(by Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zürich, Switzerland)
This Application Note describes the preparation method
"Hep-2 cells infected with Chlamydia pneumoniae"
using the Leica EM HPM100.
Protocol:
Hep-2 cells infected with Chlamydia pneumonia grown on carbon coated 6 mm Sapphire disc. High-pressure frozen in a Leica EM HPM100 with ethanol as synchronization fluid ...
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