Picovitro Plates and Leica HCS A
Fredrik Hellborg, Ph.D., Picovitro
Today, the combination of Picovitro plates with the Leica HCS A solution enables researchers to grow and analyze large numbers of single physically isolated cells or clones of cells and overcome the obstacle of identifying and analyzing rare events or cells.
Performing individual experiments in parallel
The Picovitro plate is a small cell culture plate with many wells and a glass bottom. Each well is 0.5 μl in volume, which is small enough for many cell types to survive as single cells but large enough to allow the cells to expand into clones of a few hundred cells if required. In this way it is possible to perform many individual experiments in parallel in a small format, and to find and study rare events of interest. Each Picovitro plate has 672 wells and is the same size as a standard microscope slide. The glass bottom makes the plate excellent for analysis by most types of microscopy and/or other imaging techniques.
Identifiying events of interest
While Picovitro provides the platform for performing the experiments, Leica Microsystems provides the tools to analyze the experiments to find what is interesting. The automated high content screening solution from Leica Microsystems provides fully automated screens of Picovitro plates. The image analysis software identifies wells that contain events of interest such as cell division or chromosome condensation. These cells can be retrieved from the well or analyzed in more detail by, for example, imaging the particular well with higher magnification.
By combining the Picovitro plates with automated high content screening, research and biotechnology labs are able to perform new cellular experiments which have not been possible with commonly available resources until now. This new solution leads to new ways of investigating cell behavior and destinies – and studying cell cultures at a new level of detail.
Design & dimension of Picovitro plates
Picovitro plates are constructed of three layers: a glass bottom, a silicon microgrid that forms the wells, and a semipermeable membrane to facilitate gas exchange and avoid evaporation.
Outer measures [mm] 75 x 25
No of wells: 672
Wellpattern: 14 x 48
Well volume [nl]: 500
Bottom plate [mm]: 0.17
Complete assembly [mm]: 0.87