software creates an efficient cytogenetics workstation solution
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Automated transmitted light axis, including Leica’s unique, fully automated DIC
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Motorized fluorescence axis with 5 or 8-position filter cube turret
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Leica’s unique fluorescence intensity manager (FIM) for fast, accurate, and reproducible adjustment of fluorescence lighting
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Motorized Excitation Manager and fast internal filter wheel (IFW) for multiple fluorescences
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New: manual, absolute coded 7x objective turret for even faster response times
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New: convenient Leica SmartTouch touchscreen for controlling the automated modules
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New: optional, external Leica STP6000 SmartTouch Panel control for intuitive, convenient remote control of the microscope
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As for all automated Leica DigitalMicroscopes the transmitted light axis features automated aperture and field diaphragms as well as Leica’s CCIC*. The Constant Colour Intensity Control automatically maintains the colour temperature at the same level even when the lamp voltage is changed.
The worlds first fully automated Differential Interference ContrastNo circumstantial playing with sliders or lose prisms, manual polarizers and analysers. Always the correct combination of prisms for best image quality – fast and reliable. Even for untrained microscopists the results are breathtaking. Users can select different shearing according to the specimen. And of course- the individual bias is stored for each objective and prism combination and can be restored whenever needed.
Motorized z-focusThe z drive of Leica DM5500 B is motorized, providing the conveniences of an automatic parfocality adjustment. You benefit from saved focus levels for each objective, and automated repositioning of various z levels. You are using petri dishes for live cell imaging? On demand the stage is lowered automatically when you switch to another objective.
Shutters** for transmitted light and fluorescence axisAll DigitalMicroscopes starting from Leica DM4000 B up to the Leica DM6000 B are equipped with two independently operated shutters. Ideal for time lapse recordings to protect your specimen.
* patents: DE101 32 360; US 7,027,238
** patents: DE103 09 340; US 6,980,376
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The aim of our intelligent automation is to make it as easy as possible for you. Core of the automation are “electronic managers” for various purposes. Common for all these managers is their flexibility and the perfect adaptation to the users preferences. Tailor your system to perfectly suit your demands.
Contrast ManagerBenefit from the speed and the accuracy of the Contrast Manager. It supports all transmitted light and fluorescence contrasting methods and allows to switch from one contrast method to another just by one button click. Depending on the selected objective all necessary adjustments are done automatically. In case of Differential Interference Contrast (DIC) the values of shearing and bias are stored together with each objective to offer reproducible results.
Illumination ManagerOur DigitalMicroscopes automatically recognize the contrasting technique and objective that is currently in use. And of course it memorizes automatically the last used settings for this combination and recalls them. There's no need to adjust circumstantially any more diaphragms or the light intensity values- just another example how Leica’s microscope managers ease your work.
Variable Function KeysYou can assign any function you like to the 10 freely programmable function keys. Due to their convenient position, frequently used functions are always within easy reach.
Integrated Leica touchscreenIntegrated in the base of the microscope, ergonomically placed is the unique Leica SmartTouch touchscreen. All motorized functions can be easily controlled and monitored.
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The Leica DM5500 B offers a fully motorized 5 fold or 8 fold fluorescence axis. The brilliant fluorescence images are based on Leica’s light trap to reduce background, and zero pixel shift fluorescence cubes. Of course all components are apochromatic corrected. But nowadays you can expect more from the leading supplier of fluorescence systems. Convince yourself.
Fast Internal Filter wheels
In addition to the well known FIM* (Fluorescence Intensity Manager) of the Leica DM4000 B the Leica DM5500 B can be additional equipped with an fast internal filter wheel. It is used in combination with a triple or double filter cube. The filter wheel combines high-speed with vibration free movement for ultra-fast change of excitation light.
Motorized and coded Excitation Manager**For triple or double stained immunofluorescence samples the intensities of different fluorochromes are always an issue for researchers. With the “Excitation Manager” the intensities can be carefully balanced by means of optical reduction or enhancement of particular excitation light. furthermore the values can be stored and restored for reproducible results.
Ultrafast external filter wheelsA wide variety of Leicas ultrafast external filterwheels support sophisticated fluorescence application up to calcium-ratio imaging and FRET. Both applications require speed and superior imaging performance. Fully integrated in Leica AF6000 fluorescence software as a seamless solution from one hand.
New Fluorescence lampThe new external light source Leica EL6000 is a plug and play fluorescence lamp with a life span of approximately 2000h. No time consuming adjustments needed anymore. In addition the Leica EL6000 is equipped with a ultrafast shutter-of course fully integrated in the microscope control either direct or via software.
Documentation devicesFor the Leica DM5500 B a broad variety of documentation tubes is available. With one or two camera exits. Optional also as motorized variant allowing the fast switching form a high speed black and white camera to a high resolution colour camera. Ideal prerequisite for switching between immunofluorescence and life cell imaging.
* patent pending DE 102 57 521
** patens DE 198 58 206 ; US 6,747,280
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Harmonized integration of hardware and software is the “sine qua non” for demanding applications. Leica as provider of microscopes, cameras and software is able to meet these demands from one source. Our philosophy is to offer a wide range of application solutions starting from simple documentation up to the most advanced fluorescence analysis workstation.
LAS (Leica Application Suite)For multi purpose use the free of charge LAS core software for microscope and camera control can be upgraded with various modules. Starting from basic documentation up to leading edge multi channel fluorescence imaging and quantification tools. Your application is changing? You have new targets? No problem – the LAS software platform is upgradeable at all times- now and in the future.
Advanced Fluorescence workstation Leica AF6000The new Leica AF6000 software has been designed to meet even the highest demanding fluorescence applications. Ultrafast, multidimensional fluorescence imaging, deconvolution, 3D visualization and quantification are just a few examples of the wide spectrum of application solutions offered by AF6000 software. The simplicity of the user interface will convince you.
The right camera for all applicationsThe Leica DFC range of digital cameras offers the right camera for every requirement. From general to specific, our cameras are ideal: colour or grey-level pictures of fluorescence specimens or pathological materials. The camera chips feature up to 12 mega pixel image resolution.
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