Correlative Light Electron Microscopy (CLEM) Using High Pressure Freezing

“With the introduction of Green Fluorescent Protein (GFP) technology, cell biology and life sciences in general have entered a whole new exciting era of research. [...] In some instances however, the resolution of the light microscope is the limiting factor in answering our scientific questions. In these cases, the higher resolution of the electron microscope is essential. Combining both light and electron microscopy is my field of interest.

By performing so-called Correlative Light Electron Microscopy (CLEM) experiments one has the advantage of live cell imaging in the confocal microscope and afterwards have high resolution results from the transmission electron microscope of the same cell.

The Leica EM RTS was specifically developed to be used in such experiments in conjunction with Leica EM PACT2. It provides a high time resolution between the light and electron microscope, allowing excellent preservation of the ultrastructure close to the natural state, an essential prerequisite for electron microscopy. It allows us to decide upon the exact moment of interest and study that particular event at high resolution.”

Dr. Paul Verkade, Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany Dr. Verkade works with the Leica EM PACT2 & EM RTS High Pressure Freezer.

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