Contact & Support

Is confocal microscopy obsolete?

Super-resolution microscopy is at the forefront of biomedical science today, but does it supersede confocal microscopy?

Of course not! In fact, the opposite is true – good confocal imaging is the ideal base from which to blast off on a journey beyond the diffraction limit.

Confocal Microscopy alone provides better lateral resolution than the Abbe limit, if operated in the appropriate regime. And only confocal microscopes that perform at high sensitivity and low noise can benefit from these confocal-inherent resolution improvements. In combination with sensible and professional image reconstitution algorithms, it is possible to achieve resolution of 140 nm resolution and beyond, at high speed and multiple channels simultaneously, all without having to tinker endlessly with the optical setup.

Find out

what HyVolution Super-Resolution can do for your research

Transform your confocal microscope into a super-resolution microscope

Leica’s HyVolution solution transforms a confocal microscope into a super-resolution microscope. Teaming ingenious inventions with smart implementation has resulted in a confocal microscope with continuously tunable filter functions, the Leica TCS SP8. It seamlessly adapts to any dye or dye combination you may want to employ. As a second pillar, the tunable elements provide maximal transmission. This prevents loss of your precious emission, whilst enabling very thin sectioning conditions. After all, optical sectioning is the main reason for buying a confocal microscope.

Don't overload your optical beam path!

High sensitivity and thin optical sections then merge with scientific image reconstitution to result in super-resolved images with high contrast and very low noise. That is exactly what you need.

HyVolution does not overload the optical beam path with additional elements, no fibers are required to transmit the emission and no optics are needed to adapt the diffraction pattern. All channels can operate in parallel in super-resolution mode, at all magnifications and wavelengths independently, including UV- and IR-excitation. And it is available for non-descanning detector signals too.

If you want to image living samples speed is what matters. The Leica TCS SP8 offers truly parallel channel recording, ensuring no decrease in imaging speed, whatever configuration you choose.

Compare the results: 3 channel simultaneous acquisition of COS7 cells with HyVolution super-resolved (right) versus confocal (left). Green: Mitochondria (Oregon Green 488), red: Actin (SiR), white: Tubulin (TMR). Sample courtesy of Dr. Jana Doehner, Center of Microscopy and Image Analysis, University of Zurich, Switzerland.

News

11. October 2017 White Blood Cell Recruitment to Bacterial Infection

Zebrafish In Vivo Study Using Leica Fluorescence Microscopes

Tile scan image of Mouse brain tissue section with multicolor immunofluorescent labeling

23. May 2017 Find the Missing Links in Advanced Live Cell Research - Leica launches DMi8 S live cell imaging solution

Wetzlar, Germany – Leica introduces the DMi8 S, a new complete solution designed to give researchers the ultimate tool for fast, versatile microscopic imaging of living cells allowing scientists to find, observe and interact with living cells like...

11. April 2017 Full Spectral Freedom for Multi-color Deep Tissue In Vivo Imaging

Leica Microsystems launches SP8 DIVE – The World's First Tunable Deep Imaging Solution

01. November 2016 Explore life’s true nature with super-resolution and nanoscopy

With HyVolution 2 and the Leica TCS SP8 STED ONE, Leica Microsystems paves the way to access imaging beyond the diffraction limit

21. May 2015 GSD Around the World – A Travel Diary on Facebook

Tamara Straube and Peter Laskey will take you on a journey around the world: Together with a team of specialists, they visited 10 countries, organized 11 workshops for GSD super-resolution microscopy, imaging and sample preparation, and met more than...

13. March 2015 EMBL Advanced Course – Intensive Super-Resolution Microscopy Course for Localization Microscopy and STED

From 20th to 25th July 2015 the EMBL (European Molecular Biology Laboratory), Heidelberg will be holding a six-day intensive course on the subject of super-resolution microscopy in cooperation with Leica Microsystems.

19. January 2015 New Leica TCS SP8 at Bates College: „Second of its kind in Maine, new microscope is a game-changer“

According to Bates College, the confocal offers new imaging capabilities and supports research in biology, neuroscience, nanotechnology and photophysics.

03. December 2014 Free Infographic: Which Super-Resolution Microscopy Method is Right for You?

This illustrated guide to super-resolution microscopy helps you compare technologies to best fulfill your individual research needs.

The Leica TCS SP8 STED 3X was chosen among The Scientist Top 10 Innovations 2014

01. December 2014 The Scientist Top 10 Innovations Award for Leica TCS SP8 STED 3X

Leica Microsystems’ STED Super-Resolution Microscope Again Awarded for its Innovative Technology

Leica HyD SMD – the universal detector for SMD and imaging.

19. November 2014 Universal Hybrid Detector for Single Molecule Detection and Imaging at SfN and ASCB

Leica HyD SMD - the Optimal Detector for Precise and Reliable SMD data