Piece of cartilage from the hip of a mouse were cryofixed with the Leica EM PACT and freeze-substituted in acetone containing 0,5 % glutaraldehyde and 0.2 % uranyl acetate and low-temperature embedded in Lowicryl HM20. Sections were labelled for -Cop, detected with protein A gold 10 nm.

Courtesy of:
E.G. van Donselaar, B.M. Humbel
Electron Microscopy & Structure Analysis, Department of Molecular Cell biology, Utrecht University, The Netherlands

J.W. Slot
Department of Cell Biology, University Medical Center Utrecht, The Netherlands

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