(by Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zurich, Switzerland)
This Application Note describes the preparation method
using the Leica EM HPM100.
Cerebellar slices were cultured on Millicell filter membranes. Discs of 4 mm were cut a a 4 mm biopsy punch on a teflon surface. The filder discs containing the brain slices were transferred with a tweezers into the 100 micrometer cavity of a 6 mm aluminium specimen carrier ...
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