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为室温理想切片设计的超薄切片机和为冷冻无缺陷切片的冷冻超薄切片机 Leica EM UC7

  • Sample Preparation for Materials Science

    Sample preparation is key for observing the finest textures and details on the sample surface in high resolution. The right workflow and tools for the pre-preparation will lead to an excellently…
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  • 细胞培养

    在实验室环境下培养细胞是科学家在细胞生物学、癌症研究、发育生物学等任何类型生命科学及药物研究领域开展工作的基础。了解徕卡如何帮助您在实验室中培养动物细胞。
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  • 地质、环境、古生物和地球科学显微镜

    利用徕卡显微系统提供的多功能、可靠的显微镜系统,研究和发现常规至具有挑战性的环境保护和地球科学问题,提出解决方案。您的工作对于推进我们对周围世界的理解举足轻重,因此必须使用功能强大而且准确的研究工具,良好地记录并再现结果。徕卡的生产线符合人体工学,高品质的显微镜解决方案所提供的模块化系统,有助于精确测量、细致分析各种材料。
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  • 生命科学

    作为生物学教师,您使用组织切片和血液涂片作为显微镜样本,向您的学生展示生物体的结构。 若要使显微镜的观测到达一定效果,显微镜必须是灵活的:例如应能够做到放大倍率和光学部件的变更,或者应能够使用诸如相衬和暗场等各种不同的相衬观察方法。通过软件保存、注释和共享图像能够进一步提升学生的学习体验。
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  • Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation

    Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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  • Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue

    Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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  • Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions

    Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being…
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  • Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast

    Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…
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  • Immuno - Electron Microscopy of High Pressure Frozen and Freeze Substituted Mouse Heart

    Application Note for Leica EM AFS2 - Mouse heart tissue from wild-type (WT) and αT -catenin KO (KO) mice was excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer Leica…
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  • Cell Envelope Structure of a Gram-negative Thermotolerant y-Proteobacterium Acidithiobacillus sp., Strain HV2/2 and its Interaction with Pyrite

    Application Note for Leica EM AFS2 - Cells of the the moderately thermophilic Acidithiobacillus sp.strain HV2/2, were centrifuged at 20,0009xg and then cryo-immobilized by high-pressure freezing on…
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  • Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite

    Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…
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  • Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells

    Application Note for Leica EM AFS2 - Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer. Freeze…
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  • Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”

    In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…
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  • Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa

    Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions.…
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  • Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

    We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…
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  • Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells

    The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale…
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  • Brief Introduction to Ultramicrotomy

    The usual thicknesses for transmission electron microscopic examinations range between 20 nm and 150 nm. Ultramicrotomy is a fast and clean method of producing ultra-thin sections of biological…
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  • Protein Trafficking in Cereal Seeds

    Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to…
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  • Perusing Alternatives for Automated Staining of TEM Thin Sections

    Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…
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  • Atomic Force Microscopy Study of a Stretched Impact Copolymer

    Atomic force microscopy (AFM) is a powerful tool in the suite of nanoscale characterization techniques that provides a variety of information including topography, mechanical properties, and…
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  • Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

    This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with…
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  • Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections

    Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic…
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