Download Application Note
How to gain deep insights into the development of cancer using spectrally tuneable 2-photon microscopy
Understand the complexity of cancer spread by using the right labelling method and imaging techniques.
About the Application Note
Lineage tracing in a confetti animal model combined with multiphoton imaging using the STELLARIS 8 DIVE allows researchers to follow the progression of cancer cells whereas multiphoton imaging is an adequate technique for intravital imaging and deep penetration into tumor tissue.
4Tune, the spectral non-descanned detector in the STELLARIS 8 DIVE, is flexible and enables an optimal setup of detection ranges for each fluoropore, simplifying complex experiments significantly.
Related stories
Show:
Cross Section Ion Beam Milling of Battery Components
Sample Preparation of Lithium battery systems requires high quality surface preparation to evaluate their internal structure and morphology. Due to the brittle materials involved, preparing pristine…Read article
How to Successfully Perform Live-cell CLEM
The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…Read article
How to Successfully Implement the Leica Nano Workflow
The live-cell CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…Read article
Laser Microdissection Publication List
This list presents current publications in the major application fields for laser microdissection and is updated monthly.Read article
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…Read article
Be Confident in your Results with Cell DIVE Validated Antibodies
The Cell DIVE System includes a carefully curated list of hundreds of commercially available antibodies validated to offer optimal specificity and sensitivity in multiplexed imaging. That validation…Read article
Overcoming Multiplexed Imaging Roadblocks
Whether a single slide or a batch of slides processed on multiple Cell DIVE imagers, every step carried out by the Cell DIVE Acquisition Software eliminates distortions and artifacts and seamlessly…Read article
Intraoperative OCT in Retinal Procedures
The surgical team at Retina Clinic in São Paulo, Brazil, has been using microscope-integrated intraoperative OCT for the past year. In their white paper “Intraoperative Optical Coherence Tomography:…Read article
Optimal Visualization in Brain Surgery
This case study “Treatment of the Galassi type III arachnoid cyst with the M530 OHX surgical microscope from Leica Microsystems” documents the procedure step by step and shows the visualization…Read article
Benefits of Combining STED and Lifetime
In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…Read article
Role of Mucins and Glycosylation in Dry Eye Disease
This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…Read article
Skeletal Muscle Adaptation and Fibrotic Diseases
The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…Read article
Improved Visualization of the Anterior Chamber Angle with Proveo 8
In this interview, Michael G. Richie, MD offers his expert opinion on the Proveo 8 from Leica Microsystems as a means for outstanding visualization of the anterior chamber during MIGS. He describes…Read article
Intraoperative OCT as a Tool to Treat Diabetic Patients
In this interview, Asheesh Tewari, MD, details his experience with Proveo 8 with EnFocus Intraoperative OCT from Leica Microsystems. He finds it a powerful tool in treating his diabetic patients.…Read article
Spectroscopic Evaluation of Red Blood Cells
Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…Read article
How to use a Surgical Microscope as an Operating Room Nurse
Surgical microscopes play an essential role in the modern microsurgery procedures. It provides the surgeon, assistant and operating room staff with a magnified and illuminated high-quality image of…Read article
How to Improve Live Cell Imaging with Leica Nano Workflow
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…Read article