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DMI6000 加焦平面自动跟踪系统, 用于活细胞研究的全自动显微镜 Leica DMI6000 B with Adaptive Focus Control

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Adding PBS with and without AFC

The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added.

AFC switched on

AFC switched on

COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. Notice that over the complete acquisition time no focus drift could be measured.
Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.

AFC switched off

AFC switched off

COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. At the time point of adding the ice cold PBS focus drift occurs.
Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.

TIRF imaging with simultaneous AFC

TIRF imaging with simultaneous AFC

MDCK cells expressing GFP-p75
Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.

Time series with DIC contrast method

Time series with DIC contrast method

MDCK cells (Marwin Darbin Chanin Kidney) were imaged over a time period of 10 hours. AFC was switched on.
Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.