The sections are of yeast frozen with a Leica EM HPM100 in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer so that a final MES concentration of 50mM and a dextran concentration of 20% was achieved .....
(by Dr. Jonathan O’Driscoll, Dr. Daniel Kofi Clare and Prof. Helen Saibil, prepared at the Department of Cryostallography, Birkbeck, University of London)
This Application Note describes the preparation method
"CEMOVIS of Yeast"
using Leica EM HPM100, Leica EM UC7 , Leica EM FC7
<media 40149>Click here to download the Application Note</media>
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