Improved high throughput capability
More and more users need to collect a large number of dissectates from multiple specimens. This capability is especially advantageous for researchers who work with biobanks and in the field of proteomics. Several new features in the software have been added to meet this growing demand.
Collect many specimens at one time
First, now researchers can collect 352 different specimens in one run. The new LMD software can accommodate 384-well plates*, rather than just 96-well plates which was the case before. To save users time when coping with the higher number of dissectates and specimens, several software features enable certain processes to be automated. One example is the “Inspect all collectors” function. With the press of a button, the LMD system will then automatically inspect and document all collector wells which were used.
Import ROIs at higher speed from external images
Second, users can now define ROIs for laser microdissection with an external imaging method and then import them more quickly in XML format into the new LMD software. For example, researchers working in biobanks often image and annotate hundreds or even thousands of specimens with a slide scanner. Now they can import at greater speed the ROIs from the slide-scanned images.
Select ROIs more rapidly
Third, users now can select more easily ROIs in the field of view (FOV) which should be modified. The new software allows the ROIs to be selected by simply drawing a box around them.
In addition, to improve the accuracy of the 3D position of imported ROIs, z-coordinates of the reference points on the LMD slide are now incorporated. To further improve cutting accuracy, an Active Focus Correction has been implemented. It automatically autofocuses before each cut to eliminate potential errors due to focus drift during a high-throughput experiment. Lastly, users can set a focus for each individual contrast method to compensate for possible focus variation.
*Only 352 wells reachable by the travel range of the stage. Due to the small well opening diameter, the collection rate might be diminished.