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Fluorescence Microscopy

Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures. Read more about fluorescence microscopy, quantitative fluorescence and super-resolution on Leica Science Lab.

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Our experts on solutions for fluorescence applications are happy to help you with their advice.

Fluorescence Microscopes & Imaging Products 27

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THUNDER Imager Model Organism

THUNDER Imager Model Organism

The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.

THUNDER Imager 3D Live Cell & 3D Cell Culture

THUNDER Imager 3D Live Cell & 3D Cell Culture

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

THUNDER Imager Tissue

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.

Leica DFC9000

Leica DFC9000

4.2 MP cooled monochrome fluorescence scmos camera for advanced applications like high-speed live cell imaging, FRAP, and ratio measurement

LAS X Life Science

LAS X Life Science

Software platform for life science applications

Leica M205 FCA & Leica M205 FA

Leica M205 FCA & Leica M205 FA

(Semi)-automated fluorescence stereo microscopes

Leica DM4 B & DM6 B

Leica DM4 B & DM6 B

Increase work efficiency with Leica DM4 B & DM6 B upright digital research microscopes.

Leica DM2500 & DM2500 LED

Leica DM2500 & DM2500 LED

Ergonomic optical microscope systems for routine and research applications

Leica DM1000 LED

Leica DM1000 LED

Uniquely ergonomic system microscope with LED illumination

Leica DFC3000 G

Leica DFC3000 G

1.3 MP monochrome camera for basic fluorescence applications such as documentation of fixed, immunostained samples

Leica DM IL LED

Leica DM IL LED

Inverted Laboratory Microscope with LED Illumination

Leica DM1000

Leica DM1000

Uniquely Ergonomic System Microscope

Leica DM6 FS

Leica DM6 FS

Fixed Stage Microscope for Electrophysiology and in vivo Imaging

Leica TL4000 BFDF

Leica TL4000 BFDF

Transmitted Light Base For Brightfield and Darkfield Applications Absorbs Shock and Vibrations for High Quality Imaging

Leica FluoCombi III

Leica FluoCombi III

Fluorescence stereomicroscope accessory

Leica LED3000 MCI

Leica LED3000 MCI

LED Multi Contrast Illumination for Routine Stereo Microscopes

LAS X Widefield Systems

LAS X Widefield Systems

Fluorescence Microscope System for Advanced Imaging and Analysis

Leica DM3000 & DM3000 LED

Leica DM3000 & DM3000 LED

Uniquely Ergonomic System Microscopes With Intelligent Automation

Fluorescence Light Microscopes

Fluorescence microscopes used in research applications are based on a set of optical filters:

  • an excitation filter
  • a dichroic beam splitter and
  • an emission filter

The filters are often plugged in together in a filter cube (compound microscopes) or in a flat holder (mainly stereo microscopes).

Whereas the excitation filter selects the wavelengths to excite a particular dye within the specimen, the emission filter serves as a kind of quality control by letting only the wavelengths of interest emitted by the fluorophore pass through. The dichroic mirror's purpose is to reflect light in the excitation band and transmit light in the emission band,  enabling the classic epifluorescence incident light illumination.

This fluorescence tutorial explains the optical elements in the light path and the operating mode of fluorescence microscopy taking the example of an inverted microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.

Fluorescence Stereo Microscopes

Leica Microsystems’ fluorescence stereo microscopes use the TripleBeam technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror.

Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.

Product links

Leica M205 FCA & M205 FA | Leica M165 FC | Leica MZ 10 F | Leica Fluocombi III 

Cameras for fluorescence microscopy

To acquire fluorescence images, it is necessary to use the appropriate CCD or sCMOS fluorescence camera. Leica Microsystems offers dedicated fluorescence cameras for all application needs. Each camera provides the perfect match of sensitivity and image quality to ensure superb results.

Software for fluorescence microscopy

Straightforward setup and control of experiments is becoming more and more important In fluorescence microscopy. The dedicated Leica Application Suite (LAS) X software platform facilitates the setup of even complex experiments. LAS X guides you from the setup to the analysis of the imaging data. From easy multi-channel experiments to complex 3D analysis, LAS X is the perfect software platform.

News

Drosophila melanogaster fruitflies perching on the brim of a bowl of sour dough and waiting for their chance. Image Courtesy: Ursula Gönner, Dolgesheim (Germany).

22. May 2019 Visualize developmental patterns in Drosophila

The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within…

08. May 2019 Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)

The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy)…

18. April 2019 Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…

17. April 2019 Visualize mechanical interactions of Cancer Cells

Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the…

Zebrafish Whole Brain imaging with Leica SP8 spectral confocal laser scanning microscope

09. April 2019 Zebrafish Brain - Whole Organ Imaging at High Resolution

Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the…

11. March 2019 What is a Resonant Scanner?

A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal…

11. March 2019 What is a Spectral Detector (SP Detector)?

The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP…

07. March 2019 Resolved Field Number (RFN)

The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is…

07. March 2019 What is a Hybrid Detector (HyD)?

A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier…

07. March 2019 What is a Field-of-View Scanner?

A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical…