Grand Opening

Join the Opening Ceremonies at the NEW Leica Center of Excellence at Yale West Campus

On October 20 and 21, 2014, Yale University West Campus will open the doors to a new microscopy Center of Excellence (CoE) made possible through a collaboration with Leica Microsystems. Housed in a newly created core facility, the Leica Center of Excellence at Yale West Campus will provide scientists with access to cutting-edge imaging tools to resolve sub-cellular structures and forward scientific discoveries. This relationship will afford researchers access to expert technical and applications support, along with instrumentation which Leica Microsystems will continuously update with the latest technology. In addition, Yale and Leica Microsystems will collaborate in the development of novel imaging technologies.

Limited Spaces Available for Opening Sessions and Microscopy Workshops

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Event Schedule

Christopher Incarvito, Ph.D., Director of Research Operations & Technology at Yale University’s West Campus, invites scientists to take part in a two-day Grand Opening celebration announcing the development of a first-of-its-kind microscopy Center of Excellence in North America. These two days will feature scientific, applications and technology talks, round table discussions and a keynote address from Joerg Bewersdorf Ph.D., Associate Professor of Cell Biology and of Biomedical Engineering at the Yale School of Medicine.

Leica Center of Excellence at Yale West Campus
Grand Opening Celebration

October 20-21, 2014

Schedule

Monday, October 20, 2014 – “Celebrate the Vision Being Realized”

Location: Yale West Campus Conference Center | 800 West Campus Drive | Grace Murray Hopper Auditorium

 

11:30 AM – 12:30 PMComplimentary Lunch, Courtesy of Leica Microsystems
12:30 – 12:50 PMWelcome and Introduction
Chris Incarvito, Ph.D., Director of Research Operations and Technology, Yale University
Julian Burke, Ph.D., Chief Scientific Officer, Leica Microsystems
12:55 – 1:15 PMLeica Microsystems - A Company of Innovation
Anthony Wieczorek - Director of Sales, Leica Microsystems
1:15 – 1:25 PMCollaborative Discovery at the Leica Center of Excellence at Yale West Campus
Douglas Giszczynski, Area Sales Manager, Leica Microsystems
1:25 – 1:45 PM West Campus Imaging Center, Goals and Development Strategy
Vladimir Polejaev, Ph.D., Imaging Director, Yale University West Campus
1:45 – 2:15 PMBreak
Rapid Fire Science and Technology Presentations
2:15 – 2:30 PMCell Size Homeostasis in Bacteria
Manuel Campos, Ph.D., Microbial Sciences Ins., Yale University
2:30 – 2:45 PMModular Concept of Leica DMi8
Bernd Saegmueller, Ph.D., Director, Confocal Marketing, Leica Microsystems
2:45 – 3:00 PMSingle Vesicle Fusion Assay with Membrane Tension Control
Joerg Nikolaus, Ph.D., Nanobiology Ins., Yale University
3:00 - 3:15 PMUsing the Infinity Access Port on the Leica DMi8
Marcus Dyba, Ph.D., Senior Projektleiter Technologie, Leica Microsystems
3:15 - 3:30 PMA New Oxygen Scavenger for Super-Resolution Imaging
Tobias Hartwich, Ph.D., Nanobiology Ins., Yale University
3:30 - 3:45 PMLeica Confocal Imaging Phylosophy: AOBS, WLL, SP
Haridas Pudavar, Ph.D., Sr. Product Specialist, Leica Microsystems
3:45 - 4:00 PMLive Cell STED Microscopy
Edward Allgeyer, Ph.D., Cell Biology Dept., Yale University
4:00 - 4:15 PMAdvantages of HyD Detectors
Marcus Dyba, Ph.D., Senior Projektleiter Technologie, Leica Microsystems
4:15 - 4:30 PMWalk to the Center of Excellence
4:30 - 5:30 PMRibbon Cutting Ceremony - Wine and Cheese Reception
Chris Incarvito, Ph.D., Director of Research Operations and Technology, Yale University
Julian Burke, Ph.D., Chief Scientific Officer, Leica Microsystems

Tuesday, October 21, 2014 – “Exploring How We Can Best to Work Together”

Location: Yale West Campus Conference Center | 800 West Campus Drive | Grace Murray Hopper Auditorium

 

9:00 – 9:45 AMCoffee and meet and greet
9:45 – 10:00 AMAuditorium Opens - Seating Available
10:00 – 10:30 AMMicroscopy Methods to Study Membrane Fusion with Single Event Resolution
Erdem Karatekin Ph.D., Nanobiology Ins., Yale University
10:30 – 11:00 AMSuper-Resolution - Deeper Insights into the Technology
Marcus Dyba, Ph.D., Senior Projektleiter Technologie, Leica Microsystems
11:00 – 11:30 AMEveryday Super-Resolution - Towards Reliable and Quantitative Imaging in Challenging Samples
David Baddeley, Ph.D., Nanobiology Ins., Yale University
11:30 AM – 12:00 PMLight Sheet Imaging
Bernd Saegmueller, Ph.D., Director, Confocal Marketing, Leica Microsystems
12:00 – 1:30 PMComplimentary Lunch, Courtesy of Leica Microsystems
1:30 – 2:00 PMCross-talk between the Endoplasmic Reticulum and the Plasma Membrane Mediated by Extended Synaptotagmins
Yasunori Saheki, M.D., Ph.D., Cell Biology Dept., Howard Hughes Medical Institute
2:00 – 2:45 PMKeynote Address: Live-Cell Single Molecule Switching Nanoscopy
Joerg Bewersdorf, Ph.D., Cell Biology Dept., Yale University
2:45 - 3:00 PMClosing Remarks
Chris Incarvito, Ph.D., Director of Research Operations and Technology, Yale University
Julian Burke, Ph.D., Chief Scientific Officer, Leica Microsystems

Register Now

 

 

Super-Resolution Workshops

Free Super-Resolution Microscopy Hands-on Workshops at Yale West Campus

To kick off the Leica Center of Excellence at Yale West Campus Grand Opening celebrations, Leica Microsystems presented three seminars Oct. 7-8, 2014 covering different solutions for multi-color, 3D imaging beyond the diffraction limit.

Hands-on workshops, scheduled in the days following the Center of Excellence Grand Opening celebrations, will be offered. To request time on the Leica TCS SP8 STED 3X or the Leica SR GSD 3D system, please choose from the available time slots on the calendars below and enter that time in the Sign-up Form below. Once you’ve made your request, you will receive a confirmation email within 24 hours.

Super-Resolution Hands-On Workshops (Following Center of Excellence Grand Opening)

  • October 22-24, 29, 2014
    GSTED/GSD
  • October 27-28, 2014
    GSD
  • October 30-31, 2014
    GSTED

Register Now

The Leica SR GSD 3D
Widefield Super-Resolution

The Leica SR GSD 3D super-resolution microscope based on the GSDIM (Ground State Depletion with Individual Molecule return) method which is also referred to as dSTORM (Direct STochastical Optical Reconstruction Microscopy). Leica GSD technology offers 2D and 3D super-resolution imaging – with lateral resolution (XY) of 20nm and axial resolution (Z) of 50nm - with biological samples.  The Leica SR GSD 3D is based on fully automated Leica TIRF microscope and features a 160X objective lens, state of the art stability mechanisms, and 3 powerful lasers for GSDIM operation: 488nm, 532nm, and 647nm. The most appropriate samples for the GSD include fixed, immunofluorescently labeled cells and tissues that are mounted to #1.5 glass coverslips.  Samples that are routinely imaged with TIRF microscopy will be excellent candidates.  Most conventional fluorochromes are well suited for the GSD, especially Alexa647, Alexa488, and Alexa532.  Samples must be brought as unmounted coverslips (mounting to slides will occur just prior to imaging).

Sample Preparation for Ground State Depletion Microscopy

The Leica TCS SP8 STED 3X
Confocal Super-Resolution

The Leica TCS SP8 STED 3X is a confocal super-resolution microscope based on the Stimulated Emission Depletion (STED) method that provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. STED 3X facilitates multi-color imaging by providing multiple STED laser wavelengths of 592 nm, 660 nm, and 775 nm. The 3D module for STED 3X generates two different STED patterns at the sample plane to improve resolution in all dimensions.  Lateral resolution (XY) is improved down to 50 nm by the vortex filter while the Z-Donut improves axial resolution (Z) down to 130 nm.  Gated STED gives the user the option to further improve image resolution or to lower laser power at the sample.  STED technology is ideal for samples that already image well on a point scanning confocal but have structures smaller than the 200 nm lateral and 500nm axial resolution.  Most conventional  fluorochromes are well suited for STED, especially eGFP, eYFP, CY3, Alexa 568, Alexa 594 and mStrawberry. Samples should be prepared and mounted for an inverted microscope and not include DAPI staining.

Sample Preparation for Stimulated Emission Depletion Microscopy

About the CoE

Leica Center of Excellence at Yale West Campus

A first-of-its-kind microscopy Center of Excellence in North America, offering the latest advances in imaging technology

Yale West Campus
850 West Campus Drive
Integrated Science and Technology Center
Room 006

Yale University and Leica Microsystems are pleased to announce the establishment of a new microscopy Center of Excellence in the Yale West Campus. Yale University's West Campus has extensive laboratory space where experts from diverse disciplines work side by side using innovative technologies to address important issues in science, art conservation, health, energy, and the environment.

The six research institutes bring together experts from Yale’s top-ranking programs in the physical, biological, engineering, and medical disciplines, as well as other departments. Yale University's West Campus layout is designed to promote interdisciplinary collaboration and improved scientific discovery efficiency in the fields of chemical biology, cancer biology, nanobiology, systems biology, microbial diversity, and energy sciences.

Through this collaboration, Leica Microsystems gains access to world-class scientists and the latest developments in research applications. Yale and Leica Microsystems will work together to test prototype products and share ideas on how to improve imaging workflows.

“We are extremely excited to embark on this journey of discovery with Yale University as our first such collaboration in the USA,” said Doug Reed, General Manager of Leica Microsystems North America. “This cooperation will provide some of the best scientific minds access to imaging tools previously out of reach, plus allow Leica Microsystems ready access to new ideas from outstanding scientific leaders, which will be used to guide development of tomorrow’s innovations.”

The concept for the Center of Excellence is in complete agreement with the Yale West Campus philosophy of collaboration. Leica Microsystems’ historically close cooperation with the scientific community is the key to its own tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements.

Contact Us Today

Contact us to register for an event, reserve instrument time, or ask a question about the CoE.

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Microscopes at the Leica Center of Excellence at Yale West Campus

Leica TCS SP8 X

Leica TCS SP8 X can be used to tune excitation wavelengths to perfectly match fluorophores’ excitation spectra, reduce laser power and increase viability for long live cell experiments.

Leica LMD7000

Contact-free and contamination-free Laser Microdissection (LMD) makes it possible to analyze cells or tissues of interest with precision. It enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material.

Leica DMi8

As your research and lab grows, the Leica DMi8 is able to grow with you. Its unique flexible design allows you to configure and build a microscope that fits your needs and budget today, while offering upgradability for future research needs.

Leica DM IL LED

The Leica DM IL LED for tissue culture experiments features high stability, plenty of space to work with tools, long working distances for large culture flasks, illumination without heat, and the separate electronics unit make work at the microscope easy and convenient.

Leica M125

The modular Leica M125 fully apochromatic corrected stereomicroscope with 12.5:1 zoom allows a wide sample overview and structural detail down to 1.15µm resolution for detailed, high contrast imaging.


Register for Leica Center of Excellence at Yale West Campus workshops, symposium, instrument time or talk to one of our imaging experts using the form below.

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