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徕卡显微系统荧光显微镜——守住光明!

荧光是生物和分析显微镜中最常用的物理现象之一,主要是因为它具有灵敏度高、特异性强的特点。荧光是冷发光的一种形式。

荧光显微镜甚至允许用户决定单个分子种类的分布、数量及其在细胞内的位置。可以实现共区域化和相互作用的研究,离子浓度以及观察细胞内间的胞吞和胞吐过程。

借助超分辨率荧光显微镜,甚至可以成像亚分辨率结构。

请前往Science Lab阅读更多关于荧光显微镜定量荧光技术超高分辨率显微技术的文章。

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Our experts on solutions for fluorescence applications are happy to help you with their advice.

Fluorescence Microscopes & Imaging Systems 24

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THUNDER Imager Model Organism

THUNDER Imager Model Organism 可在发育或分子生物学研究中对整个生物机体进行 3D 探索。

THUNDER Imager 3D Live Cell 和 3D Cell Culture

THUNDER Imager 可为您提供适用于先进 3D 细胞培养试验的解决方案,无论您想要研究的是干细胞、球状细胞团或是类器官。

THUNDER Imager Tissue

THUNDER Imager Tissue 可对通常用于神经系统科学和组织学研究中的 3D 组织切片进行实时荧光成像。

High Speed High Resolution Monochrome Fluorescence sCMOS Camera Leica DFC9000

Leica DFC9000

用于高速活细胞成像、FRAP (漂白后荧光恢复) 和比率测量等高级应用的 4.2 MP 冷却式单色荧光摄像头

Software platform for life science applications

LAS X - 生命科学

面向生命科学应用的软件平台

荧光体视显微镜

Leica M205 FA 和 Leica M205 FCA

徕卡荧光体视显微镜,可让您可以轻松的对GFP 和 mCherry 等转基因表达进行早期检测,从而选出合适的样品。

正置显微镜 Leica DM4 B 和 DM6 B

正置双目生物显微镜

Leica DM4 M及DM6 M 正置式工业研究级显微镜不仅可满足快速概览样品和详细检查样品的需求,独特,可靠的记忆功能还可即时再现成像参数,轻松完成重复性工作。

Leica DM2500 和 DM2500 LED 显微镜是生命科学常规和研究应用中应对艰巨任务的终极工具。

Leica DM2500 & DM2500 LED 荧光显微镜

配备强大的透射光照明、高品质的光学性能以及技术先进的附件,采用面向应用的模块化设计,可根据不同应用进行配置,以完成微分干涉相衬或高性能荧光等具有挑战性的生命科学研究任务。

The Leica DM1000 LED combines the advantages of a fully ergonomic system microscope with innovative LED illumination, and unlimited possibilities in mobile use.

LED生物显微镜

采用寿命长达10万小时的LED照明,享有专利的高度可调的对焦钮,适用于细胞学、血液学和病理学实验室

Leica DFC3000 G

Leica DFC3000 G

清晰的荧光文档用于常规实验

Leica LMT260 XY Scanning Stage

Leica LMT260 XY Scanning Stage

精准与可重复结果的扫描台

为室温和冷冻理想切片而设计 Leica EM UC7

Leica EM UC7

为室温理想切片设计的超薄切片机和为冷冻理想切片的冷冻超薄切片机

非常符合人体工程学系统的显微镜徕卡 DM1000

Leica DM1000

一款符合人体工程学系统的荧光显微镜,不仅拥有高品质的光学性能,还拥有荧光等先进功能,是细胞学、血液学和病理学临床实验室的理想选择。

Leica TL4000 BFDF

透射光底座在明场和暗场应用中能吸收高品质成像的冲击和振动

Leica M165 FC

荧光体视显微镜

Triple beam technology separates beam bath specifically for fluorescence illumination.

Leica MZ10 F

荧光成像的模块化立体显微镜

LAS X Widefield Systems performs workflow-guided advanced image analysis and includes a full range of image enhancement tools.

适用于徕卡荧光显微镜和宽场显微镜的综合应用系统平台

一款适用于所有徕卡宽场科研显微镜与共聚焦显微系统的软件平台

采用独特人体工学设计且具有智能自动系统的显微镜可提高实验室操作流程 Leica DM3000

Leica DM3000 & DM3000 LED 生物显微镜

采用电动物镜转盘、聚光顶镜、自动光线强度调节装置与可选脚踏开关等配置,适用于病理学、细胞学与血液学研究

荧光显微镜

用于研究应用的荧光显微镜都是基于一套光学滤光片而工作:

  • 一个激发滤光片
  • 一个分束片
  • 一个发射滤光片

这些过滤片通常是一起插在滤光块(复合显微镜)或在一个扁平的盛放器(主要是立体显微镜)中。

激发滤光片会选择波长来激发标本内一个特定的染料,发射滤光片作为一种质量控制,只让特定的荧光团发射的波长通过。二向分色镜是用来反射激发带中的光并在发射带中传递光,从而产生经典的荧光入射光。

这个荧光教程解释了光路中光学元件和荧光显微镜的工作模式,例如可用于透射光对比法的倒置显微镜和荧光显微镜。

用于荧光显微镜的摄像头

为了获得荧光图像,必须使用合适的CCD或sCMOS荧光摄像头。徕卡显微系统为所有应用需求提供专用荧光摄像头。每个摄像头都提供了良好的灵敏度与图像质量的结合,以确保优质的结果。

用于荧光显微镜的软件

在荧光显微镜中,直观的设置和实验控制变得越来越重要。专用的Leica Application Suite (LAS) X 软件平台有助于建立更加复杂的实验。LAS X从实验建立起一直引导您到成像数据的分析。从简单的多通道实验到复杂的三维分析,LAS X是您优质的软件平台。

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