Standard electron microscopy techniques based on elaborate protocols identify glomerular diseases based on two-dimensional information. The combination of optical clearing and 3D STED Nanoscopy (Stimulated Emission Depletion) with the Leica TCS SP8 STED 3X opens a new avenue for imaging the kidney in unprecedented three-dimensional detail in light microscopy.
Top image: Cleared kidney sample, revealing the podocyte celllayer; magnified podocytes by STED and electron microscopy (EM). Cleared kidney sample courtesy: David Unnersjö-Jess, KTH, Stockholm, Sweden. Scanning EM image of kidney podocytes courtesy: Center for Microscopy and Image Analysis, Univ. of Zurich, Dr. Urs Ziegler. Scale bar is 1 µm.
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Even deeper than 150 µm within the tissue, the ultrastructural features of the specimen come to light powered by the new STED WHITE glycerol objective lens. This is achieved not only at 23°C, but also under live cell imaging conditions at 37°C with a comfortable working distance of 300 µm.
Forget the hassle of physical sectioning. Enjoy spectral freedom and keep up with the growing palette of fluorescent biomarkers.
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Nanoscopy with the TCS SP8 STED system equipped with the HC PL