Dissecting Proteomic Heterogeneity of the Tumor Microenvironment
Thomas P. Conrads, Ph.D. - Director of Women’s Health Research, Inova Health System
This lecture will highlight cutting edge applications in applying laser microdissection and microscaled quantitative proteomics and phosphoproteomics to uncover exquisite intra- and inter-tumor heterogeneity. The paradigm-shifting results offer unprecedented opportunities to speed progress in identifying novel molecular sub-types of cancer, therapeutic targets, prognostic signatures, and companion diagnostics.
- Understand how laser microdissection is used to enrich discrete cellular populations from the tumor microenvironment
- Understand the fundamental workflow for conducting quantitative proteomics and phosphoproteomics
- Understand the benefit of focused expression-based analyses of microcompartment enriched specimens to illuminate stromal- and tumor-specific biology.
THUNDER Imagers for Widefield Microscopy
The New THUNDER Imagers from Leica Microsystems utilize Computational Clearing to eliminate the haze of widefield images by algorithmically removing the out-of-focus data without compromising the raw images. This results in spinning disk-like images with the speed and ease of a widefield system, whether you are creating single images or capturing large image stack for 3D Analysis.
STELLARIS: From Power HyD enhanced photon detection to TauSense and τ-STED
Julia Roberti, Ph.D. and Volker Schweikhard, Ph.D.
Confocal imaging is the current standard for fluorescence imaging in the life sciences. With STELLARIS, we introduce the new Power HyD family of detectors(1) and TauSense technology(2). The new generation of Power HyD detectors is designed for high performance in terms of spectral coverage, sensitivity and dynamic range in confocal laser scanning microscopy. TauSense, allows users to gain access to functional imaging thanks to the revolutionary set of imaging modes, including TauContrast, TauGating, and TauSeparation, based on fluorescence lifetime information.
To address the need to extract molecular information in the cellular context beyond the diffraction limit, we developed -STED. -STED exploits the changes in the spatial distribution of lifetimes due to the stimulated emission depletion (STED) process to perform STED in gentle conditions while keeping the desired resolution. -STED eliminates uncorrelated background contributions and facilitates STED co-localization studies through lifetime-based species separation.
In this talk, we will show how the new Power HyD family contributes to the sensitivity of STELLARIS and how TauSense works and can be applied to study micro-environmental changes (i.e., pH, ion concentration). We will also explain how -STED works and how FALCON (Fast Lifetime CONtrast) fast acquisition is essential in its implementation(3). We will show how the -STED approach delivers cutting-edge resolution and image quality at low light dose, key to studying nanoscale dynamics of cellular processes.
Traditional Confocal. Mitotic COS7 Cells – Cyan: H2B/Yellow: Mitotic Spindle/Red: Golgi/Green: Mitochondria/Magenta: Actin.
STELLARIS. Mitotic COS7 Cells – Cyan: H2B/Yellow: Mitotic Spindle/Red: Golgi/Green: Mitochondria/Magenta: Actin.
Confocal Reimagined: New STELLARIS Confocal Platform
In microscopy, our mission is to empower you to drive progress in science. To get you closer to the truth, we have re-imagined the confocal microscope.
- POWER to see more
- POTENTIAL to discover more
- PRODUCTIVITY to do more