FLIM identifies environmental changes, both spatially and temporally resolved.
A GFP fusion protein was expressed in living cells that localizes to different cellular structures. Subcellular localization of this GFP fusion protein gives rise to two protein populations.
Each population exhibits a different average lifetime as revealed by the lifetime changes with localization to different organelles (yellow and red lines).
We thank Dr. Matthias Weiss, Dr. Jedrzej Szymanski and Nina Malchus, DKFZ, Heidelberg, Germany