Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures. Read more about fluorescence microscopy, quantitative fluorescence and super-resolution on Leica Science Lab.
Our experts on solutions for fluorescence applications are happy to help you with their advice.
For routine to live cell research
(Semi)-automated fluorescence stereo microscopes
4.2 MP cooled monochrome fluorescence scmos camera for advanced applications like high-speed live cell imaging, FRAP, and ratio measurement
Increase work efficiency with Leica DM4 B & DM6 B upright digital research microscopes.
Ergonomic optical microscope systems for routine and research applications
Uniquely ergonomic system microscope with LED illumination
1.3 MP monochrome camera for basic fluorescence applications such as documentation of fixed, immunostained samples
Ergonomic System Microscopes for Complex Clinical Applications
Fixed Stage Microscope for Electrophysiology and in vivo Imaging
Fluorescence Microscope System for Advanced Imaging and Analysis
Uniquely Ergonomic System Microscopes With Intelligent Automation
Fluorescence stereomicroscope accessory
Fluorescence microscopes used in research applications are based on a set of optical filters:
The filters are often plugged in together in a filter cube (compound microscopes) or in a flat holder (mainly stereo microscopes).
Whereas the excitation filter selects the wavelengths to excite a particular dye within the specimen, the emission filter serves as a kind of quality control by letting only the wavelengths of interest emitted by the fluorophore pass through. The dichroic mirror's purpose is to reflect light in the excitation band and transmit light in the emission band, enabling the classic epifluorescence incident light illumination.
This fluorescence tutorial explains the optical elements in the light path and the operating mode of fluorescence microscopy taking the example of an inverted microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.
Leica Microsystems’ fluorescence stereo microscopes use the TripleBeam technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror.
Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.
To acquire fluorescence images, it is necessary to use the appropriate