Microinjections have been performed under a Leica S6 E stereo microscope.
Successful microinjection was verified by monitoring lucifer yellow dextran fluorescence in the heart of the zebrafish using a Leica M205 FA stereo microscope.
The article provides a detailed protocol for intravenous microinjection and can be transferred to other types of studies that require delivering soluble substances into the bloodstream of zebrafish larvae.
