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CLEM: Combining the Strengths of Light and Electron Microscopy

Dr. Paul Verkade, School of Medical Sciences, University Walk, Bristol, UK

In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in combining these techniques. This combination is called Correlative Light Electron Microscopy (CLEM).

Due to the high resolution made possible by electron microscopy, artefacts induced during preparation of a sample can, however, also be clearly seen. The Leica EM PACT2 with RTS is a high pressure freezer designed for CLEM experiments to allow excellent preservation of ultrastructure, thus avoiding such artefacts ...

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