(by Dr. sc. nat. Andres Kaech, Center for Microscopy and Image Analysis, University of Zurich, Switzerland)
This Application Note describes the preparation method
"Mouse cerebellum"
using the Leica EM HPM100.
Protocol:
Cerebellar slices were cultured on Millicell filter membranes. Discs of 4 mm were cut a a 4 mm biopsy punch on a teflon surface. The filder discs containing the brain slices were transferred with a tweezers into the 100 micrometer cavity of a 6 mm aluminium specimen carrier ...
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