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It also reviews potential pitfalls and problems encountered when developing the procedures, as well as useful tricks for solving them. The briefing ends with a peak into the future.

The urge to go deeper into the microscopic world has led researchers to combine the versatility of light microscopy with the resolution power of the electron microscope. The correlative light and electron microscopy CLEM allows scientists to spot cellular structures and processes of interest in whole cell images with LM and then zoom in for a closer look with EM. This dual examination provides valuable complementary and often unique information. With the Leica EM PACT2 High Pressure Freezer Leica Microsystems has contributed a tool for the Rapid Transfer System. This currently most popular method, developed by Paul Verkade, allows to take live specimens from light microscopes to freezing in less than 5 seconds.

The Essential Knowledge Briefing can be downloaded as an pdf-file after registration: http://www.essentialknowledgebriefings.com/portfolio/correlative-light-and-electron-microscopy/

An abstract is available on Leica Science Lab.

The webinar by Paul Verkade “Correlative Light Electron Microscopy: 1 + 1 = 3” can be downloaded on Leica Science Lab. In this webinar, Verkade highlights the aspects of the three basic steps - probes, processing, and analysis – in CLEM.

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