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How to gain deep insights into the development of cancer using spectrally tuneable 2-photon microscopy
Understand the complexity of cancer spread by using the right labelling method and imaging techniques.
About the Application Note
Lineage tracing in a confetti animal model combined with multiphoton imaging using the STELLARIS 8 DIVE allows researchers to follow the progression of cancer cells whereas multiphoton imaging is an adequate technique for intravital imaging and deep penetration into tumor tissue.
4Tune, the spectral non-descanned detector in the STELLARIS 8 DIVE, is flexible and enables an optimal setup of detection ranges for each fluoropore, simplifying complex experiments significantly.
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Observing 3D Cell Cultures During Development
3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…Read article
Label-free Differentiation of Microplastics
Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of pixel-accurate lifetime distributions in a sample…Read article
Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…Read article
AI in Microscopy Webinar
We demonstrate residual channel attention networks for restoring and enhancing volumetric time-lapse (4D) fluorescence microscopy data.Read article
20 Years of Leica Laser Microdissection
Phenotype-genotype correlations are key for insight. From Eye to Insight is therefore fitting perfectly to Leica Microsystems and in particular to laser microdissection. Laser Microdissection, also…Read article
Download The Guide to Live Cell Imaging
In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…Read article
Fish and Chips Sheet into the Light
The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general bauplan of the vertebrate brain, however, is…Read article
Enhanced Views in Bypass and AVM Surgeries with Fluorescent Filters
Leica Microsystems is a pioneer in the field of fluorescence microscopy technology, developing innovative fluorescent modules for various medical applications. Our TriFluoro filter concept enables up…Read article
Download EM Workflow Solutions Booklet
This publication is a compilation of appropriate workflows for the most frequently used sample preparation methods, like Correlative Methodologies, Optogenetics & Electro-Physiology, Surface Analysis,…Read article
Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…Read article
Application Booklet for EM TIC 3X
Today, ion beam milling is one of the most widely-used methods for preparing samples for electron microscopy. Download this 76-pages booklet today and learn how to improve your processes.Read article
Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells
Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…Read article
Adding Dimensions to Multiplex Molecular Imaging
Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…Read article
Understanding Motor Sequence Generation Across Spatiotemporal Scales
We have developed a microscopy-based pipeline to characterize a developmentally critical behavior at the pupal stage of development, called the ecdysis sequence. We study brain-wide neuronal activity…Read article
A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…Read article
Benefits of TauContrast to Image Complex Samples
In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or…Read article
Residual Fragment Assessment with the Proveo 8 Intraoperative OCT
In the brief video presented below, Dr. Rachid Tahiri, an ophthalmic surgeon at the Centre d’Ophtalmologie in Granville, France shares his experience in using Proveo 8 intraoperative OCT in cataract…Read article
Cataract Incision Analysis Using EnFocus Intraoperative OCT
Ophthalmic surgery requires high levels of accuracy and precision for optimal surgical maneuvers and tissue manipulation. Surgical microscopes play an essential role in helping ophthalmic surgeons…Read article
Why is Manual Visual Inspection of Medical Devices so Challenging?
This article discusses how manual visual inspection, which is prevalent in the medical device industry, can lead to inconsistent results. It also addresses the challenges quality managers and…Read article
Intraoperative OCT-Assisted Surgical Management of Proliferative Vitreoretinopathy
Proliferative vitreoretinopathy (PVR) is a plague to patients and their surgeons after recent rhegmatogenous retinal detachment (RD). Despite excellent initial surgical outcomes, it is the most common…Read article
Fast, high-quality vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…Read article
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…Read article
Efficiency in angle-based and canal-based glaucoma surgeries
In this interview Dr. Don C. Nguyen explains how the Proveo 8 ophthalmic microscope improves his surgical outcomes during complex cataract surgery, micro-invasive glaucoma surgery, and the early…Read article
Advanced Visualization in ENT Surgery
ENT surgery poses unique challenges. It requires excellent visualization, both in terms of magnification and illumination. As such, choosing the right operating microscope is critical.Read article
Advanced Visualization in Spine Surgery
There have been meaningful advances in spine surgery in recent years. Minimally Invasive Spine Surgery (MISS) techniques provide significant benefits, helping to minimize surgical trauma, improve…Read article
How Augmented Reality is Transforming Vascular Neurosurgery
Augmented Reality is changing surgery, with new information helping to improve the precision and safety of procedures. This is especially true in vascular neurosurgery where Augmented Reality is…Read article
Overcoming Ophthalmologic Surgery Challenges
Ophthalmology surgical procedures involving both the anterior and posterior segment can be particularly challenging. Good visualization is required to operate with precision and confidence. Prof.…Read article
Advanced Visualization in Maxillofacial Plastic and Reconstructive Surgery
Plastic and Reconstructive Surgery is very demanding. Surgical microscopes play an important role, helping to ensure flaps are well vascularized. Dr. Christine Bach is a Plastic and Reconstructive…Read article
Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…Read article
Moving to Routine Use of Intraoperative OCT in Eye Surgery
Dr Barbara Parolini is one of the pioneers in the use of intraoperative OCT in eye surgery. Since 2016, she has gained a lot of clinical experience with different intraoperative OCT systems. In this…Read article
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…Read article
Viral Infections – Studying Influenza-host Interactions in 3D Specimens
Stefan Finke studies virus-host interactions. It turns out that the ways the virus and host interact are different when comparing results from classical laboratory monolayer cell lines and “close to…Read article
GLOW800 Augmented Reality Fluorescence in AVM (Arteriovenous Malformation) treatment
In this case study Prof. Dr. Feres Chaddad talks about the treatment of AVMs. It illustrates how the Augmented Reality Fluorescence GLOW800 can help surgeons during microsurgical resection by…Read article
Finding new Scaffolds for Tissue Engineering
Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…Read article
The Power of Pairing Adaptive Deconvolution with Computational Clearing
Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…Read article
Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics
Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…Read article
Neurovascular Surgery & Augmented Reality Fluorescence
Vascular neurosurgery is highly complex. Surgeons need to be able to rely on robust anatomical information. As such, visualization technologies play an essential role. Prof. Nils Ole Schmidt is a…Read article
How OCT-guided eye surgery can help you to Focus on Perfection
Watch the successful clinical online symposium on-demand. Find out what Dr. Parolini says about the benefits of OCT-guided ophthalmic surgery. She presents clinical cases and shares her experience…Read article
Better Decision Making during Anterior Segment Surgery
Anterior segment surgery ranges from routine cataract surgery to more complex procedures such as Descemet Stripping Automated Endothelial Keratoplasty (DSAEK), Deep Anterior Lamellar Keratoplasty…Read article
Microscopy Enhanced Navigation in Neurosurgery
Neurosurgical procedures are very complex and delicate, especially when they involve craniotomy. Surgeons need to be extremely focused and concentrated throughout. Navigation plays an essential role,…Read article
Topographic Analysis of Firing Pin Impressions on Cartridge Cases
The analysis of fired cartridges for primer cup morphology and flattening and firing pin impression (crater) depth using topographic data is discussed in this article. Topographical analysis of the…Read article
Plant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…Read article
Studying Pulmonary Fibrosis
The results shown in this article demonstrate that fibrotic and non-fibrotic regions of collagen present in mouse lung tissue can be distinguished better with polarized light compared to brightfield.…Read article
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…Read article
Studying Autoimmune Disease
This article discusses how autoimmune diseases, like systemic lupus erythematosus (SLE), can be studied more efficiently using thick, 3D kidney tissue specimens visualized with a THUNDER Imager . SLE…Read article
Intraoperative OCT-Assisted Gene Therapy
Gene augmentation therapy is a method of ocular gene transfer for autosomal recessive or X-linked retinal dystrophies when there is insufficient functional protein manifesting as genetic disease.…Read article
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…Read article
Keeping Particulate Contamination Under Control in Pharmaceutical Products
This article describes how a 2-methods-in-1 solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) can be utilized for identification of particulate contaminants in the…Read article
Writing the NIH Research (R01) grant: Strategies for Success
With highly competitive NIH budgets and ever-increasing restrictions on federal spending, getting noticed and winning an NIH R grant is far more difficult and highly competitive than it has ever been.…Read article
Pursuing private foundation and corporate grant opportunities in Life Science Research
Devon Brewer describes the essentials for pursuing research grants from private foundations and corporations.Read article
How FLIM Microscopy helps to detect microplastic pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…Read article
High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…Read article
Fast Visual and Chemical Analysis of Contamination and Underlying Layers
Visual and chemical analysis of contamination on materials with a 2-methods-in-1 solution leading to an efficient, more complete analysis workflow is described in this report. A 2-in-1 solution,…Read article
Hand-Held OCT Clinical & Research Applications
Hand-held Optical Coherence Tomography (OCT) has revolutionized pediatric ophthalmology and has had a significant impact on ophthalmology in general. The Leica Envisu C2300 hand-held OCT allows to…Read article
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…Read article
Immediate Breast Reconstruction: Benefits of GLOW800 Augmented Reality
Dr. Harold Chatel is a Plastic and Reconstructive Surgeon in a leading hospital in Paris, France. One of his areas of expertise is oncological reconstructive surgery and immediate breast…Read article
Oncological Reconstructive Surgery with the Leica M530 OHX Microscope
Precision is essential in oncological reconstructive surgery, in particular when it relies on free flap techniques. Microsurgical microscopes provide optimal visualization and help streamline the…Read article
Oncological Reconstructive Surgery: Why Use a Microscope
Recent advances in microsurgery are enhancing breast reconstruction for oncology patients, allowing both functional and aesthetic rehabilitation. More and more surgeons are adopting surgical…Read article
Benefits of Microscope & Integrated Vascular Fluorescence Use
Oncological reconstructive surgery requires high precision and attention to details. It can pose a number of challenges: long operating time, flap necrosis and other complications. Leica visualization…Read article
A New World of Confocal Applications with the Next Generation White Light Lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is…Read article
How does an Automated Rating Solution for Steel Inclusions Work?
The rating of non-metallic inclusions (NMIs) to determine steel quality is critical for many industrial applications. For an efficient and cost-effective steel quality evaluation, an automated NMI…Read article
Cornea Webinar Series: DMEK, DSAEK, and DALK cases
Exclusive 2-part webinar series by Dr. Jod S Mehta, distinguished Professor, Duke-NUS, Singapore National Eye Center. Learn DMEK, complicated DSAEK, DALK surgery, indications, techniques, and the…Read article
Perform Microscopy Analysis for Pathology Ergonomically and Efficiently
The main performance features of a microscope which are critical for rapid, ergonomic, and precise microscopic analysis of pathology specimens are described in this article. Microscopic analysis of…Read article
How to improve your Biomarker Discovery Workflow with Laser Microdissection
Biomarkers can be used as indicators of certain diseases, such as cancer. The tumor microenvironment moved into the spotlight in this concern. It is in close interaction with the tumor itself.…Read article
Studying Human Brain Development and Disease
Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…Read article
How to Conduct Standard-Compliant Analysis of Non-Metallic Inclusions in Steel
This webinar will provide an overview of the significance of non-metallic inclusions in steel and outline the important global standards for rating the quality of steel and difficulties that arise in…Read article
Plastic & Reconstructive Surgery: Why Use a Microscope
Plastic and Reconstructive Surgery procedures can be delicate. Visualization solutions play an essential role, allowing to perform the surgery in the best conditions. And more and more plastic…Read article
Minimally Invasive Spine Surgery: Improving Precision and Accuracy with microscopes
Spine surgery is extremely delicate and requires extensive training and experience. Innovative visualization technologies can also help achieve better outcomes allowing to see more and have a clearer…Read article
Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry
Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these…Read article
Neurosurgery with Heads-up Display
In the following video interviews Prof. Dr. Raphael Guzman, Vice Chairman of the Department of Neurosurgery at the University Hospital in Basel, Switzerland, talks about his experience in heads-up…Read article
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…Read article
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…Read article
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…Read article
Key Factors to Consider When Selecting a Stereo Microscope
Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting, observing, documenting, or dissecting samples.…Read article
Challenges Faced When Manually Rating Non-Metallic Inclusions (NMIs) to Determine Steel Quality
Rapid, accurate, and reliable rating of non-metallic inclusions (NMIs) is instrumental for the determination of steel quality. This article describes the challenges that arise from manual NMI rating,…Read article
OCT-Analysis of a Clear Corneal Incision
The aim of this prospective study was to analyse the effect of lateral stromal hydration on the morphology of clear corneal incision architecture using the microscope integrated anterior segment OCT.Read article
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.Read article
How to Quantify Label-free Blood Flow
The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging.Read article
How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…Read article
Studying Natural Killer (NK) Cells Derived from Induced Pluripotent Stem Cells (iPSC)
The study of natural killer (NK) cells holds tremendous promise for developing novel immunotherapies. NK cells derived from induced pluripotent stem cells (iPSCs) can be used to create an easily…Read article
Time-resolved STED microscopy
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…Read article
Criteria to Consider When Choosing a Digital Solution for Steel Quality Rating
For the efficient and fast rating of non-metallic inclusions to determine steel quality, deciding which digital rating solution to use can be a challenge. This article discusses the important criteria…Read article
Upright Fluorescence Microscopy for Virus Replication Studies
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 and spread over the world quickly. Due to its dramatic impact, researchers are interested in the nature of the…Read article
Augmented Reality (AR) Fluorescence Image Gallery
Building on a decade of leadership in fluorescence imaging technology, GLOW800 AR fluorescence is the first of many modalities based on the proprietary GLOW AR platform. The sophisticated imaging…Read article
Obtain maximum information from your specimen with LIGHTNING
LIGHTNING is an adaptive process for extraction of information that reveals fine structures and details, otherwise simply not visible, fully automatically. Unlike traditional technologies, that use a…Read article
Studying Adipose Tissue Development and Expansion
This article discusses sharp, high-contrast imaging of whole mount adipose (fat) tissues specimens, which are thick and round (unsectioned), with a THUNDER Imager using Computational Clearing. Adipose…Read article
Study synaptic dynamics with millisecond precision in neurobiology
What if you could dissect the synaptic dynamics with millisecond precision? To resolve controversial questions in neurobiology employing new techniques for visualizing cellular activity is essential.…Read article
Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody
Vaccines and targeted therapeutics for treatment of the respiratory disease COVID-19, caused by the coronavirus SARS-CoV-2, are currently lacking. Based on the results of this study, it was reported…Read article
Advanced Techniques in Cataract and Refractive Surgery
In this webinar Dr. Thompson and Dr. Moshirfar will explain how Leica microscopes aid in procedures such as Centration of Multifocal IOLs and corneal inlays such as Kamra and Lenticular Grafts used in…Read article
Augmented Reality and Fluorescence: Clinical Uses in Cerebrovascular and Skull Base Neurosurgery
In this webinar Dr. Bendok and Dr. Morcos explain how Augmented Reality and Fluorescence can enhance visualization and support surgical decision making. They present first-hand experience of the GLOW…Read article
Expanding the frontiers of confocal live cell imaging
To address the specific challenges of live cell confocal imaging and help researchers get closer to the truth, Leica Microsystems has re-imagined confocal microscopy, designing its entirely new…Read article
Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…Read article
What is intraoperative OCT telling us?
In the following videos are excerpts taken from a webinar delivered by Dr. Barbara Parolini. During the webinar Dr. Parolini discusses and illustrates how intraoperative OCT provides real-time,…Read article
From dentistry to micro-dentistry: See more – optimize your dental workflow
Endodontics expert Fabio Gorni shares his vast experience in micro-dentistry and provides answers to questions of the webinar participants at the end of the recording. This webinar allows you to…Read article
Industrial Microscopy: Digital imaging and the Leica DVM6
This webinar will discuss digital microscopy and Leica’s digital DVM6 microscope. We will navigate the difference between optical and digital magnification, explain the differences in optics, and…Read article
Reasons Why There is Growing Need for Fast and Reliable Steel Quality Rating Solutions
Steel quality is critical for the manufacturing of high-quality components and products. Fast, reliable, and accurate detection and classification of inclusions has become essential for both component…Read article
The Benefits of Fluorescein and ICG use in Vascular Neurosurgery
In this webinar Professor Marco Cenzato, head of neurosurgery in the Niguarda Hospital in Milan, Italy talks about the use of Fluorescein Sodium and Indocyanine Green (ICG) in Vascular Neurosurgery.Read article
Inverted Microscopes for Grain Size Analysis: Three Factors to Consider
Microscopic steel grain size analysis is useful in determining the quality of steel alloys for a given purpose such as building bridges vs railroad rails. This webinar will describe the preparation of…Read article
Optimized Vision in Endodontic Surgeries and Procedures
Microscopes are playing an increasingly important role in endodontic surgery as they enable minimally invasive procedures to be carried out. Endodontists can work with high precision from the first…Read article
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read article
Depth Profiling and Layer Analysis for Inspection of Materials with a 2-In-1 Solution Combining Optical Microscopy and Laser Spectroscopy
In addition to simultaneous visual and chemical inspection, a 2-methods-in-1 materials analysis solution, which combines optical microscopy and laser induced breakdown spectroscopy (LIBS), can also be…Read article
Microscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read article
IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is known that IL-18 is produced in immune and…Read article
Surgical Microscopes: Key Factors for OR Nurses
Operating room (OR) nurses are vital to the surgery process. An OR Nurse Manager explains the key surgical microscope features to facilitate their work.Read article
Observing the effect on transcription factor signaling and chromatin landscapes in cells infected with the human coronavirus 229E
Viral infection triggers an inflammatory cellular response which is characterized by altered gene expression. In this study, the influence of coronaviruses on cellular NF-κB (transcription factor)…Read article
Top Issues Related to Standards for Rating Non-Metallic Inclusions in Steel
Supplying components and products made of steel to users worldwide can require that a single batch be compliant with multiple steel quality standards. This user demand creates significant challenges…Read article
Covid-19: Mitigating Risks in Dentistry
The coronavirus pandemic poses significant challenges for dental professions. Learn how microscopes can help increase physical distance and minimize risks.Read article
Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer
Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent relapses and drug resistance that leads to…Read article
Analyzing non-metallic inclusions in steel
Oftentimes we find ourselves caught up in tedious analyses by reticle and comparison chart, time-consuming double-evaluation according to several standards or subjective inspection results with a bias…Read article
Performing Elemental Analysis down to the Micro Scale
If you work in electronic component analysis, you will be familiar with the many challenges posed. Whether you are identifying metallic particles or checking product authenticity, it’s important to…Read article
Introduction to Ion Beam Etching with the EM TIC 3X
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the…Read article
How we took the Ophthalmic Microscope to the Next Level
To support surgical decisions and ultimately deliver the best outcomes, ophthalmic surgeons need to have as much visual information as possible. With ever evolving technology, and often restricted…Read article
Computational Clearing - Enhance 3D Specimen Imaging
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…Read article
Visual and Chemical Analysis of Steel Microstructure: Faster Rating of Steel Quality
Simultaneous visual and chemical analysis of steel non-metallic inclusions with a 2-methods-in-1 solution, using optical microscopy and laser induced breakdown spectroscopy (LIBS), is described in…Read article
Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra
In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample.Read article
Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms
Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…Read article
STELLARIS White light lasers
When it comes to choosing fluorescent probes for your multi-color experiments, you shouldn’t have to compromise. Now you can advance beyond conventional excitation sources that limit your fluorophore…Read article
TauSense Technology Imaging Tools
Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments.…Read article
Workflow Solutions for Industrial Research
This brochure presents and explains appropriate workflow solutions for the most frequently required sample preparation methods for material science samples.Read article
Rate the Quality of Your Steel: Free Webinar and Report
This webinar and report describe optimal microscopy solutions for rating steel quality in terms of non-metallic inclusions and reviews the various international and regional standards concerning…Read article
The Power HyD detector family
In STELLARIS, we have pushed the limits of our detector technology, so you can push the boundaries of your research. Our newly designed Power HyD family consists of three different type of detectors…Read article
Simplify and speed up element analysis at the micron range
Learn how to get spatially resolved information about the chemical composition of elements in seconds - quickly and easily.Read article
How to Uncover Hidden Dimensions in Research with Lifetime Imaging
Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based…Read article
How to Drape an Overhead Surgical Microscope
The tutorial features the Leica ARveo digital Augmented Reality microscope for complex neurosurgery. The procedure also applies to the Leica M530 OHX, OH6, OH5 and OH4.Read article
How to Drape a Surgical Microscope
Before performing surgical procedures, it is important to drape the surgical microscope to ensure sterile working conditions. At Leica, we are committed to helping you with your surgical practice. In…Read article
How to Sanitize a Microscope
Due to the current coronavirus pandemic, there are a lot of questions regarding decontamination methods of microscopes for safe usage. This informative article summarizes general decontamination…Read article
Overcoming Complexities in Microdentistry
Dr. Salam Abu Arqub, from the Smile Engineer Dental Center in Amman, Jordan, has been using Leica dental microscopes for three years for all procedures performed at the clinic. He shared his…Read article
Minimally Invasive Dentistry: Visualization & Posture
Microscopes do not only provide better visualization during dental surgery. They also help ensure correct posture to avoid back pain and neck injuries. Dr. Iyad Ghoneim, from the Safad Dental Center…Read article
THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…Read article
Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…Read article
Evaluating Axon Regeneration After Brain or Spine Trauma of Mice
Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…Read article
Ophthalmic Surgical Panel Discussion - Summary
Prof. Kei Shinoda invited four surgeons, all experts in optical performance and microscope systems, to talk about the Proveo 8 ophthalmic microscope from Leica Microsystems. The group discussed topics…Read article
Interview with Magali Mondin on THUNDER Imagers
Magali Mondin, an engineer at the BIC (Bordeaux Imaging Center) in France, describes her experience using a THUNDER Imager in this interview. She had the opportunity to test extensively the THUNDER…Read article
Unruptured Intracranial Aneurysms
Managing unruptured intracranial aneurysms is complex and usually involves one or a combination of three options – conservative, endovascular, or surgical treatment. During surgical treatment the…Read article
Multiphoton Microscopy Publication List
Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development…Read article
How to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…Read article
Key Factors for Efficient Cleanliness Analysis
In the automobile industry, even the smallest particles on single components can cause malfunctioning. Fuel, lubricant and urea filter systems are particularly vulnerable. Component cleanliness has…Read article
GLOW800 Augmented Reality Fluorescence in Aneurysm Treatment
This case study from Prof. Dr. Feres Chaddad talks about the treatment of unruptured MCA (middle cerebral artery) and PCOM (posterior communicating artery) aneurysms with microsurgical clipping. It…Read article
Polarizing Microscope Image Gallery
Polarized light microscopy (also known as polarizing microscopy) is an important method used in different fields, including research and quality assurance. It goes beyond just producing images at high…Read article
Metallography – an Introduction
This article gives an overview of metallography and metallic alloy characterization. Different microscopy techniques are used to study the alloy microstructure, i.e., microscale structure of grains,…Read article
Principles of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking…Read article
Step by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…Read article
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure is described in terms of smooth peripheral…Read article
Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease
Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of…Read article
Metallographic Solutions from Leica Microsystems
Watch this webinar to learn more about obtaining reliable and accurate metallographic results with Leica microscopes in terms of efficient analysis of non-metallic inclusions in steel as well as of…Read article
Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM)
This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019. Dr. Okabe…Read article
Medicinal Use of Qianhu Plant Roots
This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn (Qianhu in Chinese). The qualitative and quantitative…Read article
Brief Introduction to Surface Metrology
This report briefly discusses several important metrology techniques and standard definitions commonly used to assess the topography of surfaces, also known as surface texture or surface finish. With…Read article
Drosophila Testis Niche Stem Cells – Three Color Computational Clearing
Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…Read article
Chat with Dr. Thomas Mathivet about In Vivo Cardiovascular Imaging of the Brain
Dr. Thomas Mathivet, working at the Cardiovascular Institute in Paris, France, was interviewed by Dr. Luis Alvarez, Product Application Manager, during Leica Microsystems Meets Science 2019 in…Read article
Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy
This work reports new interesting findings concerning porous media infiltration which have direct implications for the fields of energy (oil recovery) and environment (CO2 sequestration and aquifer…Read article
Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells
Intracellular dynamic events, such as protein trafficking, recycling, and degradation as well as signal transduction, to name a few, can be studied using live-cell imaging techniques. However,…Read article
SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM
SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel-by-pixel…Read article
3-Dimensional Imaging of Macroscopic Defects in Aluminum Alloys
The investigation of macroscale defects in aluminum (Al) alloys with a rapid 3-dimensional (3D) imaging approach is described in this report. Aluminum (Al) alloys play an important role in the…Read article
Studying the Microstructure of Natural Polymers in Fine Detail
The potential of cryogenic broad ion beam milling used in combination with scanning electron microscopy (cryo-BIB-SEM) for imaging and analyzing the microstructure of cryogenically stabilized soft…Read article
Introduction to Ultramicrotomy
When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types: scanning electron microscopy (SEM) which images the…Read article
Improve Cryo Electron Tomography Workflow
Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…Read article
Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging
Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…Read article
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an…Read article
How to improve clinical practice with dental microscope solutions from Leica Microsystems
Prof. Sleiman talks about the most important features for good imaging during his dental procedures using a Leica dental microscope. A large depth of field and a powerful illumination are very…Read article
Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…Read article
Cleanliness Analysis with a 2-methods-in-1 solution
This report explains how a 2-in-1 materials analysis solution, combining optical microscopy and chemical analysis by laser induced breakdown spectroscopy (LIBS), leads to an overall more efficient and…Read article
How to Adapt Grain Size Analysis of Metallic Alloys to Your Needs
Metallic alloys, such as steel and aluminum, have an important role in a variety of industries, including automotive and transportation. In this report, the importance of grain size analysis for alloy…Read article
How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development
In her latest studies, Dr. Mukta Deobagkar-Lele investigated the role of ZIP7, a zinc transporter protein, in modulating B cell activity and its effects on human immunity. During an interview with Dr.…Read article
Improved tree-ring visualization using autofluorescence
Autofluorescence can be a very practical tool for the visualization of growth rings in wood, especially when these structures are hard to distinguish, like in tropical trees. Tree rings are largely…Read article
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read article
Digital Classroom Options
As teachers, you know your big challenge is to catch and keep the students’ attention and the best chance for this is by making the environment interactive. In the case of the Microscopy Classroom, we…Read article
Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…Read article
Single-residue bioorthogonal labeling of G protein-coupled receptors (GPCRs) in live cells for quantitative analysis
To perform quantitative studies of membrane bound G protein-coupled receptors (GPCRs) with high-performance microscopy, the proteins must first be labeled with bright and photostable dyes. The…Read article
Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow
Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…Read article
Visualize developmental patterns in Drosophila
The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within biology is the development of multicellular life.…Read article
Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation
Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…Read article
How to create EDOF (Extended Depth of Focus) images
Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…Read article
Automated topographic analysis with the Leica Map software
Watch this video to learn how you can save time when doing topographic analysis using the Leica Map software. The software allows you to go quickly from automated analysis of multiple sets of acquired…Read article
Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)
The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy) provides a 2D graphical view of lifetime…Read article
Surgeons describe how Proveo 8 helps to streamline their workflow
Experienced ophthalmic surgeons explain how they perform cataract surgery with high efficiency using the Proveo 8 ophthalmic microscope from Leica Microsystems. The sophisticated illumination system…Read article
Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed
Leica Microsystems‘ Workshop on Coherent Raman Scattering (CRS) Microscopy attracted 30 enthusiastic participants from all over the world to beautiful Konstanz, Germany! The group enjoyed a number of…Read article
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…Read article
Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue…Read article
How to make a fast Z-stack
Save time for your 2D and 3D analysis. Watch this video to learn about the new user interface, LAS X.next, for the DVM6 digital microscope. The video demonstrates how to make a fast Z-Stack with a few…Read article
How can PAULA help streamline the workflow for my wound healing assay?
The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell…Read article
Use of EnFocus Intraoperative OCT to overcome difficulties in visualization during Synechiolysis
The aim of this study was to describe intraoperative optical coherence tomography (OCT) as an adjunctive device for peripheral synechiolysis in a post-penetrating keratoplasty patient.Read article
How to improve your RNA Analysis Workflow with Laser Microdissection
Brain research: Single neuron excision for RNA analysis - Improve your workflow with laser microdissection - Parkinson’s disease is a common progressive neurodegenerative disorder. It is connected…Read article
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…Read article
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…Read article
Improve 3D Cell Biology Workflow with Digital Light Sheet Microscopy
Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach…Read article
Improve Workflow in Kidney Research with 3D STED Deep Nanoscopy
Visualize the ultrastructure of the glomerular filtration barrier. The combination of optical clearing and nanoscopy allows to study the glomerular filtration barrier at the nanoscale. The Leica TCS…Read article
Cryo-SEM analysis of UV light stimulated sun screen lotion
The EM ICE is the only available high-pressure freezer combining light stimulation with fast cryo fixation, a module mostly used for optogenetic approaches in neurobiology studies. However, this…Read article
What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…Read article
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…Read article
How to Develop an Airway Epithelial Cell Model
The present study describes the optimal growth conditions for the proliferation and differentiation of bovine bronchial epithelial cells (BBECs). The cells are grown at an air-liquid interface (ALI)…Read article
Real time images of 3D specimens with sharp contrast free of haze
THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…Read article
Integrated Cryo-tomography Workflow
Analyzing purified protein structures in a cellular context is key to understanding how proteins and protein complexes function. Thermo Fisher Scientific and Leica Microsystems developed the…Read article
Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue
Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…Read article
How to improve your Alzheimer Protein Analysis with Laser Microdissection
Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination.…Read article
What is a Tandem Scanner?
A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the…Read article
Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity
An international consortium of researchers has found a new mutation in a gene harboring a reticulum-to-cytoplasm zinc transporter. The gene is called ZIP7 and it is responsible for the early onset of…Read article
Confluency Check with PAULA Cell Imager
Many cell-based experiments require cells in a certain state. This can include their morphology, fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells…Read article
Extending Nanoscopy Possibilities with STED and exchangeable fluorophores
When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…Read article
Using Mounting Frames for DLS Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general…Read article
How to improve your DNA Mutation Analysis Workflow with Laser Microdissection
DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. To find and understand the underlying mutation for a…Read article
Using a Rotation Device for DLS Sample Mounting
The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due to its unique optical architecture samples can be…Read article
A Molecular Link between Cell Migration and Vascular Disease
Blood vessels transport vital nutrients and oxygen to all the cells in the body. Guided by a complex signaling network, endothelial cells sprout, proliferate, and migrate to form those vessels. One of…Read article
Quick 3D imaging of alloy defects
Aluminum (Al) alloys play an important role in the production of aircraft and vehicles, as well as products in other industries. Defects present in the Al alloy used for the production of aircraft,…Read article
Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data
The Cell Atlas, a part of the Human Protein Atlas, was created by the group of Prof. Emma Lundberg at the SciLifeLab, KTH Royal Institute of Technology, in Stockholm, Sweden. Currently, she is a…Read article
Array Tomography for SEM 3D Reconstruction
Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute…Read article
Expanding the Limits of Electron Microscopy Sample Preparation
Capturing the intricate changes in fine structure or in cell dynamics with conventional cryo solutions can be challenging sometimes. Leica Microsystems has developed a new cryo platform, the Leica EM…Read article
See More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…Read article
You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research.
Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University, Faculty of Agriculture, in 2004 and received his PhD…Read article
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read article
SP8 DIVE Multiphoton Microscope Image Gallery
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…Read article
MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)
Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating…Read article
Live Cell Isolation by Laser Microdissection
Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections,…Read article
High Resolution Array Tomography with Automated Serial Sectioning
The optimization of high resolution, 3-dimensional (3D), sub-cellular structure analysis with array tomography using an automated serial sectioning solution, achieving a high section density on the…Read article
Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10–100 Mammalian Cells
Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of…Read article
Macroscale to Nanoscale Pore Analysis of Shale and Carbonate Rocks
Physical porosity in rocks, like shale and carbonate, has a large effect on the their storage capacity. The pore geometries also affect their permeability. Imaging the visible pore space provides…Read article
Observing Malaria Infection at the Right Spot in the Human Host
Malaria is a life-threatening disease transmitted through the bites of mosquitoes infected with protozoan parasites. The most common and dangerous type of malaria is caused by the parasite Plasmodium…Read article
Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope
Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by…Read article
Super-resolved STED spectroscopy
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.Read article
Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector
Bloodsucking insects play a major role in the transmission of pathogens that cause major tropical diseases. Their capacity to transmit these diseases is directly associated with the availability and…Read article
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.Read article
STED Nanoscopy at the forefront of cancer research
Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope…Read article
What Does 30,000:1 Magnification Really Mean?
One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful guidelines to determine the useful range of…Read article
See the Structure with Microscopy - Know the Composition with Laser Spectroscopy
The advantages of a 2-in-1 materials analysis solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) for simultaneous visual and chemical inspection are described in…Read article
FLIM FRET and Biosensors: Versatile Tools for Biomedical Research
Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of…Read article
Researchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning
Neurons react to learning and memory by activating synaptic connections. The mechanisms behind this fundamental process are complex and poorly understood. Researchers at Thomas Jefferson University…Read article
mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve…Read article
High-Resolution 3D Imaging of Whole Organ after Clearing
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have…Read article
Visualization of DNA Molecules
Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low…Read article
Leica is MAD about LIBS
When analyzing material for cleanliness testing, users normally just want a simple way to know whether the particle under scrutiny is normal debris or something more risky. Users can now “LIBS” it to…Read article
Lifetime – a Proper Alternative
„Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in…Read article
FCS - Fluorescence Correlation Spectroscopy
FCS is a fluorescence-based measurement method. Fluorescent molecules passing through a strongly focused, fixed laser beam are excited for fluorescence emission. After passing a confocal pinhole, the…Read article
FLIM FRET - Förster Resonance Energy Transfer
A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and estimates intermolecular distances on an Angström…Read article
What is FLIM - Fluorescence Lifetime Imaging?
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.Read article
SP FLIM - Spectral Fluorescence Lifetime Imaging
The SP8 FALCON is the ideal tool for spectral FLIM detection. No emission filters in front of the FLIM detectors are necessary. This grants a much higher flexibility to the experimental design.Read article
FCCS - Fluorescence Cross-Correlation Spectroscopy
FCCS (Fluorescence Cross-Correlation Spectroscopy) can be measured using the Leica TCS SP8 FCS system. Similar to FCS , it analyzes fluorescence intensity fluctuations derived from a small…Read article
Solvent immersion imprint lithography
We expand upon our recent, fundamental report on solvent immersion imprint lithography (SIIL) and describe a semi-automated and high-performance procedure for prototyping polymer microfluidics and…Read article
Role of circadian gene Clock during differentiation of mouse pluripotent stem cells
Biological rhythms controlled by the circadian clock are absent in embryonic stem cells (ESCs). However, they start to develop during the differentiation of pluripotent ESCs to downstream cells.…Read article
KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity
Pathologic electrical remodeling and attenuated cardiac contractility are featured characteristics of heart failure. Coinciding with these remodeling events is a loss of the K+ channel interacting…Read article
Effect of curcumin analogs onα-synuclein aggregation and cytotoxicity
Alpha-synuclein (α-Syn) aggregation into oligomers and fibrils is associated with dopaminergic neuron loss occurring in Parkinson’s disease (PD) pathogenesis. Compounds that modulate α-Syn aggregation…Read article
How to Conduct Localized Proteomics of Microscopic Regions
In this webinar, you will learn: - How to perform isolated proteomics of microscopic regions of interest - How to use this technique with FFPE tissue - How proteomics can help you understand…Read article
Coupling of vinculin to F-actin requires Syndecan-4 proteoglycan
Heparan Sulfate Proteoglycans act as a cell surface co-receptors for many proteins in which a signal is sent downstream via its cytosolic domain. The role of syndecan-4 proteoglycans in FA assembly…Read article
The Future of Fluorescence in Vascular Neurosurgery
In vascular neurosurgery, surgical microscopes are used to provide a magnified and illuminated view of the surgical field. Although surgeons benefit greatly from the superb image quality and optical…Read article
California NanoSystems Institute at UCLA Publications
A list of the published scientific articles which include work done in the ALMS/MSI Facilities.Read article
Abstracts of the 7th European Super-Resolution User-Club Meeting
The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of…Read article
Cleanliness Workflow from Leica and Pall
Obtaining cleanliness results rapidly, accurately, and reliably is a significant advantage for manufacturers and component suppliers. For this reason, Pall Corporation and Leica Microsystems have…Read article
DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting
The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical…Read article
The Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…Read article
Multiphoton Microscopy – a Satisfied Wish List
The colorful picture shows colon tumor cells, fluorescently labelled and lineage traced from a multicolor tracer. The gray color codes for the second harmonic generation (SHG) signal from Collagen 1.…Read article
Practical Applications of Broad Ion Beam Milling
Mechanical polishing can be time consuming and frustrating. It can also introduce unwanted artifacts when preparing cross-sectioned samples for electron backscatter diffraction (EBSD) in the scanning…Read article
Revealing Cellular Dynamics with Millisecond Precision
What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy?Read article
Digital Microscopy in Earth Science
Classical polarized light (compound) microscopes can only be used for prepared samples, because the working distance they offer is insufficient for whole samples. This means that thicker and bigger…Read article
Eyepieces, Objectives and Optical Aberrations
For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the eyepieces. Of course, this is assuming that the microscope…Read article
Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection
Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…Read article
Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…Read article
Introduction to Mammalian Cell Culture
Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…Read article
Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the…Read article
Graphene-Based Microbots for Toxic Heavy Metal Removal and Recovery from Water
Heavy metal contamination in water is a serious risk to the public health and other life forms on earth. Current research in nanotechnology is developing new nanosystems and nanomaterials for the fast…Read article
Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo
The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent…Read article
Basics in Component Cleanliness Analysis
For automotive manufacturers and auto parts suppliers, obtaining component cleanliness results rapidly, accurately, and reproducibly over the entire workflow is a significant advantage. Pall and Leica…Read article
Collecting Light: The Importance of Numerical Aperture in Microscopy
Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the…Read article
Interview with Dr. Shigeki Watanabe on Research in Synaptic Membrane Dynamics
Dr. Shigeki Watanabe, principle investigator of the department of Cell Biology at the Johns Hopkins University School of Medicine in Baltimore, held a workshop in Zürich, Switzerland on methods to…Read article
Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…Read article
Introduction to Widefield Microscopy
One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of interest is exposed to the light source with the…Read article
Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device
What do you associate with the word sensor? Perhaps technologies delivering automation in factories and other production sites? That may be what comes to mind, but advances in organic electronics are…Read article
Microscope-Integrated OCT Feasibility and Utility With the EnFocus System in the DISCOVER Study
This article published in the journal OSLI Retina discusses the findings of the latest phase of the DISCOVER study relating specifically to the EnFocus instrasurgical OCT system from Leica. One of the…Read article
Video: Genome Engineering with CRISPR-Cas9
Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and…Read article
Video: Genome Editing with CRISPR-Cas9
This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease.Read article
Optimization of the Interplay of Optical Components for Aberration free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…Read article
Five Questions Asked: Prof. Dr. Jacco van Rheenen speaks about the most important considerations when imaging deep into mouse tissue
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…Read article
Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…Read article
What is Photomanipulation?
The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their…Read article
Quality with Confidence
From routine quality assurance to advanced 3D analysis of complex surface structures, there is a digital microscope solution to meet almost any requestRead article
Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…Read article
How Intrasurgical OCT can Impact the Surgical Approach to Membrane Peeling
When treating patients with vitreomacular traction (VMT), peeling of the inner limiting membrane (ILM) or an epiretinal membrane (ERM) is often indicated. Successful outcomes depend on precise…Read article
Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…Read article
Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds
Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience,…Read article
Work Efficiently in Developmental Biology and Medical Research with Stereo Microscopy: Rodent and Small Animal Surgery
This report provides information which can help improve the routine work of scientists and technicians performing studies involving surgery on small animals and rodents, i.e. mice, rats, hamsters…Read article
Full-HD Image Injection
The human skull is an especially delicate area for surgery. To differentiate healthy tissue from tumor cells, or treat an aneurysm for example, the surgeon needs a complete picture of the surgical…Read article
Navigating Through the Brain
One of the challenges of neurosurgery is orientation at the surgical site. When resecting tumors, removing arteriovenous malformations, or clipping aneurysms, surgeons often have to work near healthy…Read article
Sylvian dissection with ICG, viewed with FL800 fluorescence
In vascular and neoplastic surgery sylvian dissection is an essential skill. The neurosurgeon needs to recognize the superficial sylvian vein (SSV) in order to safely and accurately achieve a wide…Read article
Micro-CT of Insect Larva Protocol
Species: red blood worm (midge larva) Critical point drying of midge larvae with subsequent X-ray micro-computed tomography (micro-CT) to reconstruct the inner anatomy.Read article
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…Read article
Five Big Mysteries about CRISPR’s Origins
Francisco Mojica was not the first to see CRISPR, but he was probably the first to be smitten by it. He remembers the day in 1992 when he got his first glimpse of the microbial immune system that…Read article
Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are poorly understood. Here we define intercellular…Read article
Testing the Münch Hypothesis of Long Distance Phloem Transport in Plants
Long distance transport in plants occurs in sieve tubes of the phloem. The pressure flow hypothesis introduced by Ernst Münch in 1930 describes a mechanism of osmotically generated pressure…Read article
Video Talk by Kurt Thorn: The Abbe Diffraction Experiment
This lecture describes the famous experiments of Ernst Abbe which showed how diffraction of light by a specimen (and interference with the illuminating light) gives rise to an image and how collection…Read article
How to Turn Microscope Workplaces Ergonomic
Microscopes are tools that affect those who work with them every day. They can be highly demanding for the human body, requiring concentration and a lot of steady activity from many of our muscles. In…Read articleContrast Enhancement of Polycrystalline Metals - Sample Preparation for SEM
Application Note - Ion milling is a perfect alternative for chemical etching, especially for polycrystalline metals, such as copper. Ion milling can be used to increase the contrast of the grain…Read article
Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions
Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being…Read article
Drosophila larvae - Sample Preparation for Cryo-SEM
Application Note for Leica EM ACE900 - Drosophila larvae were sandwiched between two 3 mm aluminum slit carriers with the 100 μm cavities facing each other and high-pressure frozen with a Leica EM…Read article
Giardia lamblia - Sample Preparation for Cryo-SEM
Application Note for Leica EM ACE900 - A 100 mesh copper grid (12 um thickness) was dipped into a concentrated Giardia suspension and sandwiched between two flat 3 mm aluminum specimen carriers with…Read article
Cross Sectioning of Cadmiumsulphide (CdS) for Cathodoluminescence
Cathodoluminescence can be used to achieve spectra and high resolution images of impurity and structural defects in semicondoctors, minerals and insulating materials. This application note explains…Read article
Picea abies (L.) KARST - Sample Preparation for TEM
Application Note for Leica EM AMW - Plants (5-years old) were grown in pots filled with soil and kept in greenhouse conditions. Five weeks before harvesting the plants were transferred into growth…Read article
Cross Sectioning of Copper for Electron Backscattered Diffraction (EBSD)
Application Note for Leica EM TIC 3X - Electron Backscattered Diffraction (EBSD) is a surface technique creating diffraction patterns (Kikuchi-bands). It can be used for crystal orientation mapping,…Read article
Porous Ceramics - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ceramic membrane filters with pore sizes down to a few nanometres must be investigated in cross-section with regard to the structure of the pores. The smallest…Read article
Maple (Acer saccharum) Leaves - High Pressure Freezing and Freeze Substitution for TEM
Application Note for Leica EM HPM100 - Leaves were immersed in hexadecene and placed under a gentle (0.3 bar) vacuum for 10 minutes to evacuate the internal air spaces. The leaves were then trimmed to…Read article
Digital Microscopy with Versatile Illumination and Various Contrast Methods for More Efficient Inspection and Quality Control
State-of-the-art digital microscopes utilizing a versatile illumination system capable of achieving multiple contrast methods, such as the Leica DVM6, are very useful for inspection, quality control,…Read article
Removal of Surface Layers - Sample Preparation for SEM and TEM
Application Note for Leica EM RES102 - Sometimes it is necessary to remove surface layers to gain access to the real surface structure. That can be a native oxide, or layers coming from the…Read article
Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…Read article
Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence…Read article
Cross Section of Solar Cells
Application Note for Leica EM TIC020, Leica EM TIC 3X - Cross section of a complete solar cell.Read article
Cross Sectioning of a Multilayer System - Preparation of a Perfect Sample Surface for EBSD
Application Note for Leica EM TIC 3X - Electron Backscattered Diffraction (EBSD) is a surface technique creating a diffraction pattern (Kikuchi-bands). It can be used for crystal orientation mapping,…Read article
High-Pressure Freezing and Freeze Substitution of Hep-2 Cells Infected with Chlamydia pneumoniae
Application Note for Leica EM HPM100 - Hep-2 cells infected with Chlamydia pneumoniae were cultured on carbon-coated 6 mm Sapphire discs. Cells were high-pressure frozen in an EM HPM100 using the 6 mm…Read article
Multilayer Systems with Widely Different Sputter Rates - Sample Preparation for TEM
Application Note for Leica EM RES102 - The multi-layer system to be prepared in cross-section consists of a Si substrate, a TiN layer with a thickness of a few nm and a 500 nm W layer. All these…Read article
Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy as a Straightforward Tool to Study Host-Pathogen Interactions
Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution.…Read article![High resolution XTEM lattice [110] image of conventional prepared InP](https://webcdn.leica-microsystems.com/fileadmin/_processed_/b/b/csm_Compound_3_525d192774.jpg "In-Containing Compound Semiconductors - Sample Preparation for TEM")
In-Containing Compound Semiconductors - Sample Preparation for TEM
Application Note for Leica EM RES102 - Previous studies showed that surface accumulation of In occurs when InP was milled in a conventional way with Ar ions. The consequence is In islands on the…Read article
MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer
Application example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the post-transcriptional level. Deregulation of miRNAs has been…Read article
Good Reasons why to use a Dental Microscope
With its high magnification and coaxial illumination, a dental microscope provides enhanced visualization that supports diagnosis and treatment. Its documentation capabilities also play a major role…Read article
Carbon Coating for Polymeric Materials
Application Note fo Leica EM ACE600 - A solid understanding of polymer property-structure relationships is critical to improve and shorten development routes to new products. A direct way to determine…Read article
Microscope Resolution: Concepts, Factors and Calculation
In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can…Read article
What is OCT and how can it help ophthalmologists acquire high resolution information on ocular tissue?
Optical Coherence Tomography (OCT) is a non-invasive, non-contact imaging modality used to visualize and monitor changes to the morphology of biological tissue. OCT employs low-coherence…Read article
How Intrasurgical OCT can Provide Additional Information During Ophthalmic DMEK Procedures
Replacing diseased Descemet’s membrane with a donor graft is an especially delicate procedure. Accurate positioning and full adherence to the stroma are crucial for optimal patient outcomes. These…Read article
Multispectral Phloem-Mobile Probes: Properties and Applications
Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had…Read article
High-Resolution Carbon Coating: How much Carbon is too much?
Application Note for Leica EM ACE600 - Carbon support films are routinely used for high resolution TEM. Thickness is one of the main criteria to assess its usefulness for a particular experiment.…Read article
Each Atom Counts: Protect Your Samples Prior to FIB Processing
Application Note for Leica EM ACE600 - Focused ion beam (FIB) technology has become an indispensable tool for site-specific TEM sample preparation. It allows to extract electron transparent specimens…Read article
Factors to Consider when Selecting Student Microscopes for Schools and Universities
Selecting educational microscopes is not an easy task for teachers. The microscopes must stand up to daily use by not always careful hands, be constantly up and running, and fit the budget…Read article
Commercially Available Hand Creams - Sample Preparation for Cryo-SEM
Application Note for Leica EM HPM100 - Hand creams having different water contents were applied into the 100 μm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards.…Read article
"Shallow Trench Isolation" Structures - Sample Preparation for TEM
Application Note for Leica EM RES102 - The cross-sectional preparation of structured semiconductor materials requires a very thorough mechanical pre-preparation. In doing this, it must be ensured that…Read article
Tobacco Leaf - Critical Point Drying Protocol for SEM
Application Note for Leica EM CPD300 - Critical point drying of tobacco leafs with subsequent platinum coating and SEM analysis.