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Laser Microdissection LMD

Laser Microdissection, also known as LMD or LCM (Laser Capture Microdissection), is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The thickness, texture and preparation technique of the original tissue are relatively unimportant. The dissectate is then available for further molecular biological methods such as PCR, real-time PCR, proteomics and other analytical techniques. Laser microdissection is now used in a large number of research fields, e.g. neurology, cancer research, plant analysis, forensics or climate research. The method is meanwhile also applied for manipulation of cell cultures or for microengraving of coverslips.

Our LMD Systems are perfect tools to optimize DNA-Workflows (Genomics), RNA-Workflows (Transcriptomics) and Proteomic-Workflows as they allow to precisely define and collect pure starting material for analysis under visual control.

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Please contact us if you would like to have personal expert advice on our microscopy solutions for laser microdissection.

Leica LMD6500 & LMD7000 Light Microscopes Leica Leica Microsystems

Laser Microdissection Systems Leica LMD6500 & LMD7000

The Leica Laser Microdissection System LMD6500

The LMD6500 is the classic solution for fast, reliable, contact- and contamination-free extraction of desired microscopic samples.

The Leica Laser Microdissection System LMD7000

The Leica LMD7000 has the most powerful and ajustable laser for microdissection of all kinds of tissue and in addition to all options of the LMD6500 the LMD7000 is also able to easily cut hard plant material up to wood of several 100 µm.

Dedicated dry objectives for laser microdissection (5x – 150x)

Example: Laser Microdissection of a mouse aorta.

Frozen section (10 μm) of a mouse aorta (whole vessel) before (A) and after (B) Laser Microdissection stained with cresyl violet on a POL frame slide. Cap inspection allows identification of the dissected section in collector (C). Courtesy of K. Beuerlein, Rudolf-Buchheim-Institut fur Pharmakologie, Justus-Liebig-Universitat Giessen.

News

29. April 2019 Laser Microdissection Publication List

This monthly updated reference list demonstrates the major application fields for laser microdissection in life science research.

27. March 2019 Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection

Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples…

17. October 2018 Webinar: Live Cell Isolation by Laser Microdissection

Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays.…

13. September 2018 Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10–100 Mammalian Cells

Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches…

09. February 2018 Free Webinar On-Demand: How to Conduct Localized Proteomics of Microscopic Regions

In this webinar, you will learn: - How to perform isolated proteomics of microscopic regions of interest - How to use this technique with FFPE…

20. April 2017 Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds

Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like…

07. December 2016 MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer

Application example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the…

06. June 2016 Workflows & Protocols: Plant Laser Microdissection

During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na…

04. May 2016 Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research

Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we…

04. December 2015 Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience

The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is…